##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295183_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	553703
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.52795451713283	31.0	28.0	33.0	16.0	34.0
2	28.466087415094375	31.0	28.0	33.0	16.0	34.0
3	28.75573547551666	31.0	28.0	34.0	16.0	34.0
4	31.539863428588973	35.0	32.0	37.0	17.0	37.0
5	32.363295846329166	35.0	32.0	37.0	19.0	37.0
6	32.17544784839526	35.0	32.0	37.0	17.0	37.0
7	32.23994090694831	35.0	32.0	37.0	17.0	37.0
8	32.22382035134359	35.0	32.0	37.0	17.0	37.0
9	32.88712540838681	37.0	31.0	39.0	17.0	39.0
10	33.051975517560855	37.0	32.0	39.0	17.0	39.0
11	33.43833426945493	37.0	32.0	39.0	17.0	39.0
12	33.53244248270282	37.0	32.0	39.0	17.0	39.0
13	33.32214743282951	37.0	32.0	39.0	17.0	39.0
14	33.760579227491995	38.0	31.0	40.0	17.0	41.0
15	34.067783631296926	38.0	32.0	41.0	17.0	41.0
16	33.988278914869525	38.0	32.0	40.0	17.0	41.0
17	33.73162146493698	38.0	32.0	40.0	17.0	41.0
18	33.610533083620645	38.0	31.0	40.0	17.0	41.0
19	33.95812917755548	38.0	32.0	40.0	18.0	41.0
20	33.97829341722909	38.0	32.0	40.0	18.0	41.0
21	34.032902115393995	38.0	32.0	40.0	18.0	41.0
22	33.9410947746355	38.0	31.0	40.0	18.0	41.0
23	33.63040294164922	38.0	31.0	40.0	17.0	41.0
24	33.93888781530893	38.0	31.0	40.0	18.0	41.0
25	32.68004146627344	37.0	29.0	40.0	16.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.19041127113414902
1101	2	-0.07396077390346889
1101	3	-0.10495628486596331
1101	4	-0.1470459585260251
1101	5	-0.19085010088520704
1101	6	-0.48504071070912147
1101	7	-0.5939242807719936
1101	8	-0.5288800195900549
1101	9	-0.07879060652368963
1101	10	-0.10226629422119515
1101	11	-0.3628107806764902
1101	12	-0.4470781226512628
1101	13	-0.40940808148992147
1101	14	0.04585253679973533
1101	15	0.22218534252065325
1101	16	0.22310914045635855
1101	17	-0.09476517965285325
1101	18	-0.011926192624216014
1101	19	-0.2702000535078497
1101	20	-0.3371924296247002
1101	21	-0.6479642315267711
1101	22	-0.6292093599144266
1101	23	-0.39117350911749327
1101	24	-0.44811909403078687
1101	25	-0.3003501990221764
1102	1	-0.4569183645179393
1102	2	-0.3082789709139142
1102	3	-0.49063685377642585
1102	4	-0.31614564640685217
1102	5	-0.5684448807306417
1102	6	-0.6640361703264546
1102	7	-0.45131615744635667
1102	8	-0.35483116034191653
1102	9	-0.21222540858254746
1102	10	0.37046055779729414
1102	11	-0.005578305885123314
1102	12	-0.32179081361214656
1102	13	-0.3064318940744428
1102	14	-0.018599814892233724
1102	15	-0.16906400830232116
1102	16	-0.2922577152643697
1102	17	-0.2799695997128069
1102	18	-0.31933786598404623
1102	19	-0.42196350111348124
1102	20	-0.3251935814318543
1102	21	-0.4483930740119959
1102	22	-0.29807508696698193
1102	23	-0.5413969288535085
1102	24	-0.6996835463824809
1102	25	-0.22206330648475614
1103	1	-0.2919914065743292
1103	2	-0.06493142853100764
1103	3	-0.13768766184113446
1103	4	-0.017249118796904384
1103	5	-0.047509243097401566
1103	6	-0.0832348416346278
1103	7	-0.2970845516523539
1103	8	-0.11353013245687293
1103	9	0.5656789194356762
1103	10	0.5106005229345598
1103	11	0.1010718378336648
1103	12	-0.039628912718981724
1103	13	0.519485823701956
1103	14	0.25804215305255696
1103	15	0.3090927917305848
1103	16	0.4488427747678756
1103	17	0.6264537819724296
1103	18	0.6720467193396686
1103	19	0.24447263272239894
1103	20	0.17183691574776105
1103	21	0.20305156982763606
