FastQCFastQC Report
Wed 25 May 2016
SRR1295186_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295186_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608378
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA183163.010628260719487No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA181282.9797264200875118No Hit
GCTTATGTACTCTGCGTTGATACCA105451.7332973907669247No Hit
CTTATGTACTCTGCGTTGATACCAC74711.22801942213558No Hit
GAGTACATAAGCAGTGGTATCAACG50740.8340209540778922No Hit
TATGTACTCTGCGTTGATACCACTG43440.7140297643899023No Hit
GTATCAACGCAGAGTACTTTTTTTT39660.6518973401405047No Hit
GCGTTGATACCACTGCTTATGTACT35920.5904224018620002No Hit
CCCATGTACTCTGCGTTGATACCAC34180.5618217621281506No Hit
CATAAGCAGTGGTATCAACGCAGAG31190.5126746858038916No Hit
GGTATCAACGCAGAGTACTTTTTTT29910.49163513473531256No Hit
CTGCTTATGTACTCTGCGTTGATAC28340.46582881037775853No Hit
ACGCAGAGTACATAAGCAGTGGTAT26750.43969374303475794No Hit
TATCAACGCAGAGTACTTTTTTTTT25360.4168461055462229No Hit
GTACTGGTTCACTATCGGTCAGTCA24660.4053401010555937No Hit
ATACAGGGTGACAGCCCCGTACACA24600.4043538720992541No Hit
CATGTACTCTGCGTTGATACCACTG23850.39202601014500854No Hit
ACTCTGCGTTGATACCACTGCTTAT23030.3785475477417No Hit
GTATCAACGCAGAGTACATAAGCAG22430.3686852581783036No Hit
GTACATGGGAAGCAGTGGTATCAAC22000.36161728399120285No Hit
GGATACCACGTGTCCCGCCCTACTC21100.3468238496461082No Hit
GTGGTATCAACGCAGAGTACATGGG20240.3326879012719066No Hit
TATCAACGCAGAGTACATAAGCAGT19920.32742801350476186No Hit
GGTATCAACGCAGAGTACATAAGCA19730.3243049551430196No Hit
ACCCTGTATCGCGCGCCTTTCCAGA17670.2904444276420252No Hit
AAGCAGTGGTATCAACGCAGAGTAC15510.254940185213798No Hit
GTGGTATCAACGCAGAGTACATAAG14980.24622849609946448No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14050.2309419472762No Hit
ATCATTAACTGAATCCATAGGTTAA13580.2232164871182061No Hit
ACATAAGCAGTGGTATCAACGCAGA13450.22107965771280355No Hit
CCCATATTCAGACAGGATACCACGT12730.20924491023672784No Hit
GTATTTAGCCTTGGAGGATGGTCCC12070.19839639171699172No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11920.19593081932614262No Hit
GTACTCTGCGTTGATACCACTGCTT11760.19330087544257024No Hit
TATTCAGACAGGATACCACGTGTCC11520.18935595961721166No Hit
GTTCACTATCGGTCAGTCAGGAGTA11460.18836973066087204No Hit
GGTTAATGAGGCGAACCGGGGGAAC11360.18672601573363928No Hit
GATACCACTGCTTATGTACTCTGCG11220.18442481483551346No Hit
CCACTGCTTATGTACTCTGCGTTGA11060.18179487095194108No Hit
TCTAAGTACCCCGAGGAAAAGAAAT10880.17883618408292215No Hit
GCCTTGGAGGATGGTCCCCCCATAT10570.17374066780850062No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA10170.16716580809956968No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG10150.1668370651141231No Hit
GTTGATACCACTGCTTATGTACTCT10110.16617957914323003No Hit
GCAGTGGTATCAACGCAGAGTACAT9880.16239903481059473No Hit
GCCCAGAGCCTGAATCAGTGTGTGT9660.1587828619706827No Hit
ATACCACTGCTTATGTACTCTGCGT9430.1550023176380474No Hit
GAGTACATGGGAAGCAGTGGTATCA9370.15401608868170774No Hit
CTATCGGTCAGTCAGGAGTATTTAG9200.15122177330541212No Hit
GTTAATGATAGTGTGTCGAAACACA9070.14908494390000954No Hit
GTACATGATAAGCAGTGGTATCAAC8890.14612625703099058No Hit
CATCTAAGTACCCCGAGGAAAAGAA8620.1416882267274622No Hit
GTCAGGAGTATTTAGCCTTGGAGGA8240.1354421100039778No Hit
ATCATGTACTCTGCGTTGATACCAC7780.12788102133870718No Hit
CACACACACTGATTCAGGCTCTGGG7660.12590856342602788No Hit
CCCCAGTAGCGGCGAGCGAACGGGG7640.12557982044058136No Hit
CAGTGGTATCAACGCAGAGTACATA7300.11999118968799004No Hit
AAACAAAAAAAAAAAAAAAAAAAAA7290.11982681819526675No Hit
GGTATCAACGCAGAGTACATGGGAA7280.11966244670254349No Hit
TACCACTGCTTATGTACTCTGCGTT7250.11916933222437366No Hit
GAACAAAAAAAAAAAAAAAAAAAAA7240.11900496073165039No Hit
GCCTGAATCAGTGTGTGTGTTAGTG7060.11604627386263146No Hit
GAATCAGTGTGTGTGTTAGTGGAAG6960.11440255893539872No Hit
GCGTTGATACCACTGCTTCCCATGT6890.1132519584863358No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN6880.11308758699361253No Hit
TTCCAGACGCTTCCACTAACACACA6790.11160824355910305No Hit
GCAGAGTACATAAGCAGTGGTATCA6780.11144387206637979No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG6730.11062201460276341No Hit
GAGTACATGTAAGCAGTGGTATCAA6540.10749895624102121No Hit
TGATACCACTGCTTATGTACTCTGC6520.10717021325557466No Hit
GTATCAACGCAGAGTACATGGGAAG6500.10684147027012811No Hit
GCCCTACTCATCGAGCTCACAGCAT6380.10486901235744882No Hit
GGAGTATTTAGCCTTGGAGGATGGT6360.10454026937200228No Hit
GTCCCGCCCTACTCATCGAGCTCAC6280.10322529743021609No Hit
GGTTCACTATCGGTCAGTCAGGAGT6240.10256781145932298No Hit
TCACGGTACTGGTTCACTATCGGTC6170.10141721101026006No Hit
ACGCAGAGTACATGGGAAGCAGTGG6150.10108846802481353No Hit
CATGTAAGCAGTGGTATCAACGCAG6120.1005953535466437No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACTC250.00603760718.99472414
ACAGGTA250.00603760718.99472411
TACAAGA504.5222714E-617.1121792
GCAAATT508.720618E-515.1957813
GACTGTA456.7639275E-414.7748937
GTACAAA1203.6379788E-1214.2625041
GTCTAGG400.005243586414.2625041
GATTAAG400.00524933214.260152
AGACCTG400.0052868114.24486919
GCGTGCT400.0052868114.24486918
GTACAAG551.9425416E-413.8303061
GGCTGAA500.001501779713.29521218
CGCTACT801.9777945E-613.0718042
CTGGCAA604.0895253E-412.66523710
TGTCCCG4650.012.45890511
CCGCTAC853.893745E-612.3049051
GTGTCCC4600.012.18340810
TACTACA550.00304843412.0995212
ACAAGAT550.003050318712.0985223
CTGTAAC550.003067328112.0895459
Produced by FastQC (version 0.11.5)