FastQCFastQC Report
Wed 25 May 2016
SRR1295185_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295185_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5722028
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCATTCCATTCCATTCCATTCC71210.12444888420678822No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN62190.1086852423651195No Hit
GTATCAACGCAGAGTACTTTTTTTT60550.10581912566663426No Hit
GAATGGAATGGAATGGAATGGAATG59260.103564680214777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTCG4450.010.89349652
TCGGGCG1553.6779966E-910.4180996
GGTATCA34900.010.4046041
CTGGTCG1901.0004442E-109.9985319
ACACCGT2251.8189894E-129.709946
GTGGTAT17600.09.6678081
ACTATCG800.0045233929.4987728
CCGCGCG900.00111597149.4986059
TACACCG1451.6964368E-69.1772665
GTCGTAC1151.10433604E-49.0924831
TGGTATC17150.09.0894342
GTCTAGC2850.09.0054731
CACGGTA950.00182686928.9989947
AGCCGCG1804.1898602E-88.9770472
TAAACGC850.00740718.9459854
AGGTCGC850.00744719668.9400996
CTCGGGC4050.08.9182965
GCACCGT2356.548362E-118.8925466
GTATTAG8850.08.8076121
AAGACCG1951.6372724E-88.773875
Produced by FastQC (version 0.11.5)