1103	22	0.1913324008079229
1103	23	-2.13948050686016
1103	24	-0.6964260917734535
1103	25	0.1578890786301983
1104	1	-0.6799202292088538
1104	2	-0.28391637875816755
1104	3	-0.6054461087107939
1104	4	-0.5780222629735725
1104	5	-0.7169584893129262
1104	6	-0.2784348271213304
1104	7	-0.7308144954392368
1104	8	-0.23622452643592595
1104	9	-2.5462613790717867
1104	10	-0.8886020231127674
1104	11	-0.4383343609000434
1104	12	-0.302555467650528
1104	13	-0.22697461589550727
1104	14	0.03095048861040084
1104	15	-0.11575293967489841
1104	16	-0.5026918770295765
1104	17	0.0798699793325639
1104	18	0.5237208024749549
1104	19	-0.12578624825040663
1104	20	-0.06580539881245073
1104	21	-0.13241426819876523
1104	22	-0.030897308433679882
1104	23	-0.2741302309778959
1104	24	-0.24176200985624519
1104	25	-0.43338143112352157
1105	1	-0.0931149151199584
1105	2	-0.21968150915016338
1105	3	-0.30222813265699955
1105	4	-0.09006949931931629
1105	5	-0.04110872713480518
1105	6	-0.42443187484972356
1105	7	-0.2680229586178484
1105	8	-0.5871708935016962
1105	9	-1.7457496519928952
1105	10	-0.3764668746791173
1105	11	-0.456646317600061
1105	12	-0.3220745754084362
1105	13	-0.1771875074815341
1105	14	-0.10407329357755657
1105	15	-0.34419840465767493
1105	16	-0.11280701562232309
1105	17	-1.3674372209298582
1105	18	-0.09992361281125284
1105	19	-0.10344739307573292
1105	20	0.006362552639082253
1105	21	-0.22347019734424833
1105	22	-0.27519581589636744
1105	23	-0.1924389073761219
1105	24	-0.21992044843259606
1105	25	-0.6537652264326859
1106	1	-0.529982076978655
1106	2	-0.603457636287839
1106	3	-0.6156915978496045
1106	4	-0.28122099353247876
1106	5	-0.8028762172021757
1106	6	-0.7267763212135172
1106	7	-0.4954681132214098
1106	8	-0.49637110079138225
1106	9	-2.6755544542039864
1106	10	-1.2069826805177755
1106	11	-0.5915960498508852
1106	12	-0.6207278834063672
1106	13	-0.42189712917785727
1106	14	-0.6128174635551034
1106	15	-0.6141959862779913
1106	16	-0.571182555439222
1106	17	-0.6778908398623145
1106	18	-0.2506715830997308
1106	19	-0.4147965034574952
1106	20	-0.6276149909781168
1106	21	-0.32901243261804325
1106	22	-0.2251256828854835
1106	23	-0.020537715460420713
1106	24	-0.443968816026306
1106	25	-1.1501963889823514
1107	1	-0.03307442018182627
1107	2	0.13500915451687945
1107	3	0.13799683922151829
1107	4	-1.0416735488131295
1107	5	-0.11835507134178158
1107	6	-0.017313764613504645
1107	7	0.1316620807522284
1107	8	0.28868962393362096
1107	9	0.0724280757911302
1107	10	0.35215404770558933
1107	11	0.14760115146630426
1107	12	-0.5296156327762702
1107	13	-0.15944172570425508
1107	14	-0.10645799548981927
1107	15	-0.7507405868848949
1107	16	-0.7719026286706949
1107	17	-0.38387816581175116
1107	18	-0.07706646995411148
1107	19	0.13007341682303064
1107	20	-0.009943409115692248
1107	21	0.048088857773862514
1107	22	0.09404602887303781
1107	23	0.5753181229950783
1107	24	0.5411414188339947
1107	25	-0.1114030217082771
1108	1	-0.020011852785120965
1108	2	0.046297038961135684
1108	3	-0.05398936380887065
1108	4	0.5291638288323988
1108	5	0.34770147028474696
1108	6	0.18405809872403722
1108	7	0.23252732896181527
1108	8	0.0010585484383014432
1108	9	0.2430456223736499
1108	10	0.45352970545457083
1108	11	0.5069588432891052
1108	12	0.528836663515861
1108	13	0.3244558937309634
1108	14	0.2923762633943028
1108	15	0.3209419653096788
