FastQCFastQC Report
Wed 25 May 2016
SRR1295192_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295192_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4221084
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT141020.3340847990705705No Hit
GGTATCAACGCAGAGTACTTTTTTT120710.28596919653814046No Hit
TATCAACGCAGAGTACTTTTTTTTT79560.18848238983161672No Hit
GTGGTATCAACGCAGAGTACATGGG67230.1592718837151784No Hit
ACGCAGAGTACTTTTTTTTTTTTTT51260.12143800028618242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43500.011.9925351
GTGGTAT17200.011.2701461
TCGCGGG600.005883019411.08218715
AGCGTCA3650.010.9324483
TGGTATC16450.010.8003222
TATTCCG1651.0657459E-79.21291355
CAGCGTC4350.09.1732032
ATACCGT1356.857006E-69.1478526
CTAGACT3850.09.1306564
CGAATAC1151.1104491E-49.0877383
GTTCTAG4850.09.0124721
TGAACCG950.00182337999.0007745
GTGACCG950.00182475639.0000289
TAGGACC2553.6379788E-128.9419454
GCAGCGT3650.08.851441
TAGACAG6000.08.7090845
CTATGCG1107.1485084E-48.6363919
TCTGTCG3650.08.5890688
TCTATAC5200.08.5872773
GTATCAA77850.08.5685311
Produced by FastQC (version 0.11.5)