FastQCFastQC Report
Wed 25 May 2016
SRR1295189_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295189_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3366734
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT118850.3530127417253635No Hit
GGTATCAACGCAGAGTACTTTTTTT104130.30929084388609257No Hit
TATCAACGCAGAGTACTTTTTTTTT68630.20384740820035083No Hit
GTGGTATCAACGCAGAGTACATGGG56480.1677590210572026No Hit
ACGCAGAGTACTTTTTTTTTTTTTT43960.13057164599282273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACGA957.392373E-813.00057912
GGTATCA38950.012.5876081
AGCGTCA3200.011.876063
GTGGTAT14400.011.4152291
ACGCACG752.0743272E-411.40050711
GTACCGT600.005876770711.0836636
TGGTATC13950.010.9648092
CAGCGTC3600.010.5561832
GCAGCGT3400.010.3400781
GTCTAAC2106.730261E-119.5016941
TGTCACG1106.838828E-59.5002827
GCACCGT1002.7561604E-49.5002816
ACGATTT1304.251031E-69.49844815
TAACCCG1252.7511694E-59.1213555
GTCACGC1054.4929123E-49.0480218
TACCCCG950.00182214439.0013375
CTATACT4500.08.8674564
CTTGTAC3800.08.750783
TACACCG1107.1372266E-48.6376475
GCTATAC2106.375558E-98.59600453
Produced by FastQC (version 0.11.5)