1108	16	0.10317433056141567
1108	17	0.6514416847129212
1108	18	0.28295218386215026
1108	19	0.5287926193601464
1108	20	0.18747369829429772
1108	21	0.28968882643936666
1108	22	0.379185032852277
1108	23	0.3153789070184061
1108	24	0.1971241652779625
1108	25	0.514355401579131
1109	1	0.5386602605240682
1109	2	0.4027072496320372
1109	3	0.5185681202704018
1109	4	0.6225737481436049
1109	5	0.5825540363312385
1109	6	0.7519240970472296
1109	7	0.7548341842392929
1109	8	0.8054598637192214
1109	9	1.0853510505832205
1109	10	0.8975190737986409
1109	11	0.7602966956708173
1109	12	0.911792715770325
1109	13	0.7601302130493011
1109	14	0.9330227251578052
1109	15	0.9118015953423253
1109	16	0.8805231951505093
1109	17	1.2740428727469535
1109	18	0.8662590569779738
1109	19	0.7398000698438594
1109	20	0.6594585713744507
1109	21	0.7753307394239002
1109	22	0.6626839655244012
1109	23	1.034371397073528
1109	24	0.7486478575783337
1109	25	0.9991933996406956
1110	1	0.5963795903745464
1110	2	0.7449338754652217
1110	3	0.6828268123979448
1110	4	0.8556008303510119
1110	5	0.6823198442937652
1110	6	0.7077879053700542
1110	7	0.4735155029206126
1110	8	0.5684335021026996
1110	9	1.0535910073477623
1110	10	0.5272254330131929
1110	11	0.276804231850484
1110	12	0.43423311689925725
1110	13	0.4375817232876926
1110	14	0.6005420910378234
1110	15	0.4942191777599092
1110	16	0.3368193580040497
1110	17	0.913837504344869
1110	18	0.9866697937821556
1110	19	0.7361130464847037
1110	20	0.8852196954962253
1110	21	0.8194309015568493
1110	22	0.48265033794978507
1110	23	0.8216709827337638
1110	24	0.5173982328860873
1110	25	0.625795213127752
1111	1	0.3252411091689531
1111	2	0.293747701460255
1111	3	0.24809465417585486
1111	4	0.6297655917640945
1111	5	0.7661165844663458
1111	6	0.6035029286018627
1111	7	0.6261988374831375
1111	8	0.3661816152870543
1111	9	1.0824574429256089
1111	10	0.7321250077114243
1111	11	0.5425022921794209
1111	12	0.5137694642208359
1111	13	0.3384519939566246
1111	14	0.34852498063145276
1111	15	0.45766289288147277
1111	16	0.5547557839941675
1111	17	0.8656481098467452
1111	18	0.8257778892660852
1111	19	0.6260508491741845
1111	20	-0.15881993377829673
1111	21	0.060958054493404745
1111	22	0.2692963541260056
1111	23	0.36038819393419885
1111	24	0.6255650101803809
1111	25	0.6473725172553912
1112	1	0.11939392543707328
1112	2	0.1954460139291676
1112	3	0.35273040278346457
1112	4	0.6854673493741608
1112	5	0.5966994406010713
1112	6	0.4846014532679419
1112	7	0.3035746004495863
1112	8	0.19989842272424596
1112	9	-1.3779136325906123
1112	10	-0.022510373349120982
1112	11	0.0969984954182408
1112	12	0.33454003889292494
1112	13	0.1152149334510284
1112	14	0.5522746957824793
1112	15	0.26558034671143815
1112	16	0.14305529074600543
1112	17	0.4690979065932197
1112	18	0.4270799859995762
1112	19	0.3427112675407571
1112	20	0.1428945507614472
1112	21	0.11887122027427921
1112	22	0.37382061345741135
1112	23	0.03697495586906285
1112	24	-0.1325951472277751
1112	25	-0.08045275577082123
1113	1	0.1286761484420289
1113	2	0.38199879292661265
1113	3	0.3468341706784308
1113	4	0.5713364163509134
1113	5	0.12297434397910223
1113	6	0.3772219955089966
1113	7	0.14470202564898216
1113	8	0.5845660479709309
1113	9	0.0877053511537369
1113	10	0.2621070711987983
1113	11	0.43808370127973717
1113	12	0.18755160457897802
1113	13	0.10228134085075169
1113	14	-0.05065605002815943
