##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295183_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	553703
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.600692067769184	31.0	30.0	33.0	26.0	34.0
2	30.589285230529725	31.0	30.0	33.0	26.0	34.0
3	30.748236870668933	31.0	30.0	34.0	27.0	34.0
4	34.31718990144536	35.0	35.0	37.0	30.0	37.0
5	34.629685950771446	37.0	35.0	37.0	31.0	37.0
6	34.250527810035344	37.0	35.0	37.0	30.0	37.0
7	34.184306388081694	37.0	35.0	37.0	29.0	37.0
8	34.16745078137558	37.0	35.0	37.0	29.0	37.0
9	35.6062817069801	39.0	35.0	39.0	28.0	39.0
10	35.678807953000074	39.0	35.0	39.0	29.0	39.0
11	35.69144649749053	39.0	35.0	39.0	29.0	39.0
12	35.65496123373	39.0	35.0	39.0	29.0	39.0
13	35.43575526952175	39.0	35.0	39.0	27.0	39.0
14	36.43243218837536	40.0	36.0	41.0	27.0	41.0
15	36.40930426600542	40.0	36.0	41.0	27.0	41.0
16	36.324684894248364	39.0	36.0	41.0	27.0	41.0
17	36.50497649461896	39.0	36.0	41.0	27.0	41.0
18	36.56179576415515	40.0	36.0	41.0	27.0	41.0
19	36.6926366662272	40.0	36.0	41.0	28.0	41.0
20	36.69437405973961	40.0	36.0	41.0	28.0	41.0
21	36.658085652416545	40.0	36.0	41.0	27.0	41.0
22	36.61993343001573	40.0	36.0	41.0	27.0	41.0
23	36.53783165343153	40.0	36.0	41.0	27.0	41.0
24	36.5609198433095	40.0	36.0	41.0	27.0	41.0
25	35.22195292422111	39.0	33.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.11718318664117078
1101	2	0.16684394969778893
1101	3	0.055652763262493465
1101	4	-0.09292567294977516
1101	5	-0.09311325390328307
1101	6	-0.05832937509821079
1101	7	0.1252299182642176
1101	8	0.10291445345558259
1101	9	0.14649134106559814
1101	10	0.24710995389036583
1101	11	0.22621188123651592
1101	12	0.09244769356907767
1101	13	0.1959652058535184
1101	14	0.5275532444636042
1101	15	0.5260706733972711
1101	16	0.5215277078858023
1101	17	0.5123875584434003
1101	18	0.3471725286471923
1101	19	0.38898052077507117
1101	20	0.5898544200287148
1101	21	0.36651074028431907
1101	22	0.3669862608278365
1101	23	0.14969808054127043
1101	24	0.32570255013639837
1101	25	0.2644044867784814
1102	1	-0.1531649779232751
1102	2	0.010627533360636932
1102	3	-0.14237579538454526
1102	4	-0.18674653608525915
1102	5	-0.20915268273034116
1102	6	-0.0627592048467136
1102	7	0.05711389426137714
1102	8	-0.12493254130472309
1102	9	0.15993766119600394
1102	10	0.007014724120260496
1102	11	0.2775855655282342
1102	12	0.06719020810806597
1102	13	0.4215111573027883
1102	14	0.2704251521956209
1102	15	0.004602839154664196
1102	16	0.2943491726348455
1102	17	0.21945060445911935
1102	18	0.1408653560766595
1102	19	0.03261190134963954
1102	20	0.4016547871672458
1102	21	0.2102274779962343
1102	22	0.008902785661106805
1102	23	0.16008491291733407
1102	24	0.15011623401675678
1102	25	0.6646986089625955
1103	1	-0.19432922870871394
1103	2	-0.1988445378868633
1103	3	-0.04931337669236058
1103	4	-0.3434900070355553
1103	5	-0.3967249920522704
1103	6	-0.08428874304403422
1103	7	-0.26754660543781483
1103	8	0.01262099973097719
1103	9	-0.18533710137232617
1103	10	-0.03279975265591162
1103	11	0.03320962390017712
1103	12	-0.22922273532483217
1103	13	-0.03992644200201312
1103	14	0.0523839442378673
1103	15	0.05203004134310163
1103	16	0.01588436702801488
1103	17	-0.1952873851231942
1103	18	-0.16637148941149604
1103	19	-0.07603076590664415
1103	20	-0.21827199080649962
1103	21	-0.057868492221324175
1103	22	-0.1318850710006032
1103	23	-0.18438769823976742
1103	24	-0.10093401871235841
1103	25	0.10639094276042727
1104	1	-0.3563365216415164
1104	2	-0.4348165272195885
1104	3	-0.7072686083479383
1104	4	-0.475190421442079
1104	5	-0.4114598781358296
1104	6	-0.4198880515889982
1104	7	-0.30747502377329994
1104	8	-0.3004645535755941
1104	9	-0.11885193733316868
