FastQCFastQC Report
Wed 25 May 2016
SRR1295187_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295187_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5849092
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT183480.31368971457450145No Hit
GGTATCAACGCAGAGTACTTTTTTT143350.24508077493053623No Hit
TATCAACGCAGAGTACTTTTTTTTT101020.17271056772572563No Hit
GTGGTATCAACGCAGAGTACATGGG73940.1264127833858657No Hit
ACGCAGAGTACTTTTTTTTTTTTTT66430.1135731836667982No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGCG508.718701E-515.200379
TCGACCG1251.4133548E-912.16029559
GGTATCA49500.011.5363991
GTGGTAT19550.011.4700741
AGCGTCA4250.011.1768373
ATCGACC1451.2205419E-911.1382028
TACATCG2200.010.36385
TGGTATC20400.010.1986032
GTGCGAC750.00264872110.13366711
CGCCTAA1606.652044E-89.49958114
CAGCGCA2501.8189894E-129.1202219
CAGCGTC5350.09.056142
CGAGCGC850.0074384048.94139410
GTATTAG8550.08.8904731
GTATCAA90050.08.6628431
ATAACGC1107.147429E-48.6366473
CGTGTCG1107.1480375E-48.6365749
TAGGACT6300.08.5953734
ACCGATC1000.0029062468.5502088
TGAACCG1000.0029064718.5501355
Produced by FastQC (version 0.11.5)