1113	15	0.23448882001192572
1113	16	0.4112735381850996
1113	17	0.4792172787554776
1113	18	0.3382890995864827
1113	19	0.41171937847870055
1113	20	0.4312864041180049
1113	21	0.6407311871743175
1113	22	0.7975266510639898
1113	23	0.5341727980990214
1113	24	0.23652008020176396
1113	25	0.19907303662584042
1114	1	0.2998839600206473
1114	2	0.19687642110855208
1114	3	0.38209132741049245
1114	4	0.7344794320642585
1114	5	0.6341758622915137
1114	6	0.5226187438229459
1114	7	0.5838555565677552
1114	8	0.368554640806515
1114	9	0.09829984182037776
1114	10	0.2272946551296613
1114	11	0.3005820288566312
1114	12	0.5120041647490439
1114	13	0.21851103020125606
1114	14	0.8384238942739586
1114	15	0.45623354134907146
1114	16	0.534059126109959
1114	17	0.5812511930183959
1114	18	0.47748921058582283
1114	19	0.2875537317836603
1114	20	0.6435998979709225
1114	21	0.3877963943317937
1114	22	0.3392653764545912
1114	23	0.646184264727367
1114	24	0.6288804514549184
1114	25	0.5488894417337917
1115	1	0.6128119951790438
1115	2	0.5651357665973435
1115	3	0.48162568794100835
1115	4	0.8523424608135777
1115	5	0.7937816383136962
1115	6	0.850990052060574
1115	7	1.1106481377276651
1115	8	0.9291413988571051
1115	9	1.318004500831023
1115	10	0.8502640223308546
1115	11	0.7024367388783403
1115	12	0.9474071521663134
1115	13	0.7755832566380647
1115	14	0.7479865070868925
1115	15	0.7885457813888337
1115	16	0.7671796953411274
1115	17	1.2116524800668174
1115	18	1.4259235632684835
1115	19	1.0631639026053321
1115	20	0.9290735160942347
1115	21	0.9758454172736961
1115	22	0.1532227887547961
1115	23	0.7365444813281954
1115	24	0.7965446678464758
1115	25	0.5076633449958834
1116	1	0.838834820329776
1116	2	1.040609496140842
1116	3	1.0683935604646955
1116	4	1.457941082691267
1116	5	1.4434640241879464
1116	6	1.4884146859968368
1116	7	1.2201119620170715
1116	8	1.0255076779268748
1116	9	1.3700770675838214
1116	10	1.0159616967494571
1116	11	1.1450454813417394
1116	12	1.0387508214169614
1116	13	1.225297942253917
1116	14	1.554683320179052
1116	15	1.4476812249719515
1116	16	1.5994363706210635
1116	17	1.623781033038398
1116	18	1.8017364400988
1116	19	1.4286623952840571
1116	20	1.7973228700994
1116	21	1.5845178978938463
1116	22	1.688526406325856
1116	23	2.041448658761446
1116	24	1.58121844045629
1116	25	1.5301170486995872
1201	1	0.016222318749697706
1201	2	0.04760924332830285
1201	3	-0.17469359665829742
1201	4	-0.08664872896339304
1201	5	-0.26674565221200197
1201	6	-0.4177918618479346
1201	7	-0.3157005142817546
1201	8	-0.39681171911521673
1201	9	0.24429097859380988
1201	10	0.26752597433762304
1201	11	0.21576583907148006
1201	12	0.2331004611590899
1201	13	-0.011812568427416181
1201	14	0.02606810086417255
1201	15	-0.3066284387873708
1201	16	0.04599812008383708
1201	17	-0.1713104534993377
1201	18	0.07846741595520257
1201	19	-0.06538465944986172
1201	20	-0.09277477874397988
1201	21	-0.3648919428342481
1201	22	-0.13046771578260064
1201	23	-0.3890792454226073
1201	24	-0.4735905280685486
1201	25	-0.5171944479506436
1202	1	-0.1856271416321853
1202	2	-0.028709823178477478
1202	3	-0.3744868199156848
1202	4	-0.047814673064490165
1202	5	-0.6732464362725139
1202	6	-0.6173160447338937
1202	7	-0.40845410404899596
1202	8	-0.37849006982087374
1202	9	0.034147387904141624
1202	10	0.05087160665936352
1202	11	0.008627813674067397
1202	12	-0.3234864158202768
1202	13	-0.34730591159993907
