##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295193_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1501318
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.886170684691717	31.0	30.0	33.0	25.0	34.0
2	29.692560803240887	31.0	30.0	34.0	25.0	34.0
3	30.006276485061793	31.0	30.0	34.0	25.0	34.0
4	32.94180846429604	35.0	33.0	37.0	23.0	37.0
5	33.4987058038337	35.0	35.0	37.0	27.0	37.0
6	33.56146865620741	36.0	35.0	37.0	27.0	37.0
7	33.62071193444693	37.0	35.0	37.0	27.0	37.0
8	33.73442601767247	37.0	35.0	37.0	27.0	37.0
9	34.514039663815396	39.0	35.0	39.0	23.0	39.0
10	34.66690401367332	39.0	35.0	39.0	25.0	39.0
11	35.012812075789405	39.0	35.0	39.0	27.0	39.0
12	35.05414442509848	39.0	35.0	39.0	27.0	39.0
13	34.873098171073686	39.0	35.0	39.0	26.0	39.0
14	35.496109418524256	39.0	35.0	41.0	24.0	41.0
15	35.81623013911776	39.0	36.0	41.0	25.0	41.0
16	35.795835392635006	39.0	36.0	41.0	25.0	41.0
17	35.54741966725238	39.0	35.0	41.0	25.0	41.0
18	35.489714370972706	39.0	35.0	41.0	24.0	41.0
19	35.909207776100736	39.0	35.0	41.0	26.0	41.0
20	35.96309709202181	40.0	35.0	41.0	26.0	41.0
21	36.031754764813314	40.0	35.0	41.0	26.0	41.0
22	35.95439673673399	40.0	35.0	41.0	26.0	41.0
23	35.71141423735678	39.0	34.0	41.0	25.0	41.0
24	36.03078761461596	39.0	35.0	41.0	26.0	41.0
25	34.94061950899143	38.0	33.0	41.0	23.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.10130584182803304
1101	2	0.04922830355804564
1101	3	0.06481659892808267
1101	4	-0.06627918582637449
1101	5	-0.17726857236007731
1101	6	-0.35184908401803483
1101	7	-0.18502966105259588
1101	8	-0.14823743594011773
1101	9	0.13749981591467275
1101	10	0.12150494222787955
1101	11	0.04914045480054341
1101	12	0.009378504788308817
1101	13	0.09611971203019465
1101	14	0.4118921520415242
1101	15	0.09216283267384284
1101	16	0.09618806402419011
1101	17	0.191858295179145
1101	18	0.4144962714300462
1101	19	0.24381748609751241
1101	20	-0.02308354873361651
1101	21	0.006671299058055524
1101	22	-0.12264910897506809
1101	23	-0.0956122646308799
1101	24	-0.13914541780221157
1101	25	0.05302701305546975
1102	1	0.03266444734246221
1102	2	0.2700171243294811
1102	3	0.15027650628894662
1102	4	0.22384091217650592
1102	5	-0.13939976441849922
1102	6	-0.2607272596442698
1102	7	-0.14689081285598604
1102	8	-0.012252373563008234
1102	9	0.20106826901358232
1102	10	0.5239242199013603
1102	11	0.28761042659372293
1102	12	0.22524410256949778
1102	13	0.12342612197387837
1102	14	0.46005567615613785
1102	15	0.1892194781236256
1102	16	0.21626281952237036
1102	17	0.15134332858125532
1102	18	0.372215682927596
1102	19	0.24721379400876486
1102	20	0.3268119728096295
1102	21	0.2682052018287422
1102	22	0.10032058587102455
1102	23	0.3349466226752398
1102	24	0.10044649291705099
1102	25	0.05812307528567828
1103	1	-0.36179635725244097
1103	2	-0.2852993837383728
1103	3	-0.49947664279857307
1103	4	-0.5554868884513979
1103	5	-0.7178503751137484
1103	6	-0.9900590585615987
1103	7	-0.6268439111826183
1103	8	-0.3494185598554793
1103	9	-0.01332957121476852
1103	10	-0.09751895397555899
1103	11	-0.22748068225362772
1103	12	-0.2753469489110856
1103	13	-0.30091789780200884
1103	14	-0.22760090902654895
1103	15	-0.22155695491545657
1103	16	-0.4405198438946769
1103	17	-0.19315867577861923
1103	18	0.21862678356976062
