FastQCFastQC Report
Wed 25 May 2016
SRR1295190_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295190_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4598814
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT167960.36522459921188377No Hit
GGTATCAACGCAGAGTACTTTTTTT146570.3187126072069886No Hit
TATCAACGCAGAGTACTTTTTTTTT95610.20790142849873902No Hit
GTGGTATCAACGCAGAGTACATGGG78230.170109075948712No Hit
ACGCAGAGTACTTTTTTTTTTTTTT61360.13342570497523926No Hit
GGTATCAACGCAGAGTACATGGGGG46210.10048242873053792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT19900.012.6063131
GGTATCA51700.011.7999911
TGGTATC20700.011.5658532
GCGGTAT600.00586719111.0862331
TATACGA850.0074333778.9420575
ACCGACC850.00743735638.9414738
TCTATAC5350.08.7016023
GTATTAG5250.08.6879861
AGCGTCA3400.08.6624293
GGTTAGA3350.08.5096881
TTAGACA5150.08.4859954
TAGACAG5950.08.4630175
GTCTAGG3400.08.3845451
TCGTGTA1701.6132744E-68.38244813
GTATCAA92500.08.3416411
TATACCG1150.00111019988.26168255
ATTAGAC3250.08.1852443
CACACCG1401.0387553E-48.1436595
GACCGTG1659.74803E-68.0609617
CTAGGAC4250.08.0476753
Produced by FastQC (version 0.11.5)