1202	14	-0.20933069383495706
1202	15	-0.31117137763065017
1202	16	-0.2379009667162748
1202	17	-0.22240890690154203
1202	18	-0.027999545887183785
1202	19	-0.22790041752875823
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2211	9	-1.1051075974182574
2211	10	0.19433248326630803
2211	11	0.4053633132185581
2211	12	0.09280824963168754
2211	13	0.49316883280739177
2211	14	0.7310149889264537
2211	15	0.5668577751176045
2211	16	0.4694530164804007
2211	17	0.7291727309160478
2211	18	0.8874600147970924
2211	19	0.7512877594122287
2211	20	0.9226193744689439
2211	21	0.6625843772945998
2211	22	0.787231150395769
2211	23	-0.9979734178095114
2211	24	0.1450683156123489
2211	25	0.08800042364757132
2212	1	0.06869797994957239
2212	2	-0.8995740502373941
2212	3	-0.0032837584471359094
2212	4	0.3850961138285989
2212	5	0.4094690225491391
2212	6	0.49267558406304346
2212	7	0.39200232583221606
2212	8	0.6081957310242174
2212	9	1.0092629902321377
2212	10	0.8644130241830865
2212	11	0.5968543145111909
2212	12	0.6079494448191198
2212	13	0.5278934027586644
2212	14	0.9414007898611274
2212	15	0.5268458431299337
2212	16	0.7724534142133237
2212	17	0.8287650546706473
2212	18	1.0362394718916548
2212	19	0.5904843389090928
2212	20	0.7474826537768848
2212	21	0.38272954981125196
2212	22	0.30867144074080244
2212	23	0.643413178591004
2212	24	0.4641768967001454
2212	25	0.6519111326065072
2213	1	0.7067759356023515
2213	2	0.3118433704646044
2213	3	0.3864075013718953
2213	4	0.45658860661968603
2213	5	0.9566376613082141
2213	6	0.7330255890347104
2213	7	0.5626993527786155
2213	8	0.6386397003987838
2213	9	0.9544429643794956
2213	10	1.0055684383224914
2213	11	0.590756571645521
2213	12	0.6826958900811277
2213	13	0.4560901811219935
2213	14	-0.6296591683769179
2213	15	0.09831844927490607
2213	16	0.4750709940084974
2213	17	1.028049135410427
2213	18	0.7751004642352939
2213	19	0.654658545929081
2213	20	0.6405969025588263
2213	21	0.45696639976650744
2213	22	0.8040850829800377
2213	23	1.1323636608421737
2213	24	0.9394503380842565
2213	25	1.143056603422849
2214	1	0.1991180819513083
2214	2	-0.1716047112328205
2214	3	-0.115921591817429
2214	4	0.3695035563266309
2214	5	0.08873051790261854
2214	6	-0.0057218195291923735
2214	7	-0.365894745145674
2214	8	-0.39811134257426417
2214	9	-0.47874021496068053
2214	10	-0.5247558431138657
2214	11	-0.5146899231971958
2214	12	-0.6213352327465458
2214	13	-0.5513066093364003
2214	14	-0.20880482628175656
2214	15	-0.4253282452043692
2214	16	-0.4209593097973965
2214	17	-0.6273500097355864
2214	18	-0.2931419662118415
2214	19	-0.47321634849389227
2214	20	-0.25002273860725666
2214	21	-0.32310833597099275
2214	22	0.19736910437698185
2214	23	-0.06303701996967703
2214	24	-0.09945575594967693
2214	25	-0.13094713336402464
2215	1	0.4643544726351436
2215	2	0.4437652028679757
2215	3	0.4071596224450431
2215	4	0.9479259655332903
2215	5	0.8984196175363977
2215	6	0.8629422748101447
2215	7	0.6074418939898578
2215	8	0.8973189024166714
2215	9	0.6881278990275135
2215	10	0.7072882273617012
2215	11	0.7486688751437001
2215	12	0.3221975334351015
2215	13	0.5018238303869467
2215	14	0.48259337308910943
2215	15	0.45261792187293537
2215	16	0.6457326669173611
2215	17	0.5908303527662895
2215	18	0.6803031445583656
2215	19	0.39292448901043286
2215	20	-0.2535558147078518
2215	21	0.012593294719245307
2215	22	-0.6197218929938728
