FastQCFastQC Report
Wed 25 May 2016
SRR1295188_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295188_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2857997
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT110760.38754414367824735No Hit
GGTATCAACGCAGAGTACTTTTTTT100030.3500003673901687No Hit
TATCAACGCAGAGTACTTTTTTTTT68790.24069304481425277No Hit
GTGGTATCAACGCAGAGTACATGGG48570.1699441951828501No Hit
ACGCAGAGTACTTTTTTTTTTTTTT42140.14744592104190452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT12750.012.7451321
GGTATCA34150.012.2995721
TGGTATC12950.012.1786162
AGCGTCA2800.011.1973573
TGTGCGG750.002648450410.13346410
GCAGCGT2950.09.986151
GACTGCG800.00451562279.5004557
CAGCGTC3350.09.3589842
GTCCTAT1752.658453E-89.2314371
GTACTAG1651.0614167E-79.2149821
GTCTTAG3200.09.2059821
TAACACT4750.09.2007634
TGGACCG1252.7517057E-59.1210765
TTATACT5000.09.1207564
AGCACCG1054.489091E-49.0486855
ACACCGT950.00182508988.9996446
ATACTCG850.00744036268.9408236
CTAACAC3950.08.8994723
CTAGGAC2800.08.822163
GTCCTGG6050.08.7961191
Produced by FastQC (version 0.11.5)