1103	19	-0.27756934224946406
1103	20	-0.298918410365566
1103	21	-0.27769968060416517
1103	22	-0.33769167323203675
1103	23	-2.5804622632622554
1103	24	-1.353688433705642
1103	25	-0.6857673646304576
1104	1	-0.022280148758994756
1104	2	-0.009419986753446352
1104	3	-0.1347404970840067
1104	4	-0.36792653767792416
1104	5	-0.11284425206151383
1104	6	-0.21601587344835593
1104	7	-0.27424337348009686
1104	8	-0.06761035918827929
1104	9	-2.494003247720549
1104	10	-0.5751296967651314
1104	11	-0.2255023029622123
1104	12	-0.11131249090677642
1104	13	0.05016313762723712
1104	14	0.46036060723824335
1104	15	0.3216633482811986
1104	16	0.00842023464930719
1104	17	0.3646788310834239
1104	18	0.40138511303219815
1104	19	0.05195221511720405
1104	20	-0.03984010567685914
1104	21	0.08957154949591484
1104	22	0.07653492192864064
1104	23	0.09785439003223217
1104	24	0.022993998061032528
1104	25	0.00813984649519739
1105	1	-0.3466788598718118
1105	2	-0.23067682224558084
1105	3	-0.32474053970593886
1105	4	-0.15279767027048763
1105	5	-0.1647652224776337
1105	6	-0.23195540997748765
1105	7	-0.2392390473541326
1105	8	-0.22458160217164647
1105	9	-2.3133363423710662
1105	10	-0.7601696122032209
1105	11	-0.31897432805800463
1105	12	-0.2658958402233367
1105	13	-0.13268174838040636
1105	14	-0.17505352875128466
1105	15	-0.06202673061199704
1105	16	-0.2793406648439216
1105	17	-1.4709656764414802
1105	18	-0.22695165136655504
1105	19	-0.33353544370276467
1105	20	-0.24139815035316303
1105	21	-0.27075318699399986
1105	22	-0.23706658859258312
1105	23	-0.05957823535083406
1105	24	-0.1445805157292952
1105	25	-0.7780653525279106
1106	1	-0.28618119197627934
1106	2	-0.4828756853541698
1106	3	-0.3333224252767373
1106	4	-0.1986593459442716
1106	5	-0.24852264402131397
1106	6	-0.406076740320124
1106	7	-0.31992811075113536
1106	8	-0.3634229048609896
1106	9	-3.0361634803619104
1106	10	-0.671058172176302
1106	11	-0.60170107058849
1106	12	-0.1647416655050833
1106	13	-0.2117117665164585
1106	14	-0.3811870472520411
1106	15	-0.5092551580746374
1106	16	-0.5349135798450675
1106	17	-0.17619429577167978
1106	18	0.030515955755689106
1106	19	-0.1007525113878529
1106	20	-0.21314679471751674
1106	21	-0.32968812409646375
1106	22	-0.13764188671060396
1106	23	-0.09733084193249653
1106	24	-0.3738119947997376
1106	25	-0.5439172673204382
1107	1	-0.36569371866614375
1107	2	-0.13262448661902937
1107	3	-0.2517659535846164
1107	4	-1.5359032525278948
1107	5	-0.41416050410362004
1107	6	-0.37386789073644877
1107	7	-0.12352579381221318
1107	8	-0.20991757427606217
1107	9	0.11915823872401887
1107	10	0.3537813192201398
1107	11	0.0950614210074292
1107	12	-0.5820488889463604
1107	13	-0.11444281394130229
1107	14	0.16522856844344602
1107	15	-0.4363458334298258
1107	16	-0.8283055754712549
1107	17	-0.10016493507245627
1107	18	-0.1389172216866683
1107	19	-0.0446025752957695
1107	20	-0.4117810863001381
1107	21	-0.05680539696478348
1107	22	-0.13776083669989703
1107	23	0.03954845707526289
1107	24	-0.10359160485064223
1107	25	-0.3117693703710316
1108	1	0.19060637783916334
1108	2	0.2106098912717549
1108	3	0.2783232013189014
1108	4	0.7083636821535109
1108	5	0.4293833908105995
1108	6	0.24606620563885429
1108	7	0.1536794524763323
1108	8	0.16826372569543224
1108	9	0.4675058978452711
