FastQCFastQC Report
Wed 25 May 2016
SRR1295183_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295183_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553703
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT156922.83401029071542No Hit
GTACATAAGCAGTGGTATCAACGCA116402.1022100295645862No Hit
GGTATCAACGCAGAGTACTTTTTTT112842.0379156334713735No Hit
TATCAACGCAGAGTACTTTTTTTTT99291.793199603397489No Hit
GCTTATGTACTCTGCGTTGATACCA69591.2568109618333294No Hit
ACGCAGAGTACTTTTTTTTTTTTTT56561.0214862480427234No Hit
CTTATGTACTCTGCGTTGATACCAC52340.9452721043591963No Hit
GTACTTTTTTTTTTTTTTTTTTTTT51370.9277536874461579No Hit
GAGTACATAAGCAGTGGTATCAACG33080.5974321974054683No Hit
GAGTACTTTTTTTTTTTTTTTTTTT31200.5634789769966931No Hit
CATAAGCAGTGGTATCAACGCAGAG25060.45258920395952346No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA24930.45024137488870386No Hit
GCGTTGATACCACTGCTTATGTACT24530.4430172854400284No Hit
TATGTACTCTGCGTTGATACCACTG22520.40671623596043366No Hit
GCAGAGTACTTTTTTTTTTTTTTTT21730.39244865929929945No Hit
GTGGTATCAACGCAGAGTACATGGG21290.38450216090575634No Hit
ACGCAGAGTACATAAGCAGTGGTAT19970.360662665725127No Hit
CTGCTTATGTACTCTGCGTTGATAC19640.3547027919299697No Hit
GTACTGGTTCACTATCGGTCAGTCA18070.3263482408439181No Hit
ATCAACGCAGAGTACTTTTTTTTTT17610.31804053797794124No Hit
ATACAGGGTGACAGCCCCGTACACA17590.3176793335055075No Hit
ACTCTGCGTTGATACCACTGCTTAT15760.28462912427781684No Hit
GTGGTATCAACGCAGAGTACTTTTT15560.28101707955347904No Hit
GTATCAACGCAGAGTACATAAGCAG15100.2727093766875021No Hit
GGATACCACGTGTCCCGCCCTACTC14560.26295685593179013No Hit
TATCAACGCAGAGTACATAAGCAGT14020.25320433517607815No Hit
GGTATCAACGCAGAGTACATAAGCA13550.24471603007388437No Hit
CCCATGTACTCTGCGTTGATACCAC12890.2327962824835697No Hit
CATGTACTCTGCGTTGATACCACTG11310.20426112916130126No Hit
ACATAAGCAGTGGTATCAACGCAGA11040.1993848687834453No Hit
ACCCTGTATCGCGCGCCTTTCCAGA10450.18872933684664883No Hit
GTGGTATCAACGCAGAGTACATAAG9810.17717079372876796No Hit
AAGCAGTGGTATCAACGCAGAGTAC9360.16904369309900796No Hit
CCCATATTCAGACAGGATACCACGT8840.15965237681572975No Hit
CCACTGCTTATGTACTCTGCGTTGA8120.14664901580811374No Hit
ATCATGTACTCTGCGTTGATACCAC7970.1439399822648604No Hit
GATACCACTGCTTATGTACTCTGCG7700.1390637218870044No Hit
CAACGCAGAGTACTTTTTTTTTTTT7520.1358128816351004No Hit
AACGCAGAGTACTTTTTTTTTTTTT7180.12967240560372617No Hit
GCCTTGGAGGATGGTCCCCCCATAT7130.12876939442264174No Hit
GTACATGATAAGCAGTGGTATCAAC6850.12371253180856887No Hit
GCCCAGAGCCTGAATCAGTGTGTGT6650.12010048708423107No Hit
ATACCACTGCTTATGTACTCTGCGT6520.11775265801341152No Hit
GTATTTAGCCTTGGAGGATGGTCCC6400.11558543117880886No Hit
GTTCACTATCGGTCAGTCAGGAGTA6210.11215398869068798No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA6180.11161218198203729No Hit
CGGTACTGGTTCACTATCGGTCAGT6010.1085419439663502No Hit
GTCAGGAGTATTTAGCCTTGGAGGA6010.1085419439663502No Hit
TCTAAGTACCCCGAGGAAAAGAAAT5960.10763893278526575No Hit
CTATCGGTCAGTCAGGAGTATTTAG5950.10745833054904887No Hit
CACACACACTGATTCAGGCTCTGGG5920.10691652384039818No Hit
TCACGGTACTGGTTCACTATCGGTC5790.10456869476957863No Hit
GCAGTGGTATCAACGCAGAGTACAT5750.10384628582471107No Hit
TATTCAGACAGGATACCACGTGTCC5730.1034850813522773No Hit
GGTATCAACGCAGAGTACATGGGGG5700.10294327464362664No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG5690.10276267240740976No Hit
GTACTCTGCGTTGATACCACTGCTT5600.10113725228145774No Hit
ATCATTAACTGAATCCATAGGTTAA5590.10095665004524086No Hit
GTTGATACCACTGCTTATGTACTCT5590.10095665004524086No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCT401.2815608E-519.0020778
CTAAGAG250.00602661919.000363
GACCGAT250.006032910718.9969318
TGACCGA250.006032910718.9969317
ATAACCT359.93726E-518.9969319
CACCGTC504.550784E-617.1003257
CCGTCTG453.5289384E-516.8907369
TGCACCG453.534411E-516.8876865
AATAACC402.7604934E-416.62231318
CTCCTAT350.002166244716.28749510
ATAGATC350.002167552216.2860243
TAGATCC350.002168860816.2845544
TTTGGCA350.002170169716.28308318
GCACCGT551.1264541E-515.5443466
CGTCTGC508.6896915E-515.20166210
ACGCTTC456.751631E-414.7780597
CAATTTG456.7608856E-414.77539115
CATCTAC400.00526013314.2554211
AGATCCT400.00527592214.2489835
TTGGCAT400.00527908414.24769719
Produced by FastQC (version 0.11.5)