##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295189_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3366734 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.164504828715305 31.0 30.0 33.0 26.0 34.0 2 30.410182984459123 31.0 30.0 34.0 26.0 34.0 3 30.3358697182492 31.0 30.0 34.0 26.0 34.0 4 33.46489505853447 35.0 35.0 37.0 27.0 37.0 5 33.951394140434026 35.0 35.0 37.0 28.0 37.0 6 34.088648821082984 37.0 35.0 37.0 29.0 37.0 7 34.01261103490801 37.0 35.0 37.0 28.0 37.0 8 34.001858774705696 37.0 35.0 37.0 28.0 37.0 9 35.01461238101971 39.0 35.0 39.0 27.0 39.0 10 35.040808391752954 39.0 35.0 39.0 27.0 39.0 11 35.48618245456873 39.0 35.0 39.0 28.0 39.0 12 35.48236837243453 39.0 35.0 39.0 28.0 39.0 13 35.42675186100239 39.0 35.0 39.0 27.0 39.0 14 36.0969167745358 39.0 36.0 41.0 27.0 41.0 15 36.45095068395662 40.0 36.0 41.0 27.0 41.0 16 36.424279435203374 40.0 36.0 41.0 27.0 41.0 17 36.09162470215942 39.0 36.0 41.0 27.0 41.0 18 36.00161551224421 39.0 36.0 41.0 27.0 41.0 19 36.41603197639017 40.0 36.0 41.0 27.0 41.0 20 36.4352921852454 40.0 36.0 41.0 27.0 41.0 21 36.482026795107664 40.0 36.0 41.0 27.0 41.0 22 36.3863206894278 40.0 36.0 41.0 27.0 41.0 23 36.09076066003433 39.0 36.0 41.0 27.0 41.0 24 36.36697464070521 40.0 36.0 41.0 27.0 41.0 25 35.10093164473344 39.0 34.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.1986760435820507 1101 2 0.012332702896767245 1101 3 -0.2249661548092341 1101 4 -0.24992652277796878 1101 5 -0.25487945438069914 1101 6 -0.35651867758208766 1101 7 -0.2842134221384143 1101 8 -0.2473606085684068 1101 9 -0.21829963503786587 1101 10 -0.08932280236092538 1101 11 -0.023921444630126132 1101 12 -0.1972064107397884 1101 13 -0.06733449876907116 1101 14 0.3657931972248747 1101 15 0.14902840626393044 1101 16 0.07478365358208094 1101 17 0.056877654091628926 1101 18 0.3202478555644106 1101 19 0.033452555022030595 1101 20 0.07131055039349832 1101 21 -0.0774629346278104 1101 22 -0.07207726379045454 1101 23 -0.056873753381289305 1101 24 -0.17872478536731506 1101 25 -0.1862858815428723 1102 1 -0.2019995872096878 1102 2 0.08035421129332221 1102 3 -0.1437199463779244 1102 4 -0.191264422277321 1102 5 -0.34681280513400026 1102 6 -0.43468978735186425 1102 7 -0.3509269418349561 1102 8 -0.3679786352169927 1102 9 -0.3722074318399038 1102 10 0.09434220119214842 1102 11 -0.039123500226473595 1102 12 -0.13026525197643224 1102 13 -0.24791125088669475 1102 14 -0.013783268215277644 1102 15 -0.11028916620313822 1102 16 -0.21638168781837663 1102 17 -0.31785822611568904 1102 18 0.04820340064633655 1102 19 -0.19245779997796575 1102 20 -0.09576916969559335 1102 21 -0.05474524487245702 1102 22 -0.11027397939372463 1102 23 -0.11274272816430653 1102 24 -0.18503576458404325 1102 25 -0.23707371116521614 1103 1 -0.4943959446130677 1103 2 -0.2735798138150223 1103 3 -0.58094508576864 1103 4 -0.49116250626857294 1103 5 -0.49826670440684495 1103 6 -0.6784603824969793 1103 7 -0.6044710522111956 1103 8 -0.4247526573481153 1103 9 -0.14832202665880345 1103 10 -0.00512391524613065 1103 11 -0.15831142619532557 1103 12 -0.38885210485879895 1103 13 -0.2835259124351026 1103 14 -0.439643765072276 1103 15 -0.3340984787114323 1103 16 -0.305410250694095 1103 17 -0.13889619802171893 1103 18 0.08163447727386597 1103 19 -0.27674330945657744 1103 20 -0.2318969597913565 1103 21 -0.34763476285743167 