2215	23	0.3764590518075508
2215	24	0.26963964227034865
2215	25	0.5832869277623232
2216	1	0.17672763738925923
2216	2	-0.1278809640162848
2216	3	0.28184754475201146
2216	4	0.6372340119841944
2216	5	0.5118212071284631
2216	6	0.42107461865676754
2216	7	0.3294694255411983
2216	8	0.06861233561486557
2216	9	0.6560268431169334
2216	10	0.4633522691400316
2216	11	0.0867893085022331
2216	12	-0.1840325680330963
2216	13	0.036570388943026444
2216	14	0.037988595150991955
2216	15	0.11382179736252596
2216	16	0.19960472078940938
2216	17	0.1348991587398558
2216	18	0.8071727476184449
2216	19	0.5809842246892174
2216	20	-0.7641950872518848
2216	21	-0.9352983198390348
2216	22	-1.302284480318363
2216	23	-1.3634685690724524
2216	24	-1.3522481700936524
2216	25	-1.2551312677987276
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1110.0
3	122.0
4	128.0
5	105.0
6	118.0
7	125.0
8	84.0
9	236.0
10	591.0
11	1344.0
12	2466.0
13	3984.0
14	5525.0
15	7326.0
16	8657.0
17	9447.0
18	9361.0
19	8689.0
20	8306.0
21	7788.0
22	7304.0
23	7002.0
24	7182.0
25	7150.0
26	7273.0
27	7430.0
28	8145.0
29	9277.0
30	11075.0
31	13503.0
32	17035.0
33	21785.0
34	28586.0
35	39081.0
36	59655.0
37	94649.0
38	132059.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.60584044579248	24.186701826541	12.0843981674905	20.123059560176017
2	14.583903083078114	28.412748146766326	35.006510994434414	21.996837775721147
3	18.819429594540228	33.83017589132648	27.383303915421386	19.967090598711895
4	12.136647403873344	25.183081128504824	30.990740807877202	31.68953065974463
5	10.96155868074816	45.8794226732361	29.446601624955132	13.712417021060608
6	26.25577354128048	40.73886832248841	18.151886944593937	14.853471191637176
7	21.713990613731497	41.93813760487071	18.765651330929387	17.58222045046841
8	21.89817533328984	40.531415149735764	20.193485715114214	17.37692380186019
9	24.6043308870164	26.093322460813628	17.741415239648457	31.56093141252152
10	15.111296403712297	35.08283787703016	26.417125870069608	23.388739849187935
11	26.78793937813324	34.29377119012432	18.600763568924048	20.317525862818396
12	22.044623070413707	34.96041618723352	23.243100239731675	19.751860502621103
13	25.998637809481718	30.342069225362827	24.91594994674415	18.74334301841131
14	23.151773178133066	32.02500113229766	23.13927261198424	21.683953077585034
15	25.612529881164036	33.6144292267235	22.21418745888225	18.55885343323021
16	20.884045553597208	34.43600523862745	23.827422367055338	20.852526840720003
17	18.70410241911514	36.38876202489434	23.650348332282174	21.256787223708344
18	18.24317224036033	32.388239258757324	29.816754780428234	19.55183372045411
19	20.201355781096257	35.52030500544523	24.802168669895753	19.476170543562755
20	20.423814621405192	32.785949887788206	27.653188571966798	19.137046918839808
21	22.9449882757146	34.053884598239364	23.706795011651803	19.294332114394233
22	20.863536085723293	32.91438900782826	24.10442273348041	22.11765217296804
23	22.827236508511408	31.29065555957986	24.39713871785585	21.484969214052878
24	20.795372405084848	31.811454130758904	24.322409885632606	23.07076357852364
25	21.904363373530643	33.6758890416592	24.64788859965183	19.771858985158335
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	28375.0
1	28375.0
2	17371.0
3	6367.0
4	6367.0
5	6367.0
6	6400.5
7	6434.0
8	6434.0
9	6434.0
10	6341.0
11	6248.0
12	6248.0
13	6248.0
14	6726.0