1108	10	0.5398055505528632
1108	11	0.2067775653953703
1108	12	0.21313884637908842
1108	13	0.20480074445440977
1108	14	0.12886106823093968
1108	15	0.26556827184596443
1108	16	0.21496480439963506
1108	17	0.4447170334358077
1108	18	0.4944466133242571
1108	19	0.24338426398038848
1108	20	0.18095351410288174
1108	21	0.2147005358615246
1108	22	0.22946136052095056
1108	23	0.33159701974621214
1108	24	0.17972947780889825
1108	25	0.3657963957461092
1109	1	0.2498487886107803
1109	2	0.17974126666813106
1109	3	0.17934415381275315
1109	4	0.44042415889436626
1109	5	0.3010708519136429
1109	6	0.27951462457019005
1109	7	0.06953854423857564
1109	8	0.08455698158060443
1109	9	0.4604560405909268
1109	10	0.31697513634350116
1109	11	0.121398869567102
1109	12	0.27186559464292515
1109	13	0.00673052742173752
1109	14	0.4303344602431096
1109	15	0.11750842733500377
1109	16	0.20569522749973856
1109	17	0.4345485046545292
1109	18	0.2463783720100139
1109	19	-0.0743275577307756
1109	20	-0.03355665590768808
1109	21	0.10906331727791496
1109	22	0.08171120987242375
1109	23	0.3143351679053339
1109	24	0.20532639173672607
1109	25	0.1359823108494851
1110	1	0.15482558128869428
1110	2	0.160569913425622
1110	3	0.3042361370112552
1110	4	0.4582506904272563
1110	5	0.41393111676783434
1110	6	0.35627271705863706
1110	7	0.37195050177368927
1110	8	0.3537654264394092
1110	9	0.7871234089908796
1110	10	0.7660072456534905
1110	11	0.49114815029049197
1110	12	0.43215701444984234
1110	13	0.2894653825138178
1110	14	0.5487123536397931
1110	15	0.302597096347462
1110	16	0.2954363881665856
1110	17	0.5910144756027762
1110	18	0.7501015683048635
1110	19	0.4368555645813359
1110	20	0.5184873928928511
1110	21	0.5478405971592224
1110	22	0.49955528473734034
1110	23	0.6727985725147221
1110	24	0.5483581802543682
1110	25	0.4067455961642139
1111	1	0.09016605022710422
1111	2	0.10402180831460939
1111	3	0.1863489457149754
1111	4	0.6067745061726768
1111	5	0.4037952367733908
1111	6	0.30313964476079036
1111	7	0.20512851354886408
1111	8	0.13208430619160083
1111	9	0.739435607704479
1111	10	0.4871643895605331
1111	11	0.22150924484304113
1111	12	0.1376142620633729
1111	13	0.11183740299380673
1111	14	0.2427361993267212
1111	15	0.20909096650476755
1111	16	0.01925254188082448
1111	17	0.26007884439501794
1111	18	0.4478987241340633
1111	19	0.18933407474647623
1111	20	-0.23391201045460974
1111	21	-0.2014593500348525
1111	22	0.00638075706458352
1111	23	0.29275410937952273
1111	24	0.3939644685148451
1111	25	0.3114291961130391
1112	1	0.139723908973707
1112	2	0.1612614628224769
1112	3	0.26951112324801585
1112	4	0.705036466189604
1112	5	0.5836914381927656
1112	6	0.44619270710192893
1112	7	0.3617995804020495
1112	8	0.35335742233226597
1112	9	-0.9396804534480054
1112	10	0.2583442460052936
1112	11	0.12034066850890213
1112	12	0.19331401974988438
1112	13	0.08392013997720227
1112	14	0.4986206907244579
1112	15	0.18377355335622525
1112	16	0.21792062631050158
1112	17	0.29696797317156154
1112	18	0.34372194984139526
1112	19	0.2738707209553084
1112	20	0.3892318924906206
1112	21	0.2996998262071031
1112	22	0.2636681869513495
1112	23	0.2305447036270678
1112	24	0.25894174657159397
1112	25	0.28624537208816037
1113	1	0.11287184540193707
1113	2	0.20494949427060405
1113	3	0.18449337115560027
1113	4	0.4030750430531498
