FastQCFastQC Report
Wed 25 May 2016
SRR1295190_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295190_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4598814
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG70830.154017970720277No Hit
GTATCAACGCAGAGTACTTTTTTTT60920.13246893655625122No Hit
GGTATCAACGCAGAGTACTTTTTTT53000.11524710501446676No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN51250.11144177607530985No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA47100.10241771030530915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35700.011.4774431
TACGTCG909.477282E-510.5606165
GTGGTAT15450.010.2759991
ACCGGTC856.606186E-410.0574948
TGGTATC15100.09.8824342
TGGACCG1804.1927706E-88.9765235
GTATCAA65450.08.6135291
GTATTAG5100.08.5747951
ACACCGT1804.0493615E-78.4430526
GTCTTAG3950.08.1831021
GGACCGA1659.775291E-68.0592786
TAAGACT5800.08.0302354
GTATTAA7600.08.0057581
CCTATAC4300.07.9575623
GTGTAAG5900.07.89550971
CTAAGAC4000.07.84151363
TTATACT6250.07.6041414
GTGTTAT5300.07.5337211
CTAATAC5200.07.49422073
CAGTGCG1658.506075E-57.48385959
Produced by FastQC (version 0.11.5)