FastQCFastQC Report
Wed 25 May 2016
SRR1295184_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295184_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6718052
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT222810.33165864152287No Hit
GGTATCAACGCAGAGTACTTTTTTT180650.2689023544325051No Hit
TATCAACGCAGAGTACTTTTTTTTT129900.19335962270015178No Hit
GTGGTATCAACGCAGAGTACATGGG100330.1493438871863451No Hit
ACGCAGAGTACTTTTTTTTTTTTTT83180.1238156537043774No Hit
GGTATCAACGCAGAGTACATGGGGG72870.10846894308052393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63800.012.1240331
AGCGTCA5200.011.6939663
GTGGTAT24500.011.3643581
TGGTATC26200.010.262832
CGGATAG1554.062349E-89.8079165
CGACGTA1002.758919E-49.499659
GTATTAG9300.09.2982641
GTTCTAG6950.09.16081
GCGACGT950.0018257128.9996688
TCGGTAC850.0074314728.9424453
TACGTCG1606.737173E-78.9075795
GTATCAA117900.08.817531
GTCCTAA5350.08.7033361
TATACTG10900.08.6297285
GTCTTAG5850.08.6092121
TAAGTCG1000.00290307388.5512775
GTGCTAG5750.08.42841151
GTCCTAG6800.08.38466551
ATAGTAC6800.08.3835423
GCCTACG1606.4343785E-68.3147941
Produced by FastQC (version 0.11.5)