1113	5	0.3268547529595125
1113	6	0.40296796865075635
1113	7	0.2687454383883008
1113	8	0.24707352535069305
1113	9	-0.04684958876891443
1113	10	0.3177224669502223
1113	11	0.10638458968134046
1113	12	0.05195386125386392
1113	13	0.20010655114525377
1113	14	-0.1370511762815596
1113	15	0.11809604214628422
1113	16	0.2371572489742988
1113	17	0.4340972204599254
1113	18	0.30085389718141897
1113	19	0.37633319795878606
1113	20	0.3105373425598401
1113	21	0.2674401868483258
1113	22	0.3449526941844496
1113	23	0.017876778596843224
1113	24	0.20051153269462674
1113	25	0.18803821635346196
1114	1	0.16615877857820038
1114	2	0.17479141279485333
1114	3	0.21139657153049285
1114	4	0.5081969259740831
1114	5	0.42595114879750895
1114	6	0.3683215863088094
1114	7	0.16558456660552423
1114	8	0.11721336736856358
1114	9	-0.11307656865179894
1114	10	0.06988810789995625
1114	11	0.1448959118012496
1114	12	0.10195981366187823
1114	13	0.1143423705645219
1114	14	0.6268997273815415
1114	15	0.22964164255902375
1114	16	0.03254882228413436
1114	17	0.35306432022952805
1114	18	0.4018890773440589
1114	19	0.3565924198552963
1114	20	0.36158492427410494
1114	21	0.2724385257307844
1114	22	-0.03721480068359995
1114	23	0.5324797108860508
1114	24	0.2870595452217941
1114	25	0.26739854886659487
1115	1	0.0917935364164677
1115	2	0.17829928956179586
1115	3	0.15017495913573242
1115	4	0.36100418559015424
1115	5	0.42394833441981206
1115	6	0.4343441355518678
1115	7	0.20650614525786892
1115	8	0.21021325306869443
1115	9	0.34056969993647357
1115	10	0.023070674910826483
1115	11	0.03574381152398587
1115	12	0.07534009207446246
1115	13	-0.20575784373063044
1115	14	0.05241915870202973
1115	15	-0.18057837442853497
1115	16	0.030987465496743027
1115	17	0.25852617726707905
1115	18	0.43782560737788856
1115	19	0.15924239737175583
1115	20	0.35353431201691876
1115	21	0.24268787740410858
1115	22	-0.43732167448627024
1115	23	0.22230750136001376
1115	24	0.10197930602706151
1115	25	0.15925820778755906
1116	1	0.5037567975629287
1116	2	0.47813437606352593
1116	3	0.5011200548156758
1116	4	0.6922662615085429
1116	5	0.6459263861066233
1116	6	0.5268028079348923
1116	7	0.4375614547607043
1116	8	0.19232686303509183
1116	9	0.5637703153762672
1116	10	0.33072178515955386
1116	11	0.06152818584169495
1116	12	0.3458723188207742
1116	13	0.1283745620626604
1116	14	0.5571514814054765
1116	15	0.2531661988941494
1116	16	0.35694007280002893
1116	17	0.6535183028956411
1116	18	0.8859996840299829
1116	19	0.4329440500657782
1116	20	0.3636484818473633
1116	21	0.5431772793420109
1116	22	0.5071516871414161
1116	23	0.6063550720086255
1116	24	0.4183018068808906
1116	25	0.42670519352198255
1201	1	0.07998863260556988
1201	2	0.08630305033870656
1201	3	-0.05550388128017403
1201	4	-0.05487836918162259
1201	5	-0.07742730933571806
1201	6	-0.3745273286282469
1201	7	-0.17852458345152655
1201	8	-0.3093866850365856
1201	9	0.4747463488084094
1201	10	0.4825303071081848
1201	11	0.3754894713250465
1201	12	0.27281300472554193
1201	13	0.26619308452195867
1201	14	0.636974576340009
1201	15	0.20161024299819275
1201	16	0.12250536618935826
1201	17	0.43213833957925374
1201	18	0.4165114546795792
1201	19	0.03552831060478212
1201	20	-0.008669207236039256
1201	21	-0.11676082230222562
1201	22	0.10194901062771322
1201	23	-0.07804267473719051
