FastQCFastQC Report
Wed 25 May 2016
SRR1295185_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295185_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5722028
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT148710.25989037453154723No Hit
GGTATCAACGCAGAGTACTTTTTTT114670.20040097671664658No Hit
TATCAACGCAGAGTACTTTTTTTTT84350.14741277043733444No Hit
ATTCCATTCCATTCCATTCCATTCC75270.13154427066767235No Hit
GAATGGAATGGAATGGAATGGAATG66670.11651463432195719No Hit
GTGGTATCAACGCAGAGTACATGGG62630.10945420050373748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44750.011.6789321
GTGGTAT18250.011.1946131
ACGTCTA1002.4012399E-510.4500885
TGGTATC17750.010.3829132
CTAGTAC3450.010.1883133
ACCGATC1401.0596286E-69.5003278
TAAACCG1106.8426845E-59.5000795
AGCGTCA4000.09.4999123
AAGACCG1554.266567E-79.19362455
GTCCTAG6900.08.951541
ACCGTCC850.0074377378.9414858
ACCGACC1107.147252E-48.6366628
CTAGGAC6250.08.5119223
TAGGACT7850.08.4712594
CTAGACT5300.08.4244514
TAACCCG3050.08.4099065
CTAATAC6550.08.2670993
ATCGCTA1150.0011110068.26115510
CTAGAAC7600.08.2499253
GTATCAA79150.08.1877951
Produced by FastQC (version 0.11.5)