FastQCFastQC Report
Wed 25 May 2016
SRR1295183_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295183_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553703
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA265264.790654917889194No Hit
GTACATAAGCAGTGGTATCAACGCA105351.902644558544924No Hit
GCTTATGTACTCTGCGTTGATACCA62591.1303893964815073No Hit
GTATCAACGCAGAGTACTTTTTTTT48490.875740243415694No Hit
CTTATGTACTCTGCGTTGATACCAC43370.783271898472647No Hit
GGTATCAACGCAGAGTACTTTTTTT33340.6021278555471075No Hit
GAGTACATAAGCAGTGGTATCAACG30150.5445157421939198No Hit
TATCAACGCAGAGTACTTTTTTTTT30100.5436127310128355No Hit
TATGTACTCTGCGTTGATACCACTG25430.45927148669954837No Hit
GCGTTGATACCACTGCTTATGTACT21940.39624130625985415No Hit
CATAAGCAGTGGTATCAACGCAGAG19160.346033884591559No Hit
GTGGTATCAACGCAGAGTACATGGG18110.3270706497887857No Hit
GTACTGGTTCACTATCGGTCAGTCA17200.3106358462930488No Hit
CTGCTTATGTACTCTGCGTTGATAC17180.31027464182061504No Hit
ACGCAGAGTACATAAGCAGTGGTAT16280.29402044056109505No Hit
ATACAGGGTGACAGCCCCGTACACA14480.26151203804205503No Hit
ACTCTGCGTTGATACCACTGCTTAT14350.2591642089712355No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14140.25537156201068084No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13980.2524819262312106No Hit
GGATACCACGTGTCCCGCCCTACTC13430.2425488032392817No Hit
GTATCAACGCAGAGTACATAAGCAG13060.23586652049925683No Hit
TATCAACGCAGAGTACATAAGCAGT12210.22051533042082125No Hit
GGTATCAACGCAGAGTACATAAGCA11830.2136524454445795No Hit
CCCATGTACTCTGCGTTGATACCAC10480.1892711435552995No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA10380.18746512119313063No Hit
ACCCTGTATCGCGCGCCTTTCCAGA10310.18620090553961238No Hit
CATGTACTCTGCGTTGATACCACTG10040.1813246451617564No Hit
ACATAAGCAGTGGTATCAACGCAGA9250.16705706850062219No Hit
GTGGTATCAACGCAGAGTACATAAG8930.16127779694168173No Hit
AAGCAGTGGTATCAACGCAGAGTAC8410.15188648065840352No Hit
CCCATATTCAGACAGGATACCACGT7670.13852191517835374No Hit
GAGTACTTTTTTTTTTTTTTTTTTT7530.1359934838713173No Hit
CCACTGCTTATGTACTCTGCGTTGA7220.13039481454859375No Hit
GTATTTAGCCTTGGAGGATGGTCCC7160.1293112011312924No Hit
AAACAAAAAAAAAAAAAAAAAAAAA7010.12660216758803908No Hit
GCCTTGGAGGATGGTCCCCCCATAT6790.12262891839126752No Hit
GTACATGATAAGCAGTGGTATCAAC6780.12244831615505063No Hit
ATCATGTACTCTGCGTTGATACCAC6750.12190650944639997No Hit
GATACCACTGCTTATGTACTCTGCG6560.11847506695827907No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN6380.11522422670637508No Hit
TCTAAGTACCCCGAGGAAAAGAAAT6340.11450181776150753No Hit
GTTCACTATCGGTCAGTCAGGAGTA6180.11161218198203729No Hit
GCCCAGAGCCTGAATCAGTGTGTGT5880.10619411489553063No Hit
GCAGAGTACTTTTTTTTTTTTTTTT5810.1049298992420124No Hit
ATACCACTGCTTATGTACTCTGCGT5630.10167905899010841No Hit
GTTGATACCACTGCTTATGTACTCT5630.10167905899010841No Hit
CGGTACTGGTTCACTATCGGTCAGT5570.10059544557280709No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACCT250.00601365219.0074355
TAATCAT307.6938345E-419.0074335
GCACATC250.006035730718.9953817
CAGGACC350.002160847216.2935644
TTGTCCT456.727927E-414.7848994
TATGACT400.0052565614.2568684
TCATGGT400.0052565614.2568684
CATGTTA400.00527556414.24911810
CGAACCG1350.014.07320212
CGGGGGA1350.014.07065217
AACCGGG1400.013.57058814
GCGAACC1400.013.57058811
TAAGACA500.001491982313.306414
GTCTAGA655.4201315E-513.1589932
GGTCTAG604.064914E-412.6739211
ACCGGGG1501.8189894E-1212.66588215
GGCGAAC1551.8189894E-1212.25730510
CCGGGGG1551.8189894E-1212.25508416
CACACCC550.00305516912.095645
CGTCTCC550.003065558412.09015912
Produced by FastQC (version 0.11.5)