1201	24	-0.13066612133241762
1201	25	-0.028856324352283025
1202	1	-0.18327293292633584
1202	2	-0.4518137169332199
1202	3	-0.3721538482881641
1202	4	-0.29228886308420954
1202	5	-0.564164521877565
1202	6	-0.6391965531938553
1202	7	-0.3725713587881643
1202	8	-0.3993839967988464
1202	9	0.14994857760188296
1202	10	0.3103315730237739
1202	11	0.04789104516604681
1202	12	-0.0760176118584397
1202	13	-0.13622632944221635
1202	14	-0.04864335176185364
1202	15	-0.08784942121647532
1202	16	-0.19452584180692867
1202	17	0.21451327542812493
1202	18	0.24737242391147873
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2211	8	0.08371846064309807
2211	9	-1.6161793303652914
2211	10	0.01935633170356965
2211	11	0.019027879330003827
2211	12	-0.040559142575162355
2211	13	-0.06442129079100312
2211	14	0.3486815704924524
2211	15	-0.08515000719913246
2211	16	-0.05795242362112418
2211	17	0.3651655744235569
2211	18	0.38684168459293033
2211	19	0.24402975764154178
2211	20	0.3041563760343493
2211	21	0.2074696172404913
2211	22	0.09953275628872404
2211	23	-2.0915395812187256
2211	24	-0.6553005429844987
2211	25	-0.27339789801536085
2212	1	-0.18004207333862965
2212	2	-1.7339190788352
2212	3	-0.55986364061377
2212	4	0.031143907253778025
2212	5	0.12117245280604294
2212	6	0.0585869511401782
2212	7	-0.06851706993931828
2212	8	0.019402268846825166
2212	9	0.3922693803559696
2212	10	0.29241103532547896
2212	11	0.0527544525914152
2212	12	-0.13151858938433492
2212	13	-0.1287438613147316
2212	14	0.11454210392646758
2212	15	-0.15445384667587092
2212	16	0.0534149859086952
2212	17	0.4879522852930833
2212	18	0.5549506332579668
2212	19	0.1439480409708196
2212	20	0.35898808968809703
2212	21	0.22868957472714158
2212	22	0.19615340991865082
2212	23	0.4537645547826443
2212	24	0.17280154423855976
2212	25	0.1941445506919095
2213	1	0.1843329151997004
2213	2	0.19750992618404695
2213	3	0.2099610971810435
2213	4	0.5148323294766826
2213	5	0.36713680881658206
2213	6	0.35639070141589
2213	7	0.2964255762775352
2213	8	0.4238613932037367
2213	9	0.5378360563265687
2213	10	0.564339084462361
2213	11	0.09259799846324768
2213	12	0.19700328150366175
2213	13	0.08257150449218642
2213	14	-1.1735076588232474
2213	15	-0.2555761567162236
2213	16	0.15511891793916988
2213	17	0.391212581911887
2213	18	0.23493218690708062
2213	19	0.1430067117736158
2213	20	0.1690710965846165
2213	21	0.3554676900599034
2213	22	0.5322013717768996
2213	23	0.6134225671092608
2213	24	0.36771405950887015
2213	25	0.1967091049283738
2214	1	0.09215226765206452
2214	2	0.22906749937851245
2214	3	0.10478233351922484
2214	4	0.3221057395088778
2214	5	0.25594164169297073
2214	6	0.112156373065595
2214	7	-1.775628250939576E-4
2214	8	-0.13145637498153206
2214	9	-0.11576941078696024
2214	10	0.10596653112757792
2214	11	-0.0707818663636317
2214	12	-0.18037941644355016
2214	13	-0.25809895204188393
2214	14	-0.04884804424427358
2214	15	-0.12954969746702005
2214	16	0.05145399109386517
2214	17	-0.06096807226448675
2214	18	0.18757254369198506
2214	19	-0.09963771505312735
2214	20	0.08343930544803158
2214	21	0.1072267899840682
2214	22	0.26105737184053623
2214	23	0.313454849037214
2214	24	0.32343627981612855
2214	25	0.1427754161182051
2215	1	0.283560861569196
2215	2	0.2082321550322206
2215	3	0.2162174532408052
