FastQCFastQC Report
Wed 25 May 2016
SRR1295192_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295192_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4221084
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG63130.1495587389400448No Hit
GTATCAACGCAGAGTACTTTTTTTT53010.12558385476337358No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN46560.11030341969029756No Hit
GGTATCAACGCAGAGTACTTTTTTT45020.10665506775036934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT14350.011.9214571
TGGTATC14500.011.535542
GGTATCA32600.011.07834151
TAGGACG803.7578947E-410.6923114
CGCAAGT1409.7203156E-810.17734212
TGTCCGA800.00452088339.49930510
ATCGACC1151.11251844E-49.0861848
CTAATAC4500.08.6591433
AGCGCAA1451.6922168E-58.51661810
GCACCGT1701.615077E-68.3817396
CCAATAC4200.08.3725173
TTAGGAC2658.913048E-118.2487013
GTCTTAT4150.08.2444781
TTATACT6400.08.1677384
TAACACG1752.4266192E-68.1465234
CTGGACT7600.08.1286584
TCCTATA4800.08.1177542
TTAGACT2951.2732926E-118.0544724
CTAGAAC4150.08.0153693
GTATCAA57300.07.94492241
Produced by FastQC (version 0.11.5)