1103 22 -0.3379703281734976 1103 23 -2.6271421554643197 1103 24 -1.2211970517396082 1103 25 -0.6883312485973505 1104 1 -0.3685446404272028 1104 2 -0.060905605193870116 1104 3 -0.3136893332512827 1104 4 -0.6754329152994814 1104 5 -0.44782639715003114 1104 6 -0.45191561800085367 1104 7 -0.41649445008573593 1104 8 -0.4429318089097336 1104 9 -2.641663206072195 1104 10 -0.8331543961874459 1104 11 -0.6207307721813606 1104 12 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8 -0.1418068577105629 1107 9 0.16542652493620125 1107 10 0.41527141597892125 1107 11 0.12344351809393572 1107 12 -0.5260015144778762 1107 13 -0.25252937470872894 1107 14 0.12374960435137439 1107 15 -0.7034447625622775 1107 16 -0.9884093457183383 1107 17 -0.2408837939436097 1107 18 -0.15417748372183837 1107 19 -0.05009521705093789 1107 20 -0.35274228819889686 1107 21 -0.11811593708429058 1107 22 -0.10319554934487485 1107 23 0.08573937252391062 1107 24 -0.21597374606469089 1107 25 -0.3288964205093734 1108 1 0.3678250011680362 1108 2 0.436726624698089 1108 3 0.4509808682938896 1108 4 0.7812073816619574 1108 5 0.5316947656003919 1108 6 0.6049188100716592 1108 7 0.5258488506969243 1108 8 0.5727035190313359 1108 9 0.8639474547664108 1108 10 0.8663862278285066 1108 11 0.6459654750585742 1108 12 0.572730723302378 1108 13 0.5232156525023015 1108 14 0.6296014155904643 1108 15 0.5471213666538972 1108 16 0.605898708790221 1108 17 0.6925024005694596 1108 18 0.8055838066790528 1108 19 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0.5488607208647664 2213 24 0.3014835854305389 2213 25 0.07234751845953014 2214 1 0.3492353688045391 2214 2 0.22945289408135494 2214 3 0.37833695291649505 2214 4 0.6258660068542667 2214 5 0.43272580672568495 2214 6 0.31751962619489404 2214 7 0.19737786035990013 2214 8 0.16604120039489345 2214 9 0.3412115094849071 2214 10 0.061000454120687664 2214 11 0.12298719003328529 2214 12 0.005174751769381203 2214 13 -0.03799624354507358 2214 14 0.22149757864091413 2214 15 -0.02651796866224032 2214 16 0.13885153844610443 2214 17 0.17205083823850487 2214 18 0.37236346089157024 2214 19 0.19341931674879476 2214 20 0.2513522891174347 2214 21 0.272531957346132 2214 22 0.26862951184565276 2214 23 0.39236259672281903 2214 24 0.23371228194643834 2214 25 0.22514997254783964 2215 1 0.5017643941717971 2215 2 0.22667243577289753 2215 3 0.4610832922409678 2215 4 0.932398102286669 2215 5 0.6287169048236123 2215 6 0.6523200110413754 2215 7 0.5992436853984913 2215 8 0.4844858837104056 2215 9 0.7439563538877962 2215 10 0.7699042453759688 2215 11 0.44259355199240247 2215 12 0.3291319584071104 2215 13 0.30403934955205614 2215 14 0.5970202707154115 2215 15 0.42531136632626954 2215 16 0.4341218228686188 2215 17 0.6929933274280486 2215 18 0.8055643891666904 2215 19 0.5038342040321169 2215 20 -0.3721893580698463 2215 21 0.1986916954665361 2215 22 -0.5330384414301079 2215 23 0.26725514923433735 2215 24 0.35596312184732426 2215 25 0.3632376037008527 2216 1 0.3682715793669118 2216 2 -0.15585658046425266 2216 3 0.40046079877023644 2216 4 0.8020926292141226 2216 5 0.5564745234598902 2216 6 0.5445716355961707 2216 7 0.5579586313026326 2216 8 0.49393939869500514 2216 9 0.7995074858188929 2216 10 0.5697430492409481 2216 11 0.4177772630803176 2216 12 0.07961870516899694 2216 13 0.27486329554708533 2216 14 0.34753806798941156 2216 