2215	4	0.5580857724329604
2215	5	0.4400068859103854
2215	6	0.47941908223268115
2215	7	0.3852643230311159
2215	8	0.2634992870743744
2215	9	0.5525848218626734
2215	10	0.4666506190364501
2215	11	0.21265372426108797
2215	12	0.1771870578615733
2215	13	0.018516858824625615
2215	14	0.16120378119023115
2215	15	0.19296188596761965
2215	16	0.23809159264458657
2215	17	0.4399088380891243
2215	18	0.555334853700181
2215	19	0.34094337420714993
2215	20	-0.3919271445944972
2215	21	-0.08290443024206695
2215	22	-0.9237770033062134
2215	23	0.23148019954046362
2215	24	0.15627658886425166
2215	25	0.3823069522799116
2216	1	-0.10926805886684221
2216	2	-0.5031227275869519
2216	3	-0.09537437097501922
2216	4	0.003044378945148196
2216	5	0.004631930111180793
2216	6	0.1730304359112651
2216	7	-0.09009444799040267
2216	8	-0.039213247446603816
2216	9	0.054079569097510216
2216	10	-0.16511432019775185
2216	11	-0.4113824132930475
2216	12	-0.706565966486572
2216	13	-0.5611941218562961
2216	14	-0.504369061082329
2216	15	-0.4626466084424834
2216	16	-0.23040797580359396
2216	17	-0.06628117398926037
2216	18	0.19115216596246398
2216	19	-0.06325513076045297
2216	20	-1.2302116532147593
2216	21	-1.5943983790109755
2216	22	-1.271100336381842
2216	23	-1.6762851034393336
2216	24	-1.3374841286397725
2216	25	-1.4026509634958728
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3056.0
3	340.0
4	328.0
5	281.0
6	333.0
7	425.0
8	144.0
9	258.0
10	609.0
11	1539.0
12	3432.0
13	6389.0
14	10024.0
15	13644.0
16	16355.0
17	17151.0
18	16234.0
19	15237.0
20	13949.0
21	12686.0
22	11972.0
23	11270.0
24	11333.0
25	11401.0
26	11905.0
27	13039.0
28	14477.0
29	17562.0
30	22085.0
31	28318.0
32	37936.0
33	52022.0
34	73052.0
35	106553.0
36	165758.0
37	276494.0
38	503727.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.09593609818733	21.129354478296396	12.84993413043841	20.92477529307786
2	15.730956298334458	24.373827230758234	37.591341564511744	22.303874906395563
3	18.78178085048363	29.797337760596292	29.01327644193495	22.40760494698513
4	12.7610115746637	20.905528735424703	34.814580078314336	31.51887961159726
5	12.18264031755859	41.907825369518896	31.161071927395312	14.748462385527203
6	28.21013558535889	39.59190056761791	17.711977854012655	14.48598599301054
7	23.933903807221444	37.63349804134922	20.22646962303408	18.206128528395258
8	23.243112798953426	37.14332317018143	20.492243271229142	19.121320759636
9	25.341852292004486	20.88189803718166	19.154556404124286	34.62169326668957
10	16.0134922810513	31.934450781255226	28.213576524233286	23.83848041346019
11	29.277508372207677	30.015491840896992	19.936175486015593	20.77082430087974
12	22.774868585062976	31.6103634838576	25.52180000868859	20.092967922390834
13	26.912959435324428	25.975062923989423	25.59761921147739	21.51435842920876
14	23.100208691538135	27.687346262897876	24.97154965662457	24.240895388939414
15	25.88718265483957	30.013831258644537	23.28103660236368	20.817949484152212
16	21.849510385598066	31.83996931892285	24.165156665918346	22.145363629560737
17	19.234630985900427	32.36397108959498	25.193524839364358	23.207873085140236
18	19.4359109727044	29.484661469732252	29.862903845575534	21.21652371198781
19	21.595672724745086	30.999729979735115	26.780797014270973	20.62380028124883
