FastQCFastQC Report
Wed 25 May 2016
SRR1295186_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295186_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608378
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA198833.2681983898168574No Hit
GTATCAACGCAGAGTACTTTTTTTT126312.0761763245876743No Hit
GCTTATGTACTCTGCGTTGATACCA115071.8914227667667143No Hit
GGTATCAACGCAGAGTACTTTTTTT91931.511067132605058No Hit
CTTATGTACTCTGCGTTGATACCAC84481.388610370526219No Hit
TATCAACGCAGAGTACTTTTTTTTT80981.3310803480730728No Hit
GTACTTTTTTTTTTTTTTTTTTTTT56360.9263977329883724No Hit
GAGTACATAAGCAGTGGTATCAACG53100.872812626360585No Hit
ACGCAGAGTACTTTTTTTTTTTTTT47630.7829014198409541No Hit
CATAAGCAGTGGTATCAACGCAGAG39320.6463087093879135No Hit
CCCATGTACTCTGCGTTGATACCAC39280.6456512234170203No Hit
GCGTTGATACCACTGCTTATGTACT38040.6252691583193344No Hit
TATGTACTCTGCGTTGATACCACTG37210.6116263244233027No Hit
CTGCTTATGTACTCTGCGTTGATAC30740.5052779686313443No Hit
ACGCAGAGTACATAAGCAGTGGTAT30540.5019905387768788No Hit
GAGTACTTTTTTTTTTTTTTTTTTT28580.46977372620311714No Hit
CATGTACTCTGCGTTGATACCACTG26870.44166620094743725No Hit
ATACAGGGTGACAGCCCCGTACACA26700.43887188557114165No Hit
GTACTGGTTCACTATCGGTCAGTCA26040.42802336705140553No Hit
GTATCAACGCAGAGTACATAAGCAG24620.40468261508470066No Hit
ACTCTGCGTTGATACCACTGCTTAT24610.4045182435919774No Hit
GTACATGGGAAGCAGTGGTATCAAC23470.38577989342152413No Hit
GGATACCACGTGTCCCGCCCTACTC22970.3775613187853604No Hit
GTGGTATCAACGCAGAGTACATGGG20810.3420570763571332No Hit
TATCAACGCAGAGTACATAAGCAGT20800.34189270486441No Hit
GGTATCAACGCAGAGTACATAAGCA20630.33909838948811427No Hit
GCAGAGTACTTTTTTTTTTTTTTTT19280.3169082379704723No Hit
ACCCTGTATCGCGCGCCTTTCCAGA18850.30984026378337154No Hit
AAGCAGTGGTATCAACGCAGAGTAC16770.27565099329693055No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA16350.26874739060255304No Hit
ACATAAGCAGTGGTATCAACGCAGA15270.2509952693884394No Hit
GTGGTATCAACGCAGAGTACATAAG15160.24918718296848338No Hit
CCCATATTCAGACAGGATACCACGT14980.24622849609946448No Hit
ATCATTAACTGAATCCATAGGTTAA13450.22107965771280355No Hit
ATCAACGCAGAGTACTTTTTTTTTT13170.21647725591655187No Hit
GTGGTATCAACGCAGAGTACTTTTT12610.20727245232404853No Hit
GATACCACTGCTTATGTACTCTGCG11930.19609519081886592No Hit
CTATCGGTCAGTCAGGAGTATTTAG11880.19527333335524955No Hit
CCACTGCTTATGTACTCTGCGTTGA11750.193136503949847No Hit
GGTTAATGAGGCGAACCGGGGGAAC11320.18606852976274618No Hit
GTTCACTATCGGTCAGTCAGGAGTA11140.18310984289372725No Hit
GCCCAGAGCCTGAATCAGTGTGTGT11100.18245235692283415No Hit
GTACTCTGCGTTGATACCACTGCTT11080.1821236139373876No Hit
GTATTTAGCCTTGGAGGATGGTCCC10830.17801432661930577No Hit
GAGTACATGGGAAGCAGTGGTATCA10270.1688095230268024No Hit
GCCTTGGAGGATGGTCCCCCCATAT10210.16782329407046276No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG10200.1676589225777395No Hit
GCAGTGGTATCAACGCAGAGTACAT10160.1670014366068464No Hit
