FastQCFastQC Report
Wed 25 May 2016
SRR1295191_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295191_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3713770
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG59270.15959523610778265No Hit
GTATCAACGCAGAGTACTTTTTTTT52000.14001944116086887No Hit
GGTATCAACGCAGAGTACTTTTTTT44330.11936657358964071No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN43530.11721242834101196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28800.012.0794661
GTGGTAT11300.011.8604131
TGGTATC11100.011.6439072
GTTAGAC1053.4540662E-610.861273
CCGACTA803.7779944E-410.686079
GACCGTC750.002652286610.1318427
AGACCGT750.002652286610.1318426
TCGTACA1054.0918607E-59.9560292
CGTACAC800.00450379739.5036113
ACCGCCC1201.704277E-59.49885758
TTAGACA3550.09.1025424
TAAACGT950.00181750319.0039084
CTAGTAC1901.0524673E-89.0034213
CTTAGAC2750.08.9852333
TAGGACC2701.8189894E-128.8001164
CTTACAC3350.08.7943863
GACTGTC3300.08.6350927
CTAATAC4100.08.5764293
AAGACCG1451.6815338E-58.5208245
ACACCGT1451.6935963E-58.5159866
Produced by FastQC (version 0.11.5)