15	7204.0
16	7204.0
17	7204.0
18	9304.0
19	11404.0
20	11404.0
21	11404.0
22	14201.5
23	16999.0
24	16999.0
25	16999.0
26	21978.5
27	26958.0
28	26958.0
29	26958.0
30	28409.0
31	29860.0
32	29860.0
33	29860.0
34	31562.5
35	33265.0
36	33265.0
37	33265.0
38	37223.0
39	41181.0
40	41181.0
41	41181.0
42	64181.5
43	87182.0
44	87182.0
45	87182.0
46	77021.5
47	66861.0
48	66861.0
49	66861.0
50	62443.0
51	58025.0
52	58025.0
53	58025.0
54	51071.0
55	44117.0
56	44117.0
57	44117.0
58	38411.0
59	32705.0
60	32705.0
61	32705.0
62	29118.5
63	25532.0
64	25532.0
65	25532.0
66	18946.0
67	12360.0
68	12360.0
69	12360.0
70	9884.5
71	7409.0
72	7409.0
73	7409.0
74	5357.5
75	3306.0
76	3306.0
77	3306.0
78	2351.0
79	1396.0
80	1396.0
81	1396.0
82	898.5
83	401.0
84	401.0
85	401.0
86	240.0
87	79.0
88	79.0
89	79.0
90	52.0
91	25.0
92	25.0
93	25.0
94	15.5
95	6.0
96	6.0
97	6.0
98	5.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14448178897351105
2	0.28173948849834657
3	0.34043521526883547
4	0.3675255507013688
5	0.37149879989814033
6	0.33086329674934034
7	0.33104389898555725
8	0.34874291813481234
9	0.33465594370989504
10	0.36535832386676614
11	0.28155888626212966
12	0.3315857056942079
13	0.29817429199408346
14	0.3120806641827839
15	0.349284724843463
16	0.29799368975786655
17	0.34874291813481234
18	0.31876294692280877
19	0.33303052358394303
20	0.2929368271437937
21	0.33537835265476257
22	0.289144180183239
23	0.2714451610339839
24	0.30882982393087993
25	0.3026893478995057
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	553703.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.506447618638088
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.6646656966158	24.607923987487627
2	6.485512779743671	3.956999117905165
3	2.7434100139472477	2.5107508166078674
4	1.7676371544795528	2.1569732104755586
5	1.2066077447892476	1.840465798132811
6	0.9141423254785215	1.6732340980914318
7	0.6659048472680769	1.4220073939526547
8	0.5797392879606165	1.414862897650967
9	0.5073717368065381	1.3930298380859478
>10	3.9642488538900325	23.493509500110797
>50	0.30724367711615047	6.384116381633776
>100	0.16136247383425734	9.58489356142006
>500	0.01607670403514741	3.165174641494581
>1k	0.014290403586797697	8.527171212070106
>5k	5.954334827832375E-4	1.1369198324886416
>10k+	0.001190866965566475	6.731967712392003
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAA	26526	4.790654917889194	No Hit
GTACATAAGCAGTGGTATCAACGCA	10535	1.902644558544924	No Hit
GCTTATGTACTCTGCGTTGATACCA	6259	1.1303893964815073	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4849	0.875740243415694	No Hit
CTTATGTACTCTGCGTTGATACCAC	4337	0.783271898472647	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3334	0.6021278555471075	No Hit
GAGTACATAAGCAGTGGTATCAACG	3015	0.5445157421939198	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3010	0.5436127310128355	No Hit
TATGTACTCTGCGTTGATACCACTG	2543	0.45927148669954837	No Hit
GCGTTGATACCACTGCTTATGTACT	2194	0.39624130625985415	No Hit
CATAAGCAGTGGTATCAACGCAGAG	1916	0.346033884591559	No Hit
GTGGTATCAACGCAGAGTACATGGG	1811	0.3270706497887857	No Hit
GTACTGGTTCACTATCGGTCAGTCA	1720	0.3106358462930488	No Hit
CTGCTTATGTACTCTGCGTTGATAC	1718	0.31027464182061504	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	1628	0.29402044056109505	No Hit
ATACAGGGTGACAGCCCCGTACACA	1448	0.26151203804205503	No Hit