15 0.3712853076170859 2216 16 0.45460113483191833 2216 17 0.48267754771770655 2216 18 0.745632836222633 2216 19 0.5307635935683876 2216 20 -0.6518453290566768 2216 21 -0.9719509248661922 2216 22 -0.9125795121956912 2216 23 -1.3204897000454139 2216 24 -1.0874948284297474 2216 25 -0.7838377778806205 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6895.0 3 882.0 4 785.0 5 585.0 6 692.0 7 887.0 8 429.0 9 821.0 10 2085.0 11 4181.0 12 7199.0 13 11090.0 14 15067.0 15 18552.0 16 20965.0 17 22423.0 18 22760.0 19 22534.0 20 22702.0 21 22799.0 22 23320.0 23 24426.0 24 25485.0 25 27211.0 26 29650.0 27 33104.0 28 38669.0 29 46995.0 30 57603.0 31 72578.0 32 95028.0 33 126412.0 34 173481.0 35 244576.0 36 362039.0 37 592975.0 38 1188849.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.6169264185415 20.789916586302926 13.977907060448011 23.615249934707556 2 15.929850440385863 21.202952825332673 39.62856145384527 23.238635280436192 3 18.113693881279737 26.317868007989038 30.051292108693133 25.517146002038093 4 13.720639931214043 17.980139962965534 37.32213573956978 30.97708436625064 5 13.652835944968123 37.31107465536499 35.30262940547516 13.73345999419173 6 30.496092453078738 38.65959010545102 17.531000190146816 13.313317251323427 7 26.01327437189045 32.656540455122176 23.167561152329213 18.162624020658164 8 24.471968961883512 36.097401019798276 20.9873650540976 18.443264964220614 9 25.078470534690517 17.19934630490292 21.555604767813357 36.1665783925932 10 15.324778554994003 29.830592967844595 33.53689169893915 21.307736778222257 11 32.05477942107435 25.292702581526395 23.907696020090953 18.744821977308305 12 21.328532414399064 27.17082276389034 30.865396336602004 20.635248485108598 13 26.690954619647318 24.056187468751137 26.489591260389222 22.763266651212323 14 21.373283767776925 23.593721365089063 27.50062950243234 27.53236536470168 15 22.9413153545476 30.64410732896265 24.119503754987374 22.295073561502377 16 22.467573601689324 30.522365134459566 25.520337506879187 21.489723756971927 17 21.208275649900017 29.686458493565095 27.577838352835403 21.52742750369949 18 21.143864427792124 29.422822752122972 28.48640537854394 20.94690744154097 19 21.828487939743273 28.64543080690922 28.6125853958334 20.913495857514103 20 22.685895417152434 28.223049771434138 28.119753435489546 20.97130137592388 21 23.108267368018204 28.380654095353684 27.25965605240584 21.251422484222275 22 22.54679802368621 27.988155702637705 28.129401838553374 21.33564443512271 23 22.781407202823363 28.276967693814477 27.547921914207617 21.39370318915454 24 22.502526132170573 27.769873009525554 27.909387616693003 21.818213241610874 25 22.761564372985944 27.92844681557937 27.57586547500749 21.734123336427196 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 10613.0 1 10613.0 2 8908.0 3 7203.0 4 7203.0 5 7203.0 6 7589.0 7 7975.0 8 7975.0 9 7975.0 10 10631.0 11 13287.0 12 13287.0 13 13287.0 14 20473.0 15 27659.0 16 27659.0 17 27659.0 18 44263.0 19 60867.0 20 60867.0 21 60867.0 22 88626.0 23 116385.0 24 116385.0 25 116385.0 26 152176.5 27 187968.0 28 187968.0 29 187968.0 30 227107.5 31 266247.0 32 266247.0 33 266247.0 34 300483.0 35 334719.0 36 334719.0 37 334719.0 38 359081.0 39 383443.0 40 383443.0 41 383443.0 42 388520.5 43 393598.0 44 393598.0 45 393598.0 46 384624.5 47 375651.0 48 375651.0 49 375651.0 50 362671.0 