##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295188_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2857997
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.966777431886737	31.0	30.0	33.0	26.0	34.0
2	30.24060592085996	31.0	30.0	34.0	26.0	34.0
3	30.250157015560198	31.0	30.0	34.0	26.0	34.0
4	33.66191532041496	35.0	35.0	37.0	28.0	37.0
5	33.73942379925521	35.0	35.0	37.0	28.0	37.0
6	34.01511828039008	36.0	35.0	37.0	29.0	37.0
7	33.948089168743	36.0	35.0	37.0	28.0	37.0
8	34.1116736651578	37.0	35.0	37.0	29.0	37.0
9	35.11322720072834	39.0	35.0	39.0	27.0	39.0
10	35.17481124017975	39.0	35.0	39.0	27.0	39.0
11	35.54487006109524	39.0	35.0	39.0	28.0	39.0
12	35.54674060189706	39.0	35.0	39.0	28.0	39.0
13	35.4655638196961	39.0	35.0	39.0	28.0	39.0
14	36.124055063738695	39.0	36.0	41.0	27.0	41.0
15	36.4699473092519	40.0	36.0	41.0	27.0	41.0
16	36.449175768903885	40.0	36.0	41.0	27.0	41.0
17	36.13138362286595	39.0	36.0	41.0	27.0	41.0
18	36.03681214500925	39.0	36.0	41.0	27.0	41.0
19	36.468109308722156	40.0	36.0	41.0	27.0	41.0
20	36.4864812664254	40.0	36.0	41.0	27.0	41.0
21	36.52593687117236	40.0	36.0	41.0	27.0	41.0
22	36.43503124740859	40.0	36.0	41.0	27.0	41.0
23	36.15109533005108	39.0	36.0	41.0	27.0	41.0
24	36.4287383786617	40.0	36.0	41.0	27.0	41.0
25	35.14643612292105	39.0	34.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.337388712032201
1101	2	0.030924373774769975
1101	3	-0.2822030504111872
1101	4	-0.2640896545197009
1101	5	-0.4702016378938083
1101	6	-0.5552296593957067
1101	7	-0.6114488880352695
1101	8	-0.4322139149362414
1101	9	-0.2906167577817129
1101	10	-0.15806826626860726
1101	11	-0.20746513656780508
1101	12	-0.14159173548690518
1101	13	-0.1544968064928085
1101	14	0.11164907260699408
1101	15	0.01216477046163078
1101	16	-0.11059715500984879
1101	17	-0.12216445378025043
1101	18	0.09252372447876667
1101	19	-0.11769877840020371
1101	20	-0.10767771025686557
1101	21	-0.12431275386716578
1101	22	-0.09699202206841306
1101	23	0.03531188962008258
1101	24	-0.25208032744293263
1101	25	-0.19670917391069054
1102	1	-0.4230853500766685
1102	2	-0.008334121901679481
1102	3	-0.21420867012593447
1102	4	-0.2318805506964381
1102	5	-0.8330152361863199
1102	6	-0.5516994406639668
1102	7	-0.5226912612888484
1102	8	-0.539530303157143
1102	9	-0.38238285447926046
1102	10	0.005735477203344885
1102	11	-0.24617624931144633
1102	12	-0.269063132175674
1102	13	-0.3648187209054825
1102	14	-0.0935910795410777
1102	15	-0.13731828716128547
1102	16	-0.29006518903970857
1102	17	-0.44275657143475655
1102	18	-0.017599916018923523
1102	19	-0.17008873325126928
1102	20	-0.26900765383827974
1102	21	-0.15562914313009912
1102	22	-0.2663820829469188
1102	23	-0.3287907626549895
1102	24	-0.36922975761709154
1102	25	-0.44726029515737054
1103	1	-0.40991256922342956
1103	2	-0.14842057795179642
1103	3	-0.45633881726416803
1103	4	-0.3150339742034305
1103	5	-0.5282538552650706
1103	6	-0.5489114988175885
1103	7	-0.46667494988728464
1103	8	-0.2980254813624512
1103	9	0.035317778528579424