ACTCTGCGTTGATACCACTGCTTAT	1435	0.2591642089712355	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1414	0.25537156201068084	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1398	0.2524819262312106	No Hit
GGATACCACGTGTCCCGCCCTACTC	1343	0.2425488032392817	No Hit
GTATCAACGCAGAGTACATAAGCAG	1306	0.23586652049925683	No Hit
TATCAACGCAGAGTACATAAGCAGT	1221	0.22051533042082125	No Hit
GGTATCAACGCAGAGTACATAAGCA	1183	0.2136524454445795	No Hit
CCCATGTACTCTGCGTTGATACCAC	1048	0.1892711435552995	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	1038	0.18746512119313063	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	1031	0.18620090553961238	No Hit
CATGTACTCTGCGTTGATACCACTG	1004	0.1813246451617564	No Hit
ACATAAGCAGTGGTATCAACGCAGA	925	0.16705706850062219	No Hit
GTGGTATCAACGCAGAGTACATAAG	893	0.16127779694168173	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	841	0.15188648065840352	No Hit
CCCATATTCAGACAGGATACCACGT	767	0.13852191517835374	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	753	0.1359934838713173	No Hit
CCACTGCTTATGTACTCTGCGTTGA	722	0.13039481454859375	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	716	0.1293112011312924	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	701	0.12660216758803908	No Hit
GCCTTGGAGGATGGTCCCCCCATAT	679	0.12262891839126752	No Hit
GTACATGATAAGCAGTGGTATCAAC	678	0.12244831615505063	No Hit
ATCATGTACTCTGCGTTGATACCAC	675	0.12190650944639997	No Hit
GATACCACTGCTTATGTACTCTGCG	656	0.11847506695827907	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	638	0.11522422670637508	No Hit
TCTAAGTACCCCGAGGAAAAGAAAT	634	0.11450181776150753	No Hit
GTTCACTATCGGTCAGTCAGGAGTA	618	0.11161218198203729	No Hit
GCCCAGAGCCTGAATCAGTGTGTGT	588	0.10619411489553063	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	581	0.1049298992420124	No Hit
ATACCACTGCTTATGTACTCTGCGT	563	0.10167905899010841	No Hit
GTTGATACCACTGCTTATGTACTCT	563	0.10167905899010841	No Hit
CGGTACTGGTTCACTATCGGTCAGT	557	0.10059544557280709	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.001083613417301333	0.0
2	0.0	0.0	0.0	0.001083613417301333	0.0
3	0.0	0.0	0.0	0.001083613417301333	0.0
4	0.0	0.0	0.0	0.001083613417301333	0.0
5	0.0	0.0	0.0	0.001083613417301333	0.0
6	0.0	0.0	0.0	0.001264215653518222	0.0
7	0.0	0.0	0.0	0.001264215653518222	0.0
8	0.0	0.0	0.0	0.001264215653518222	0.0
9	0.0	0.0	0.0	0.001264215653518222	0.0
10	1.8060223621688883E-4	0.0	0.0	0.0014448178897351106	0.0
11	1.8060223621688883E-4	0.0	0.0	0.0014448178897351106	0.0
12	1.8060223621688883E-4	0.0	0.0	0.0018060223621688884	0.0
13	1.8060223621688883E-4	0.0	0.0	0.0019866245983857773	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACACCT	25	0.006013652	19.007435	5
TAATCAT	30	7.6938345E-4	19.007433	5
GCACATC	25	0.0060357307	18.99538	17
CAGGACC	35	0.0021608472	16.293564	4
TTGTCCT	45	6.727927E-4	14.784899	4
TATGACT	40	0.00525656	14.256868	4
TCATGGT	40	0.00525656	14.256868	4
CATGTTA	40	0.005275564	14.249118	10
CGAACCG	135	0.0	14.073202	12
CGGGGGA	135	0.0	14.070652	17
AACCGGG	140	0.0	13.570588	14
GCGAACC	140	0.0	13.570588	11
TAAGACA	50	0.0014919823	13.30641	4
GTCTAGA	65	5.4201315E-5	13.158993	2
GGTCTAG	60	4.064914E-4	12.673921	1
ACCGGGG	150	1.8189894E-12	12.665882	15
GGCGAAC	155	1.8189894E-12	12.257305	10
CCGGGGG	155	1.8189894E-12	12.255084	16
CACACCC	55	0.003055169	12.09564	5
CGTCTCC	55	0.0030655584	12.090159	12
>>END_MODULE