FastQCFastQC Report
Wed 25 May 2016
SRR1295184_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295184_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6718052
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG95690.14243712314224422No Hit
GTATCAACGCAGAGTACTTTTTTTT86200.12831100443997753No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN78030.11614974102611889No Hit
GGTATCAACGCAGAGTACATGGGGG70850.10546211907856622No Hit
GGTATCAACGCAGAGTACTTTTTTT68340.10172591697712371No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACT701.09402325E-412.21179317
GTGGTAT23050.011.257491
TGGTATC23150.010.6743882
GGTATCA49800.010.573781
CGTAGCG1856.002665E-1110.2749313
GTCTTAG6500.09.9436411
TATTCCG3150.09.6549565
GCGCGAC1002.7629326E-49.49827416
GTGTAAG8500.08.9458351
GTACCGT2151.0459189E-98.8364626
TACACTG12550.08.7846635
GTATCAA89000.08.6505731
ACACCGT2106.4028427E-98.5945116
GTATTAG10300.08.582141
TGTACCG2553.8198777E-118.5723245
CCGTGCG1356.813121E-58.4436049
TAGACAG8800.08.3160855
GTCCTAG7750.08.2171831
TTTAGCG1401.0346837E-48.1465523
AATACCG1752.4282836E-68.1463695
Produced by FastQC (version 0.11.5)