FastQCFastQC Report
Wed 25 May 2016
SRR1295188_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295188_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2857997
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG43090.15076992733022462No Hit
GTATCAACGCAGAGTACTTTTTTTT40020.14002813858796911No Hit
GGTATCAACGCAGAGTACTTTTTTT34660.12127374521386833No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN32340.11315617196239185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10500.011.315581
ACACCGT1104.977119E-711.2265776
GTGACGA855.3343683E-511.17479611
GGTATCA22950.011.0996231
AAAACCG600.00585968111.0879055
TGGTATC10200.010.9943432
GTCTACG803.754132E-410.6932231
GTCCTAG2450.010.0870321
TCGGGAC800.00450205579.5039193
GTTAGAC1401.0533913E-69.5039193
ACGCTTT800.0045197059.4994126
GTCTAGG2950.09.3439851
GTTCTAG2850.09.3383311
GACTGTG5350.09.2330727
TACGATG950.00182545658.9994437
GCGTCAG1401.0783637E-58.8261531
GTCTATA2502.5465852E-118.744681
GTATAGA4250.08.7223161
TAGGACG1107.117673E-48.6399264
CTAGAAC2855.456968E-128.3367713
Produced by FastQC (version 0.11.5)