1104	10	-0.043942597210701706
1104	11	-0.1419581213292247
1104	12	-0.07526773173538004
1104	13	-0.26737575560071747
1104	14	-0.057317159550365204
1104	15	-0.3173178709447413
1104	16	-0.40240187151360374
1104	17	-0.44556015739657084
1104	18	-0.14322148150693437
1104	19	-0.24109621829394712
1104	20	-0.10210728559492566
1104	21	-0.26082143025730886
1104	22	-0.28828919381421514
1104	23	-0.04823610683060764
1104	24	0.09012967652909509
1104	25	-0.4514575124803031
1105	1	-0.18139146669646067
1105	2	-0.28797578587138517
1105	3	-0.10614149665366313
1105	4	-0.200085943830139
1105	5	-0.293500225847815
1105	6	-0.39715556019979203
1105	7	-0.392465018852846
1105	8	-0.451735014457995
1105	9	-0.24895657896019685
1105	10	-0.22843857884101482
1105	11	-0.3866213983507123
1105	12	-0.23668613422840679
1105	13	-0.38698867998976283
1105	14	-0.31935291483984685
1105	15	-0.21469166133345396
1105	16	-0.47619947689329933
1105	17	-0.48449602749662546
1105	18	-0.47175813887941587
1105	19	-0.4085634972836161
1105	20	-0.525093016282149
1105	21	-0.262401223598296
1105	22	-0.22678091105220233
1105	23	-0.15997105844615334
1105	24	-0.3572706843041047
1105	25	-0.15644685149948856
1106	1	-0.3779159096054272
1106	2	-0.5646135471427591
1106	3	-0.6478350533992163
1106	4	-0.35291786644198453
1106	5	-0.3187895677030568
1106	6	-0.35435023976447866
1106	7	-0.48035446435292783
1106	8	-0.3730423214194687
1106	9	-0.3664248809121773
1106	10	-0.4396211665072798
1106	11	-0.4468141777599328
1106	12	-0.5738745951024811
1106	13	-0.8716388786423792
1106	14	-1.043902232511293
1106	15	-0.897459669625043
1106	16	-0.907478108781369
1106	17	-0.5873282955912842
1106	18	-0.7621267690400586
1106	19	-0.7116251659656001
1106	20	-0.908349537270233
1106	21	-0.7914072695475127
1106	22	-0.42978522958735255
1106	23	-0.6305227944972884
1106	24	-0.4718673331208265
1106	25	-0.9091087965677218
1107	1	0.05068952317980546
1107	2	0.1880017169407644
1107	3	0.10256041560730367
1107	4	0.0863662496456783
1107	5	0.15711844681809595
1107	6	0.3618995579104478
1107	7	0.42576670218202395
1107	8	0.3945551992652909
1107	9	0.35214128380695797
1107	10	0.3959303750397538
1107	11	0.16809626194175564
1107	12	0.18335436070972122
1107	13	0.235463513486053
1107	14	0.22753572408028333
1107	15	0.3329528830984856
1107	16	0.30729658609882193
1107	17	0.12214514235714802
1107	18	0.307157496666143
1107	19	0.09084707639467382
1107	20	-0.027311239004312426
1107	21	0.22037042994500666
1107	22	0.1433068279804175
1107	23	-0.030189281168311766
1107	24	0.06860108172514146
1107	25	0.322563272235044
1108	1	0.04075061320405382
1108	2	0.0285434120145176
1108	3	0.06186596487284035
1108	4	0.18922417586518492
1108	5	0.17349806421902514
1108	6	0.22177806330402206
1108	7	0.12191023375091703
1108	8	0.019078602827875102
1108	9	0.0866989472328683
1108	10	-0.013700820407933634
1108	11	-0.035281743446311964
1108	12	-0.20572826076346473
1108	13	0.06348854680955895
1108	14	-0.28949934729748605
1108	15	-0.15020666211069766
1108	16	0.15159719650946357
1108	17	0.08999301338632648
1108	18	0.1161529445964149
1108	19	0.37870092056186877
1108	20	0.11187511678173934
1108	21	0.09109605605350168
1108	22	0.1500535363284925
1108	23	0.39440046339435497
1108	24	0.4136293623304397
1108	25	0.42189606782275035
1109	1	0.4271018978624781
1109	2	0.21910930390222916
1109	3	0.35486084526671036
1109	4	0.36402334578735207
1109	5	0.4457659724347778
1109	6	0.6629537294176941
1109	7	0.5034225689691709
1109	8	0.4197778661040701
1109	9	0.4502659034753975
1109	10	0.5573974867327536
1109	11	0.627567516559516
1109	12	0.5939649196357593
1109	13	0.4328708047877612
1109	14	0.6048750320766203
1109	15	0.6791600170428111
1109	16	0.6464484544103684
