FastQCFastQC Report
Wed 25 May 2016
SRR1295193_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295193_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501318
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT305152.0325474016830545No Hit
GGTATCAACGCAGAGTACTTTTTTT220471.4685096695037294No Hit
GTACATAAGCAGTGGTATCAACGCA190771.2706834927710184No Hit
TATCAACGCAGAGTACTTTTTTTTT187311.2476370762223592No Hit
GCTTATGTACTCTGCGTTGATACCA111990.7459445633769793No Hit
ACGCAGAGTACTTTTTTTTTTTTTT93510.622852720076626No Hit
CTTATGTACTCTGCGTTGATACCAC80530.5363953539489967No Hit
GTACTTTTTTTTTTTTTTTTTTTTT73080.48677228941503403No Hit
GTGGTATCAACGCAGAGTACATGGG63270.42142970376695676No Hit
GAGTACATAAGCAGTGGTATCAACG51540.3432983551785831No Hit
GAGTACTTTTTTTTTTTTTTTTTTT46660.3107935827053296No Hit
TATGTACTCTGCGTTGATACCACTG39410.2625026809776476No Hit
GCGTTGATACCACTGCTTATGTACT39090.26037122048759825No Hit
CATAAGCAGTGGTATCAACGCAGAG38300.25510917740278877No Hit
CCCATGTACTCTGCGTTGATACCAC37370.24891462035358264No Hit
GCAGAGTACTTTTTTTTTTTTTTTT36240.2413879004980957No Hit
GTACTGGTTCACTATCGGTCAGTCA36140.24072181909495521No Hit
ATACAGGGTGACAGCCCCGTACACA32140.2140785629693376No Hit
ACGCAGAGTACATAAGCAGTGGTAT31530.2100154664101809No Hit
CTGCTTATGTACTCTGCGTTGATAC29780.19835904185522324No Hit
GTGGTATCAACGCAGAGTACTTTTT29330.1953616755410912No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA28230.1880347801065464No Hit
ATCAACGCAGAGTACTTTTTTTTTT28030.18670261730026552No Hit
ACTCTGCGTTGATACCACTGCTTAT27360.18223987189922455No Hit
GGATACCACGTGTCCCGCCCTACTC26190.17444671948248142No Hit
GTATCAACGCAGAGTACATAAGCAG25740.17144935316834942No Hit
GGTATCAACGCAGAGTACATGGGGG24190.16112509141967257No Hit
TATCAACGCAGAGTACATAAGCAGT24060.16025918559559002No Hit
CATGTACTCTGCGTTGATACCACTG23480.15639591345737544No Hit
GGTATCAACGCAGAGTACATAAGCA21220.1413424737464015No Hit
ACCCTGTATCGCGCGCCTTTCCAGA18840.12548973635165903No Hit
AAGCAGTGGTATCAACGCAGAGTAC18190.12116020723124614No Hit
GTGGTATCAACGCAGAGTACATAAG15880.10577372681870197No Hit
CCCATATTCAGACAGGATACCACGT15820.10537407797681769No Hit
GGTATCAACGCAGAGTACATGGGCA15810.10530746983650366No Hit
ACATAAGCAGTGGTATCAACGCAGA15570.10370887446896661No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCCG250.006031311619.0003685
TGACGCT307.721562E-419.0003664
TCTTACG453.538173E-516.8892142
TCTCCGA350.002169391416.28657211
CCTTTGG601.4623674E-615.8378581
CCTATAC551.1280512E-515.54575352
CTTACGC508.7121894E-515.2002943
CCTATCC704.4347144E-714.9288593
GGTTAAC400.00526899214.2540731
CTCCGAG400.005277150314.2507512
GGTCCGT400.005277150314.2507510
CCTCCGA602.566873E-514.25074911
CTCATAA400.00527831714.2502762
ACTGATC759.666328E-713.9340678
TTACGCT551.9597614E-413.8184484
CTTTTGA551.9619775E-413.81660717
GGTTATA707.240744E-613.5753071
ATCGTCA707.2707044E-613.56988219
ACGGTTC500.001498911413.30078
AACGGTT500.001499287713.3002577
Produced by FastQC (version 0.11.5)