51 349691.0 52 349691.0 53 349691.0 54 322232.0 55 294773.0 56 294773.0 57 294773.0 58 257089.5 59 219406.0 60 219406.0 61 219406.0 62 185342.0 63 151278.0 64 151278.0 65 151278.0 66 119648.5 67 88019.0 68 88019.0 69 88019.0 70 66366.0 71 44713.0 72 44713.0 73 44713.0 74 32665.0 75 20617.0 76 20617.0 77 20617.0 78 14507.5 79 8398.0 80 8398.0 81 8398.0 82 5702.5 83 3007.0 84 3007.0 85 3007.0 86 1994.5 87 982.0 88 982.0 89 982.0 90 586.0 91 190.0 92 190.0 93 190.0 94 114.0 95 38.0 96 38.0 97 38.0 98 22.5 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14661092916755525 2 0.2874595973427066 3 0.3456465524154863 4 0.3730915480700287 5 0.38304184411361275 6 0.3395575652843379 7 0.3429733385530309 8 0.36085416905523277 9 0.3461217904354784 10 0.37843797579493954 11 0.2969940601187976 12 0.3428545290480329 13 0.315201616759744 14 0.3240232225058469 15 0.3642105375714268 16 0.3146966763635024 17 0.3629036330164486 18 0.3320428640932132 19 0.3452307191479933 20 0.3080433440836134 21 0.34716137360421107 22 0.302429594972457 23 0.28532102625274225 24 0.3211717943858945 25 0.3157065571559856 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3366734.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.47326706722876 #Duplication Level Percentage of deduplicated Percentage of total 1 86.84835624888427 67.28425898019638 2 9.231419058988282 14.303763883330097 3 1.9979979794505769 4.64374293185274 4 0.7208959563934328 2.2340065982941493 5 0.3548335612320315 1.3745057626872523 6 0.19670108903337866 0.9143445601858613 7 0.13463721935464953 0.7301549676576279 8 0.0926308644278691 0.5741132558790851 9 0.06327635280179456 0.44120032016882577 >10 0.3357325235902691 4.479674843654311 >50 0.016423939757220077 0.8631211958331806 >100 0.006018525290086038 0.7898086359748113 >500 5.767932851723998E-4 0.32263335441596863 >1k 4.6143462545926363E-4 0.8897590353232118 >5k 3.8452885454938636E-5 0.15491167454651292 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGGTATCAACGCAGAGTACATGGG 5200 0.1544523564974245 No Hit GTATCAACGCAGAGTACTTTTTTTT 4533 0.13464087153900486 No Hit GGTATCAACGCAGAGTACTTTTTTT 3863 0.1147402794518367 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 3840 0.1140571247980981 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.970237624950471E-5 0.0 5 0.0 0.0 0.0 2.970237624950471E-5 0.0 6 0.0 0.0 0.0 2.970237624950471E-5 0.0 7 0.0 0.0 0.0 5.940475249900942E-5 0.0 8 0.0 0.0 0.0 5.940475249900942E-5 0.0 9 0.0 0.0 0.0 1.1880950499801885E-4 0.0 10 0.0 0.0 0.0 1.1880950499801885E-4 0.0 11 0.0 0.0 0.0 1.1880950499801885E-4 0.0 12 0.0 0.0 0.0 1.4851188124752356E-4 0.0 13 0.0 0.0 0.0 1.4851188124752356E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCGAC 75 1.4814163E-5 12.665315 7 TACGGTC 70 1.08725246E-4 12.219541 5 GTGGTAT 1290 0.0 11.199951 1 TTACGGT 60 0.005859923 11.087937 4 TAGTACG 210 0.0 10.860843 2 GGACCGA 105 3.473955E-6 10.855984 6 AGTACGG 240 0.0 10.691301 3 AACGCCC 80 3.7846636E-4 10.6839695 18 GGTATCA 2850 0.0 10.67253 1 GTACGGG 145 1.4360921E-8 10.487114 4 TGGTATC 1270 0.0 10.476009 2 ATACCGT 115 1.1126014E-4 9.085986 6 TCTATAC 335 0.0 9.077854 3 ATAGCGA 85 0.007445929 8.940089 8 GTCTTAT 310 0.0 8.891982 1 GTATACG 100 0.0028917978 8.5547 1 GTCCTAA 345 0.0 8.540925 1 GTATAGA 450 0.0 8.449086 1 GTCTTGG 530 0.0 8.429159 1 AGTACTG 500 0.0 8.363597 5 >>END_MODULE