1103	10	0.09950886415984428
1103	11	-0.08662074969261369
1103	12	-0.25854017737261614
1103	13	-0.27626831962995624
1103	14	-0.25590715146442733
1103	15	-0.2699099270448997
1103	16	-0.297271145581945
1103	17	-0.12234176753487702
1103	18	0.15347187312696775
1103	19	-0.02206859729967192
1103	20	-0.051295047976800845
1103	21	-0.10457934683768855
1103	22	-0.08758632376761
1103	23	-2.32197638077443
1103	24	-1.0329703138042277
1103	25	-0.5961193762551176
1104	1	-0.2621370054532015
1104	2	-0.02757206730485251
1104	3	-0.24881882904210428
1104	4	-0.40793750950771823
1104	5	-0.31151160199149075
1104	6	-0.39100065310874044
1104	7	-0.346896246631772
1104	8	-0.2018074564104353
1104	9	-2.5659141916443673
1104	10	-0.789853951337129
1104	11	-0.4196784250336876
1104	12	-0.24149487145655968
1104	13	-0.1002603042356256
1104	14	0.1420407683617384
1104	15	-0.15979299948590153
1104	16	-0.027936136213433826
1104	17	0.19284606003080285
1104	18	0.3863077717857166
1104	19	0.0056084127481028645
1104	20	-0.06937913827516695
1104	21	-0.05308158406145225
1104	22	-0.09975409922761003
1104	23	-0.043088284335453864
1104	24	-0.05988834106945262
1104	25	-0.17679211723417865
1105	1	-0.41945903423106756
1105	2	-0.31220162302707166
1105	3	-0.31249706942970334
1105	4	-0.13109204278023157
1105	5	-0.31778913818548915
1105	6	-0.32562907230678917
1105	7	-0.3777826210288424
1105	8	-0.38053703303073405
1105	9	-2.127699057989396
1105	10	-0.5894638897923343
1105	11	-0.460505705009993
1105	12	-0.30605261497191094
1105	13	-0.3366319150762038
1105	14	-0.26165804553154004
1105	15	-0.20473406708028108
1105	16	-0.3389007034736551
1105	17	-1.5070903322309235
1105	18	-0.3256299395963609
1105	19	-0.3627893154717228
1105	20	-0.3827145540718604
1105	21	-0.22958368077391356
1105	22	-0.08811442586089413
1105	23	-0.08840572144818992
1105	24	-0.1537666349677238
1105	25	-0.6958998291498588
1106	1	-0.41696633285285856
1106	2	-0.5696289991521084
1106	3	-0.5855240985773307
1106	4	-0.3639837354829183
1106	5	-0.44756522660370024
1106	6	-0.46205603323586786
1106	7	-0.49808390508499656
1106	8	-0.6135359574443626
1106	9	-3.2186361258341556
1106	10	-0.9800207972472279
1106	11	-0.8480267236240664
1106	12	-0.4517659074540319
1106	13	-0.5256027957820137
1106	14	-0.7875649108674736
1106	15	-0.737936284945917
1106	16	-0.758959462964043
1106	17	-0.6202163000679448
1106	18	-0.27681406538003017
1106	19	-0.4280153466234111
1106	20	-0.6858854088415498
1106	21	-0.39436171440151213
1106	22	-0.473720366759224
1106	23	-0.28760742277082585
1106	24	-0.4505372059656878
1106	25	-0.7809885731984423
1107	1	-0.4137312894362246
1107	2	-0.15909366643209566
1107	3	-0.302716841805001
1107	4	-1.5056732080719755
1107	5	-0.49160841825689516
1107	6	-0.4261924483878019
1107	7	-0.25146999042684826
1107	8	-0.12605638243197603
1107	9	0.17409632821688348
1107	10	0.3911618293401702
1107	11	0.05068984250641506
1107	12	-0.5467590260684148
1107	13	-0.18366273090792617
1107	14	-0.009860653069537761
1107	15	-0.7351800866817371
