FastQCFastQC Report
Wed 25 May 2016
SRR1295191_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295191_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3713770
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT138790.3737172738214806No Hit
GGTATCAACGCAGAGTACTTTTTTT119840.32269095824458705No Hit
TATCAACGCAGAGTACTTTTTTTTT79970.2153337444160516No Hit
GTGGTATCAACGCAGAGTACATGGG63340.17055445006018144No Hit
ACGCAGAGTACTTTTTTTTTTTTTT51280.138080710437103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT14800.013.48211
AGCGTCA4450.012.8096873
GGTATCA43500.012.3630921
TGGTATC15850.012.1079162
TATGCCG550.003067952612.0918955
TATACCG658.022542E-411.6932615
GCAGCGT4750.011.6020391
TAGACCG600.00587469911.0842365
CAGCGTC5150.011.0685652
TACACCG1201.5237765E-610.2925055
GCGTCAG5900.09.6615444
AGACCGA1054.4945537E-49.0477486
GCTCGTT1054.4945537E-49.04774811
CGTCAGA6100.09.0335235
CGCAAGT1401.0856284E-58.82167212
GTACGGA1000.00290175048.5515031
AAGGTCG1000.00290422778.5506975
CAGGACT5250.08.5052264
TCTATAC4550.08.3521033
TTACACT5350.08.346254
Produced by FastQC (version 0.11.5)