FastQCFastQC Report
Wed 25 May 2016
SRR1295189_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295189_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3366734
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG52000.1544523564974245No Hit
GTATCAACGCAGAGTACTTTTTTTT45330.13464087153900486No Hit
GGTATCAACGCAGAGTACTTTTTTT38630.1147402794518367No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN38400.1140571247980981No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAC751.4814163E-512.6653157
TACGGTC701.08725246E-412.2195415
GTGGTAT12900.011.1999511
TTACGGT600.00585992311.0879374
TAGTACG2100.010.8608432
GGACCGA1053.473955E-610.8559846
AGTACGG2400.010.6913013
AACGCCC803.7846636E-410.683969518
GGTATCA28500.010.672531
GTACGGG1451.4360921E-810.4871144
TGGTATC12700.010.4760092
ATACCGT1151.1126014E-49.0859866
TCTATAC3350.09.0778543
ATAGCGA850.0074459298.9400898
GTCTTAT3100.08.8919821
GTATACG1000.00289179788.55471
GTCCTAA3450.08.5409251
GTATAGA4500.08.4490861
GTCTTGG5300.08.4291591
AGTACTG5000.08.3635975
Produced by FastQC (version 0.11.5)