1107	16	-1.1089194843845362
1107	17	-0.15917043507830186
1107	18	-0.042891369271103486
1107	19	-0.0032095622457930517
1107	20	-0.416893259852948
1107	21	-0.20484185872577143
1107	22	-0.04905157635154467
1107	23	0.23681304626188648
1107	24	-0.16689446553704812
1107	25	-0.3247582522185084
1108	1	0.2645742514653371
1108	2	0.19410049251216321
1108	3	0.36065360895740994
1108	4	0.6899993965337714
1108	5	0.5755458137127221
1108	6	0.4486988059142405
1108	7	0.3723204796804467
1108	8	0.318784414255326
1108	9	0.6840411545190079
1108	10	0.7820809567830693
1108	11	0.5707810570797349
1108	12	0.4377850036942732
1108	13	0.5011957627646169
1108	14	0.499913497327789
1108	15	0.4804266270050306
1108	16	0.5009731809485629
1108	17	0.684405949368859
1108	18	0.6871658395022564
1108	19	0.35868255015380157
1108	20	0.5694504303478638
1108	21	0.48915166269120647
1108	22	0.49913953630262853
1108	23	0.5725071611874242
1108	24	0.45056922534082844
1108	25	0.5399359801227135
1109	1	0.4347449808463253
1109	2	0.35290912215246095
1109	3	0.537738697376124
1109	4	0.8082837487915668
1109	5	0.7332733655735879
1109	6	0.6109312792185406
1109	7	0.677895719339169
1109	8	0.5696212875165827
1109	9	0.9339164328463809
1109	10	0.7233112777046955
1109	11	0.5939885912178582
1109	12	0.5765191057334675
1109	13	0.4953025756360603
1109	14	0.9514606427778318
1109	15	0.6135293892999485
1109	16	0.7623364318216659
1109	17	1.0861305336573537
1109	18	0.8356589601940243
1109	19	0.6428183480694543
1109	20	0.613977359563556
1109	21	0.6289347517573773
1109	22	0.7172511717140893
1109	23	0.9188452680397603
1109	24	0.6729572001888187
1109	25	0.7349427905979269
1110	1	0.31970654574550394
1110	2	0.20763654424361278
1110	3	0.3080827518721847
1110	4	0.4450064430587162
1110	5	0.4087882852274518
1110	6	0.4333975608031082
1110	7	0.4094433685982892
1110	8	0.40666481973806157
1110	9	0.8706106260468687
1110	10	0.8170055143113188
1110	11	0.5007287834404437
1110	12	0.41005578954594313
1110	13	0.4761001599582215
1110	14	0.6608680847026065
1110	15	0.4405512023765894
1110	16	0.47139928873092174
1110	17	0.8267893836978786
1110	18	0.9181417820430653
1110	19	0.47489433499065115
1110	20	0.5249399354352988
1110	21	0.5109468581653402
1110	22	0.5054436919410037
1110	23	0.6881961258162264
1110	24	0.5472167408692101
1110	25	0.5357423362344278
1111	1	0.23504653730925895
1111	2	0.19315434125106634
1111	3	0.3201385387886617
1111	4	0.6180654807819863
1111	5	0.5511984159003873
1111	6	0.38913114054692244
1111	7	0.2833020176920016
1111	8	0.28516990564079236
1111	9	0.7391883569739264
1111	10	0.5238510850292428
1111	11	0.44530799316014225
1111	12	0.26048465177547087
1111	13	0.22049383826166036
1111	14	0.3170607486876449
1111	15	0.342888424707418
1111	16	0.21424868840989575
1111	17	0.49039954919287254
1111	18	0.576561656481239
1111	19	0.33860000011922153
1111	20	-0.1443626566694789
1111	21	-0.2776266889856913
1111	22	-0.10157559065955724
1111	23	0.2565391558839565
1111	24	0.26962704830851436
1111	25	0.1638150428980083
