FastQCFastQC Report
Wed 25 May 2016
SRR1295187_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295187_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5849092
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68710.11747122459349246No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN66620.11389802041068939No Hit
GTGGTATCAACGCAGAGTACATGGG65180.1114360998254088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36900.010.5622251
GTGGTAT18050.010.2169781
TGGTATC17450.010.18633652
CCTCGCG800.0045251329.4983359
GTCCTAG5700.09.1724591
TATACCG1252.7393971E-59.1249795
TAGACTG6650.08.8619795
ACGGGTC1951.6369086E-88.7740195
GCACCGT1856.601476E-88.7284996
TGTACCG1651.0432814E-68.6410795
TTAGACT5200.08.5915964
GTGTTAG8100.08.5671391
GTATCAA67050.08.5632011
TAGACCG1000.0028925558.5546675
GTCCTAA5000.08.3652831
TGTCGCC3550.08.29432110
CTAGTAC3800.08.2547873
CACGGGT2201.4517354E-88.2093774
GTGCACG2803.274181E-118.1480031
GTGTAGG7700.08.1480031
Produced by FastQC (version 0.11.5)