20	23.559720064806	28.652870337893138	27.537939904127207	20.24946969317366
21	24.67760671695561	29.700485377842124	24.63222368820322	20.989684216999052
22	22.923173434150232	29.023295090520758	25.77044024106801	22.283091234261
23	23.68423337761916	28.084761601316682	25.675024864854755	22.5559801562094
24	22.132904071638055	28.714693994011455	25.782096232400036	23.370305701950457
25	23.16360812949025	29.896777711255556	25.51163455781731	21.42797960143688
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23849.0
1	23849.0
2	16294.5
3	8740.0
4	8740.0
5	8740.0
6	9221.5
7	9703.0
8	9703.0
9	9703.0
10	10557.5
11	11412.0
12	11412.0
13	11412.0
14	14244.5
15	17077.0
16	17077.0
17	17077.0
18	23161.0
19	29245.0
20	29245.0
21	29245.0
22	37516.0
23	45787.0
24	45787.0
25	45787.0
26	57770.0
27	69753.0
28	69753.0
29	69753.0
30	81314.0
31	92875.0
32	92875.0
33	92875.0
34	99483.0
35	106091.0
36	106091.0
37	106091.0
38	114311.0
39	122531.0
40	122531.0
41	122531.0
42	165225.5
43	207920.0
44	207920.0
45	207920.0
46	201284.5
47	194649.0
48	194649.0
49	194649.0
50	187379.0
51	180109.0
52	180109.0
53	180109.0
54	159117.0
55	138125.0
56	138125.0
57	138125.0
58	123865.5
59	109606.0
60	109606.0
61	109606.0
62	91416.0
63	73226.0
64	73226.0
65	73226.0
66	53113.0
67	33000.0
68	33000.0
69	33000.0
70	25530.5
71	18061.0
72	18061.0
73	18061.0
74	12338.0
75	6615.0
76	6615.0
77	6615.0
78	4296.0
79	1977.0
80	1977.0
81	1977.0
82	1333.0
83	689.0
84	689.0
85	689.0
86	444.0
87	199.0
88	199.0
89	199.0
90	122.0
91	45.0
92	45.0
93	45.0
94	37.0
95	29.0
96	29.0
97	29.0
98	17.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14274124469299643
2	0.28821342313886866
3	0.3419661923723022
4	0.3697417868832586
5	0.37680224975654725
6	0.33737023069063315
7	0.34203280051261625
8	0.3590844844330115
9	0.3466287621942853
10	0.37620277649372086
11	0.29314242552210795
12	0.33990134002256683
13	0.31112662340689984
14	0.3231160886634278
15	0.3617488100455733
16	0.30839568965402403
17	0.3587514437314413
18	0.3279118747660389
19	0.3419661923723022
20	0.30260078144670216
21	0.34396443658172354
22	0.2983378604666033
23	0.28141939282683615
24	0.31938603280584127
25	0.3158558013691969
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1501318.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.926016333024375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.36376547960212	16.305011364909504
2	7.747198299998227	3.3973039292187956
3	3.3969355121001703	2.2344379056161654
4	2.1122765289303693	1.8525523869276523
5	1.532931617881155	1.6805541845535839
6	1.153369429387586	1.5173278168057929
7	0.9374362599963897	1.4387969923495088
8	0.7640278455205253	1.3401669615814769
9	0.6534124781142436	1.2894059400601354
>10	6.1581001475357535	28.389875272798854
>50	0.7892753135571313	11.89454904961621
>100	0.35058378900323867	13.40151001685485
>500	0.02355580432129167	3.6149251202301835
>1k	0.014684137758727274	5.935216990398898
>5k	0.0012236781465606063	1.8121914158287507
>10k+	0.0012236781465606063	3.896174652249651
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAA	19183	1.277743955644307	No Hit
GTACATAAGCAGTGGTATCAACGCA	17535	1.1679737404067625	No Hit
GCTTATGTACTCTGCGTTGATACCA	10834	0.7216325921623533	No Hit
GTATCAACGCAGAGTACTTTTTTTT	10534	0.7016501500681401	No Hit