1112	1	0.24108983720969945
1112	2	0.13030922701632974
1112	3	0.25261793309980973
1112	4	0.5918158846481276
1112	5	0.6017930676362937
1112	6	0.5145689895783292
1112	7	0.3950625089240276
1112	8	0.3644647365084168
1112	9	-1.205816451269932
1112	10	0.20848315286840347
1112	11	0.10777349820014592
1112	12	0.12812169626283065
1112	13	0.1099563677040365
1112	14	0.4426008756557991
1112	15	0.2497331167504555
1112	16	0.23806636772351197
1112	17	0.2614318578484287
1112	18	0.434218881332022
1112	19	0.26896525679460836
1112	20	0.3149084250069052
1112	21	0.22866799119002224
1112	22	0.2470706391313584
1112	23	0.039886645537393406
1112	24	0.18386450043612967
1112	25	0.25065196327118144
1113	1	0.30181566768820645
1113	2	0.26361148460088657
1113	3	0.23339066350397175
1113	4	0.501559384463377
1113	5	0.7211862137337661
1113	6	0.45198863476008455
1113	7	0.3964848954520619
1113	8	0.26281044496838746
1113	9	0.24587295705914158
1113	10	0.4174787769138746
1113	11	0.3127329729782389
1113	12	0.1603399410675621
1113	13	0.15218294212165517
1113	14	-0.10753775737509841
1113	15	0.15653933201950565
1113	16	0.18994187266714846
1113	17	0.6583835416201822
1113	18	0.3748401935099821
1113	19	0.1776563265727873
1113	20	0.2668915617906009
1113	21	0.1938104848309763
1113	22	0.30824059633808076
1113	23	0.02029264035353151
1113	24	0.08209658259777797
1113	25	0.15351489864108459
1114	1	0.4357195222181147
1114	2	0.28697349271298833
1114	3	0.3835885185940029
1114	4	0.4897058369865448
1114	5	0.5691862384711683
1114	6	0.441214168359231
1114	7	0.27845538902519706
1114	8	0.34495533726789773
1114	9	0.05021437216257141
1114	10	0.30193060920484527
1114	11	0.285975490574927
1114	12	0.27707373740270924
1114	13	0.2725086984458187
1114	14	0.7051717044184187
1114	15	0.306335911012674
1114	16	0.1715321169402202
1114	17	0.5233879722666472
1114	18	0.35859875994184875
1114	19	0.32321335557475095
1114	20	0.2668941238792826
1114	21	0.44426764096554194
1114	22	0.12818398301292433
1114	23	0.624467784736062
1114	24	0.3534353799533534
1114	25	0.2625878731111939
1115	1	0.540699514524043
1115	2	0.4109732376265569
1115	3	0.5250564995433997
1115	4	0.7390213723379659
1115	5	0.7599947795115725
1115	6	0.6092464806900963
1115	7	0.543535819022118
1115	8	0.4911847588265772
1115	9	0.6280337944712926
1115	10	0.372690307069135
1115	11	0.48558650104556733
1115	12	0.4733012233461693
1115	13	0.37931568684629724
1115	14	0.5937004018150489
1115	15	0.44531178737110366
1115	16	0.5200569189112301
1115	17	0.6969137161218484
1115	18	0.9199130876076325
1115	19	0.5657996036507953
1115	20	0.625951956259847
1115	21	0.40772296644787787
1115	22	-0.11196943868815623
1115	23	0.42663391098587766
1115	24	0.5002495474124089
1115	25	0.5500515327996212
1116	1	0.7029013891100249
1116	2	0.4252256898827227
1116	3	0.7252528716700049
1116	4	0.8696617945937177
1116	5	1.03497104549416
1116	6	0.7365304752352912
1116	7	0.6656382101369687
1116	8	0.46201159864668995
1116	9	0.7962660258107661
1116	10	0.5997221746164882
1116	11	0.36381558338720055