TCTAAGTACCCCGAGGAAAAGAAAT9950.16354963525965766No Hit
TATTCAGACAGGATACCACGTGTCC9890.162563406303318No Hit
GTCAGGAGTATTTAGCCTTGGAGGA9750.16026220540519218No Hit
ATACCACTGCTTATGTACTCTGCGT9700.1594403479415758No Hit
CATCTAAGTACCCCGAGGAAAAGAA9400.15450920315987757No Hit
CACACACACTGATTCAGGCTCTGGG9190.1510574018126888No Hit
GTTAATGATAGTGTGTCGAAACACA9110.14974242987090264No Hit
ATCATGTACTCTGCGTTGATACCAC9020.14826308643639316No Hit
GTTGATACCACTGCTTATGTACTCT8960.14727685748005354No Hit
GTACATGATAAGCAGTGGTATCAAC8890.14612625703099058No Hit
GCCTGAATCAGTGTGTGTGTTAGTG8690.14283882717652513No Hit
CCCCAGTAGCGGCGAGCGAACGGGG8270.13593522448214762No Hit
GGTATCAACGCAGAGTACATGGGAA8120.13346965209129852No Hit
GAATCAGTGTGTGTGTTAGTGGAAG7770.1277166498459839No Hit
GCAGAGTACATAAGCAGTGGTATCA7720.12689479238236753No Hit
CAGTGGTATCAACGCAGAGTACATA7630.12541544894785805No Hit
TACCACTGCTTATGTACTCTGCGTT7580.12459359148424169No Hit
GCGTTGATACCACTGCTTCCCATGT7520.12360736252790207No Hit
CATGTAAGCAGTGGTATCAACGCAG7180.11801873177531075No Hit
ACGCAGAGTACATGGGAAGCAGTGG7140.11736124580441765No Hit
GTCCCGCCCTACTCATCGAGCTCAC7110.11686813132624782No Hit
AGCTCACAGCATGTGCATTTTTGTG7080.116375016848078No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG7050.1158819023699082No Hit
GTATCAACGCAGAGTACATGGGAAG7020.11538878789173837No Hit
GCTTACATGTACTCTGCGTTGATAC7000.1150600449062918No Hit
GATTCAGGCTCTGGGCTGCTCCCCG6910.11358070147178236No Hit
CGGTACTGGTTCACTATCGGTCAGT6830.11226572952999615No Hit
TCACGGTACTGGTTCACTATCGGTC6740.11078638609548667No Hit
CCCCATGTACTCTGCGTTGATACCA6720.11045764311004014No Hit
TGATACCACTGCTTATGTACTCTGC6720.11045764311004014No Hit
GGTATCAACGCAGAGTACATGGGGG6650.10930704266097722No Hit
GAGTACATGTAAGCAGTGGTATCAA6510.10700584176285138No Hit
GGTTCACTATCGGTCAGTCAGGAGT6420.1055264983283419No Hit
GCCCTACTCATCGAGCTCACAGCAT6420.1055264983283419No Hit
CAGTAGCGGCGAGCGAACGGGGAGC6410.10536212683561864No Hit
GGAGTATTTAGCCTTGGAGGATGGT6180.10158158250298335No Hit
TTCCAGACGCTTCCACTAACACACA6130.10075972503936698No Hit
TATCAACGCAGAGTACATGGGAAGC6120.1005953535466437No Hit
GTATCGCGCGCCTTTCCAGACGCTT6120.1005953535466437No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGCCG250.00602087319.00386410
CGATCTT250.00602087319.00386410
TTATACA451.6670019E-618.9976162
GAGCCGA504.5442866E-617.10347711
CTTATAC402.7568333E-416.6256471
TCCAGGG402.7602937E-416.6229133
GGGCGAT350.00216521416.2890267
TGGCGTC350.002168784616.285015
CGAGTGG350.00217116816.28233316
GTACAAG601.4626366E-615.833951
TACAAGA601.4651469E-615.83134652
TATACAC551.1273974E-515.5435043
CCCCTGG456.751969E-414.7783531
TGAAGTG456.756179E-414.7771385
AAAAGTT456.756179E-414.7771385
ATATAAT400.00527573214.2493836
AGGGCGA400.00527573214.2493836
GAATAAA551.9564203E-413.818721
ACAAGAT551.9591514E-413.8164483
ACAGGGT4050.013.6032313
Produced by FastQC (version 0.11.5)