GGTATCAACGCAGAGTACTTTTTTT	7327	0.48803784408100087	No Hit
CTTATGTACTCTGCGTTGATACCAC	7223	0.4811105974883403	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6308	0.4201641491009899	No Hit
GTGGTATCAACGCAGAGTACATGGG	6159	0.4102395361941974	No Hit
GAGTACATAAGCAGTGGTATCAACG	4637	0.3088619466362223	No Hit
TATGTACTCTGCGTTGATACCACTG	4160	0.2770898637064233	No Hit
GCGTTGATACCACTGCTTATGTACT	3677	0.24491813193474	No Hit
GTACTGGTTCACTATCGGTCAGTCA	3358	0.22367013517455994	No Hit
CCCATGTACTCTGCGTTGATACCAC	3272	0.21794183510755216	No Hit
CATAAGCAGTGGTATCAACGCAGAG	3117	0.20761757335887535	No Hit
ATACAGGGTGACAGCCCCGTACACA	2761	0.18390507540707565	No Hit
CTGCTTATGTACTCTGCGTTGATAC	2720	0.18117414165419984	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	2680	0.1785098160416381	No Hit
GGATACCACGTGTCCCGCCCTACTC	2519	0.167785905451077	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2491	0.16592087752228374	No Hit
ACTCTGCGTTGATACCACTGCTTAT	2464	0.16412245773380457	No Hit
GTATCAACGCAGAGTACATAAGCAG	2439	0.16245725422595347	No Hit
TATCAACGCAGAGTACATAAGCAGT	2298	0.15306550644167324	No Hit
GGTATCAACGCAGAGTACATGGGGG	2125	0.14154229816734362	No Hit
GGTATCAACGCAGAGTACATAAGCA	2054	0.1368131202050465	No Hit
CATGTACTCTGCGTTGATACCACTG	2000	0.13321628062808813	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	1783	0.11876231417994056	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	1700	0.1132338385338749	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1606	0.10697267334435477	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	1541	0.10264314422394188	No Hit
GTGGTATCAACGCAGAGTACATAAG	1507	0.1003784674532644	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.660814031404406E-5	0.0	0.0	6.660814031404406E-5	0.0
2	6.660814031404406E-5	0.0	0.0	6.660814031404406E-5	0.0
3	6.660814031404406E-5	0.0	0.0	6.660814031404406E-5	0.0
4	6.660814031404406E-5	0.0	0.0	6.660814031404406E-5	0.0
5	4.6625698219830843E-4	0.0	0.0	6.660814031404406E-5	0.0
6	5.994732628263965E-4	0.0	0.0	6.660814031404406E-5	0.0
7	5.994732628263965E-4	0.0	0.0	6.660814031404406E-5	0.0
8	5.994732628263965E-4	0.0	0.0	6.660814031404406E-5	0.0
9	5.994732628263965E-4	0.0	0.0	6.660814031404406E-5	0.0
10	5.994732628263965E-4	0.0	0.0	6.660814031404406E-5	0.0
11	5.994732628263965E-4	0.0	6.660814031404406E-5	6.660814031404406E-5	0.0
12	5.994732628263965E-4	0.0	6.660814031404406E-5	6.660814031404406E-5	0.0
13	5.994732628263965E-4	0.0	6.660814031404406E-5	1.3321628062808812E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTAGG	25	0.006008528	19.012815	1
CGTAGGA	25	0.0060154987	19.009	2
TTAGCGA	25	0.00603645	18.99756	13
CGCTTGG	40	2.7700863E-4	16.617865	19
CCAATTA	80	7.727067E-9	15.435517	14
ATTACCA	75	5.8636942E-8	15.196016	17
GGTAGAC	45	6.736178E-4	14.785271	3
CTAGACC	100	6.530172E-10	14.25818	4
GAAAATC	40	0.00528817	14.246265	17
CGATCAC	75	9.718933E-7	13.927354	19
GACCTAT	55	1.9616549E-4	13.816869	7
TCTTGCA	70	7.2969015E-6	13.56515	18
GTCGTAC	50	0.0014919013	13.30897	1
GCCTCGA	50	0.0015043599	13.2942915	19
GCGCTTG	50	0.0015047388	13.293848	18
CCGGCTG	80	2.0021434E-6	13.0608225	14
ACAGGCC	80	2.0021434E-6	13.0608225	8
GCACATC	75	1.4804484E-5	12.66504	13
ATCGCCG	75	1.4850346E-5	12.661231	19
CAATTAC	100	1.4446778E-7	12.348413	15
>>END_MODULE