1116	12	0.39875931707339873
1116	13	0.38487602459703396
1116	14	0.8817512239469139
1116	15	0.5002847179020407
1116	16	0.6127858414722951
1116	17	0.9904965194860935
1116	18	0.9624500663203079
1116	19	0.6528135871693195
1116	20	0.5113817815163628
1116	21	0.65806997772264
1116	22	0.700770458751883
1116	23	0.8977358996712397
1116	24	0.6744955618325434
1116	25	0.5385464257777173
1201	1	-0.4562207466378112
1201	2	-0.04851059814417269
1201	3	-0.38874161037687927
1201	4	-0.18922951198704396
1201	5	-0.6593028640482572
1201	6	-0.5945706356184388
1201	7	-0.6422516262025297
1201	8	-0.5701152429332765
1201	9	-0.0785154901493712
1201	10	0.08511309361684027
1201	11	-0.08358443325920462
1201	12	-0.020028942255784443
1201	13	-0.17937973578186472
1201	14	0.2219011303932703
1201	15	-0.09662105672371268
1201	16	-0.01466493667541613
1201	17	0.0758667809019542
1201	18	0.22812895090243046
1201	19	-0.12325676436919508
1201	20	0.006713267700632741
1201	21	-0.2061877739213358
1201	22	-0.224076332023607
1201	23	-0.24624502888002553
1201	24	-0.36986466690221675
1201	25	-0.39349685429715464
1202	1	-0.5153812649909426
1202	2	-0.36399191348840887
1202	3	-0.6148336283532991
1202	4	-0.20426284311761833
1202	5	-0.735437624537262
1202	6	-0.6925421900180666
1202	7	-0.6909173958125905
1202	8	-0.3988234418098031
1202	9	0.16604304701976247
1202	10	0.2212415120604092
1202	11	-0.07024743816850076
1202	12	-0.16176272309263595
1202	13	-0.017959836011115726
1202	14	-0.06423847880763134
1202	15	-0.1702271867548717
1202	16	-0.12129634651380883
1202	17	0.25729433909138066
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2211	9	-1.2710801061552388
2211	10	0.12239983214548289
2211	11	0.1822276611892093
2211	12	0.015633691070355837
2211	13	0.16182753977984277
2211	14	0.39827505610048064
2211	15	0.2807957195559041
2211	16	0.4404407288543126
2211	17	0.5663453909802314
2211	18	0.6208554017277308
2211	19	0.4421163606301093
2211	20	0.5288352970179346
2211	21	0.3398633805305309
2211	22	0.4204689100535077
2211	23	-1.738398347778265
2211	24	-0.3436829067390761
2211	25	0.09288208871519288
2212	1	0.17757328802020567
2212	2	-1.3076607006468528
2212	3	-0.16932307197089713
2212	4	0.4819948230913056
2212	5	0.6206842165767199
2212	6	0.5334343526958065
2212	7	0.5187381414730154
2212	8	0.41975560817541435
2212	9	0.875374004335221
2212	10	0.7336432904130135
2212	11	0.4483170026755019
2212	12	0.39270471675345675
2212	13	0.335838659972687
2212	14	0.7501779638903372
2212	15	0.550612716675829
2212	16	0.5888627704495235
2212	17	0.8018010804968796
2212	18	1.0041303146142369
2212	19	0.6511521069349868
2212	20	0.5771350763234224
2212	21	0.501654914744158
2212	22	0.5915085612794044
2212	23	0.7388348873234776
2212	24	0.5192978615271429
2212	25	0.6189425300587388
2213	1	0.3642842111027065
2213	2	0.1642354985494343
2213	3	0.3104033555523813
2213	4	0.5433875547401215
2213	5	0.6047225516048442
2213	6	0.4008202851146194
2213	7	0.32557381038075306
2213	8	0.317071334329448
2213	9	0.640615074824133
2213	10	0.6740640123485448
2213	11	0.3427614383204016
2213	12	0.42381721291246066
2213	13	0.20544322088082367
2213	14	-1.428970412928571
2213	15	-0.4112245062309938
2213	16	0.0370279251789114
2213	17	0.4483254638513827
2213	18	0.23957996416368132
2213	19	0.1659382411096999
2213	20	0.22090256321298085
2213	21	0.38320595367086696
2213	22	0.28359178065866786
2213	23	0.49789705151096086
2213	24	0.2201797868105828
2213	25	0.05072245386878649
2214	1	0.547657788569655
2214	2	0.40556702916261145
2214	3	0.3798187211305297
2214	4	0.5593199757939686
2214	5	0.5789273081952331
2214	6	0.45774386144554313
2214	7	0.28869413550791023
2214	8	0.23448883767054696
2214	9	0.3916194402147539
2214	10	0.2506430371773192
2214	11	0.22473917933842813
2214	12	-5.456462369721748E-4
2214	13	-0.016737502492851775
2214	14	0.23557875273686335
2214	15	0.082919154220086
2214	16	0.2844869578886531
2214	17	0.2815693987459227
2214	18	0.4846629652518928
2214	19	0.17036814781656062
2214	20	0.26013525247062574
2214	21	0.35711746303528
2214	22	0.38594824550952467
2214	23	0.380030360224751
2214	24	0.37209507524072194
2214	25	0.25211000750361023
2215	1	0.48782099206138696
2215	2	0.19481772314273726
2215	3	0.26576410260548755
2215	4	0.6121880937781157
2215	5	0.5168736255900441
2215	6	0.5094517053769607
2215	7	0.47577900927102945
2215	8	0.29951650925647044
2215	9	0.5870182538771047
2215	10	0.5835566627832165
2215	11	0.32593687043995345
2215	12	0.18964291742470607
2215	13	0.2435691544098404
2215	14	0.4154129626312084
2215	15	0.26335201221374405
2215	16	0.378339825463641
2215	17	0.4456800564591532
2215	18	0.6309294422664493
2215	19	0.29747159188806904
2215	20	-0.49723159923272675
2215	21	-0.06607244403667067
2215	22	-0.8793577585693271
2215	23	0.14964339735129784
2215	24	0.13846164039266284
2215	25	0.25444079840277567
2216	1	0.28299509450793536
2216	2	-0.27001270553063605
2216	3	0.31640208050937346
2216	4	0.4491549134358479
2216	5	0.5640746684710223
2216	6	0.5308557393067659
2216	7	0.4651003384247474
2216	8	0.31743892993988254
2216	9	0.6922285650027717
2216	10	0.3288611541243185
2216	11	0.28826474316546324
2216	12	-0.07687871559026149
2216	13	0.13553334639615855
2216	14	0.346330194639755
2216	15	0.19939178545059377
2216	16	0.23682823169070844
2216	17	0.3685393427436452
2216	18	0.6088637020625498
2216	19	0.3795224300342781
2216	20	-0.8417321456058886
2216	21	-1.013369608806748
2216	22	-1.0490470077556822
2216	23	-1.4476619919933214
2216	24	-1.118287545760822
2216	25	-0.8746332739815372
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5653.0
3	823.0
4	728.0
5	491.0
6	602.0
7	801.0
8	296.0
9	466.0
10	1091.0
11	2518.0
12	4865.0
13	7949.0
14	11605.0
15	14684.0
16	16946.0
17	18095.0
18	18934.0
19	19340.0
20	19400.0
21	19687.0
22	19818.0
23	20909.0
24	21895.0
25	23554.0
26	25506.0
27	29160.0
28	33936.0
29	40444.0
30	49875.0
31	63361.0
32	82148.0
33	110451.0
34	150873.0
35	214004.0
36	315878.0
37	513818.0
38	977393.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.30129743984532	20.864894488184564	13.632701917514265	23.201106154455857
2	15.965040814722494	21.093021917562123	39.877724256408534	23.064213011306858
3	18.058689159800824	26.117511739166154	30.33648414168034	25.487314959352673
4	12.910539992561418	17.66519147674811	37.93515088063605	31.48911765005442
5	13.265515069795116	37.64468573319205	35.09519623169948	13.994602965313357
6	30.62078931670939	38.45697427307399	17.778602452514008	13.143633957702614
7	25.715050118374794	32.92760897661189	23.756204450808013	17.6011364542053
8	23.57990719417235	37.21823416088825	20.932511045888106	18.269347599051294
9	24.668998746884327	16.850526017160206	21.69003135773626	36.79044387821921
10	14.749498448985095	30.100893238474164	34.148704958752	21.000903353788747
11	32.01219854571998	25.32338779864679	24.022010724613267	18.642402931019962
12	20.987084757485906	27.459136256949602	31.2481808590383	20.305598126526192
13	26.48093334189765	24.280500984369773	26.734528336125514	22.504037337607066
14	21.03588907587332	23.60198713668796	27.861897370118953	27.500226417319766
15	22.890998888890447	31.064256125500595	24.046637102630843	21.99810788297811
16	22.292569751810266	30.94558498857506	25.402119317802903	21.359725941811774
17	20.745548868943377	30.129261105748007	27.806641764235522	21.31854826107309
18	20.693792838095344	29.868535054744292	28.6862352186191	20.75143688854126
19	21.306676741127323	28.86775374404082	28.983619404478596	20.841950110353263
20	22.47727206521583	28.47176815488443	28.310006117405756	20.740953662493986
21	22.8688460698483	28.705524957708022	27.41043195047292	21.01519702197076
22	22.149863883576852	28.230268909338424	28.606453501247103	21.013413705837618
23	22.390606534413887	28.641782015905143	27.84357744984378	21.124033999837184
24	21.97186292380861	28.004706478674297	28.2855928327294	21.73783776478769
25	22.424260080462584	28.203287720083985	27.850886583695623	21.521565615757808
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8588.0
1	8588.0
2	7371.5
3	6155.0
4	6155.0
5	6155.0
6	6422.0
7	6689.0
8	6689.0
9	6689.0
10	9154.0
11	11619.0
12	11619.0
13	11619.0
14	18085.0
15	24551.0
16	24551.0
17	24551.0
18	39236.5
19	53922.0
20	53922.0
21	53922.0
22	77949.5
23	101977.0
24	101977.0
25	101977.0
26	133120.0
27	164263.0
28	164263.0
29	164263.0
30	198720.0
31	233177.0
32	233177.0
33	233177.0
34	262381.0
35	291585.0
36	291585.0
37	291585.0
38	311825.5
39	332066.0
40	332066.0
41	332066.0
42	335682.0
43	339298.0
44	339298.0
45	339298.0
46	329637.0
47	319976.0
48	319976.0
49	319976.0
50	307327.5
51	294679.0
52	294679.0
53	294679.0
54	270372.0
55	246065.0
56	246065.0
57	246065.0
58	213082.0
59	180099.0
60	180099.0
61	180099.0
62	150045.0
63	119991.0
64	119991.0
65	119991.0
66	93698.5
67	67406.0
68	67406.0
69	67406.0
70	50374.0
71	33342.0
72	33342.0
73	33342.0
74	23797.0
75	14252.0
76	14252.0
77	14252.0
78	9990.5
79	5729.0
80	5729.0
81	5729.0
82	3803.0
83	1877.0
84	1877.0
85	1877.0
86	1204.5
87	532.0
88	532.0
89	532.0
90	330.5
91	129.0
92	129.0
93	129.0
94	76.0
95	23.0
96	23.0
97	23.0
98	15.0
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14506663233026487
2	0.2814908483108975
3	0.3442620828503319
4	0.3736882858869341
5	0.3806162147825907
6	0.3406581602429954
7	0.34429707239020896
8	0.3614769364698423
9	0.346256486623324
10	0.3789017273286151
11	0.2966063295377847
12	0.3433173652736514
13	0.31371621453766396
14	0.32452798235967356
15	0.36364628794221965
16	0.31375120407754103
17	0.36214173772750635
18	0.33173582757434666
19	0.3453817481263976
20	0.30615847392422035
21	0.3477610368380373
22	0.3008400638629082
23	0.28345026254401245
24	0.32043420619405827
25	0.3138911622370492
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2857997.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.09820385759585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.2585326571496	68.14734671772754
2	8.923658483323084	13.938433987722615
3	1.9162732025289868	4.4897248565387065
4	0.7171942611231181	2.2404633444276443
5	0.37743189390772347	1.4738376496381937
6	0.20361976875482696	0.9541402925790601
7	0.12806309183156886	0.7001048216747118
8	0.08668049311251347	0.5415672657262407
9	0.06525096922449505	0.4586385146760294
>10	0.30011441802672284	4.04723208918
>50	0.016503944213224847	0.8923507480621243
>100	0.0056453651463424055	0.8128575961091536
>500	4.4845724261956927E-4	0.23189195182507358
>1k	5.829944152424257E-4	1.0714101641128815
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	4309	0.15076992733022462	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4002	0.14002813858796911	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3466	0.12127374521386833	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	3234	0.11315617196239185	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	3.4989539877053754E-5	0.0	0.0	0.0
4	0.0	3.4989539877053754E-5	0.0	0.0	0.0
5	0.0	3.4989539877053754E-5	0.0	0.0	0.0
6	0.0	3.4989539877053754E-5	0.0	0.0	0.0
7	0.0	3.4989539877053754E-5	0.0	0.0	0.0
8	0.0	3.4989539877053754E-5	0.0	0.0	0.0
9	0.0	3.4989539877053754E-5	0.0	6.997907975410751E-5	0.0
10	0.0	3.4989539877053754E-5	0.0	6.997907975410751E-5	0.0
11	0.0	6.997907975410751E-5	0.0	1.0496861963116127E-4	0.0
12	0.0	6.997907975410751E-5	0.0	1.0496861963116127E-4	0.0
13	0.0	6.997907975410751E-5	0.0	1.0496861963116127E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGGTAT	1050	0.0	11.31558	1
ACACCGT	110	4.977119E-7	11.226577	6
GTGACGA	85	5.3343683E-5	11.174796	11
GGTATCA	2295	0.0	11.099623	1
AAAACCG	60	0.005859681	11.087905	5
TGGTATC	1020	0.0	10.994343	2
GTCTACG	80	3.754132E-4	10.693223	1
GTCCTAG	245	0.0	10.087032	1
TCGGGAC	80	0.0045020557	9.503919	3
GTTAGAC	140	1.0533913E-6	9.503919	3
ACGCTTT	80	0.004519705	9.499412	6
GTCTAGG	295	0.0	9.343985	1
GTTCTAG	285	0.0	9.338331	1
GACTGTG	535	0.0	9.233072	7
TACGATG	95	0.0018254565	8.999443	7
GCGTCAG	140	1.0783637E-5	8.826153	1
GTCTATA	250	2.5465852E-11	8.74468	1
GTATAGA	425	0.0	8.722316	1
TAGGACG	110	7.117673E-4	8.639926	4
CTAGAAC	285	5.456968E-12	8.336771	3
>>END_MODULE