1109	17	0.7765625205127478
1109	18	0.7527726964055219
1109	19	0.6716113661043579
1109	20	0.6790537440613278
1109	21	0.6864949512650895
1109	22	0.7652815401577939
1109	23	0.6791421343719577
1109	24	0.6505357169449226
1109	25	0.6146382382741251
1110	1	0.22278435747936243
1110	2	0.2893428954472981
1110	3	0.18599037147820496
1110	4	0.27973823199403824
1110	5	0.33726900492141
1110	6	0.5841631211188911
1110	7	0.4201943218064983
1110	8	0.3909902602672801
1110	9	0.5031752891716721
1110	10	0.27393504753261055
1110	11	0.4489390412097265
1110	12	0.3765446350023609
1110	13	0.4585058255047443
1110	14	0.24433939285245998
1110	15	0.4750226243808271
1110	16	0.47580052310669885
1110	17	0.42293254393194957
1110	18	0.557516586395316
1110	19	0.5018101290900105
1110	20	0.46976412657499367
1110	21	0.3661262489291772
1110	22	0.3232410669697785
1110	23	0.3932597107846121
1110	24	0.32185019481677557
1110	25	0.5365201814675444
1111	1	0.1813506234281803
1111	2	0.28558893129065055
1111	3	0.43470094502099244
1111	4	0.4052563380490568
1111	5	0.19982032384857007
1111	6	0.4963567440507717
1111	7	0.37221634899631084
1111	8	0.3076679896966894
1111	9	0.46885614075856097
1111	10	0.3453242846936604
1111	11	0.4475697170216151
1111	12	0.4237408392455464
1111	13	0.4162903228865602
1111	14	0.19171260129949275
1111	15	0.3290334904722343
1111	16	0.10034510604054248
1111	17	0.3761444327143053
1111	18	0.33390373392339967
1111	19	0.6304700597633612
1111	20	0.436878220534723
1111	21	0.20791644977978052
1111	22	-0.01623824566389942
1111	23	0.0019132496394931309
1111	24	0.1256631603747067
1111	25	0.1061737940741736
1112	1	0.09306949402956022
1112	2	0.04119586175809431
1112	3	0.050513799857053954
1112	4	0.2493686016244112
1112	5	0.43931154069888834
1112	6	0.2059112525055795
1112	7	0.2058375941967583
1112	8	0.3322875172271864
1112	9	0.08888476042331206
1112	10	0.22707949344814438
1112	11	0.20103874873112204
1112	12	0.1892102826077675
1112	13	0.285807439553345
1112	14	0.32334713210116206
1112	15	0.22033634680298775
1112	16	0.3221156537591767
1112	17	0.13305615526037684
1112	18	0.2396585408264258
1112	19	0.1421396790581113
1112	20	0.10470388561113708
1112	21	0.21178059458352294
1112	22	-0.011610828123082229
1112	23	0.2151101495025216
1112	24	-0.03034219111054881
1112	25	0.2373819702811346
1113	1	-0.001202668710305943
1113	2	-0.11406515025149844
1113	3	0.12849412323305742
1113	4	0.13893608260158175
1113	5	-0.09567140268859475
1113	6	-0.06059168354780553
1113	7	-0.20850781306303645
1113	8	-0.10123029450834053
1113	9	-0.16184141218864312
1113	10	-0.23173373302603295
1113	11	-0.14883411074844588
1113	12	-0.012195259464405694
1113	13	-0.18931089521946376
1113	14	-0.30054611811235077
1113	15	-0.2775261484322584
1113	16	-0.2934254051500673
1113	17	-0.04648218231097445
1113	18	-0.3729500834986723
1113	19	-0.011127373935600815
1113	20	-0.08948697474659184
1113	21	-0.08657743505204252
1113	22	0.07303532557460812
1113	23	-0.00567527493451081
1113	24	-0.09367722930095823
1113	25	-0.5092851145580184
1114	1	0.39827107548576635
1114	2	0.3994752827450405
1114	3	0.16780226037670687
1114	4	0.7556575882283596
1114	5	0.4900768270205731
1114	6	0.3259040573480192
1114	7	0.1299286819345582
1114	8	-0.009269322669467783
1114	9	0.1507914525358629
1114	10	-0.011268725072731911
1114	11	0.05673518207673567
1114	12	-0.016870611168791072
1114	13	0.11847813665816176
1114	14	0.183666207769825
1114	15	-0.044999872807025554
1114	16	0.13406823964213288
1114	17	0.1390180332457831
1114	18	0.31390665302159704
1114	19	0.3045703609841013
1114	20	0.3922613144265128
1114	21	0.25861564929259373
1114	22	0.1714283127948164
1114	23	0.07779722051897409
1114	24	0.2463288657521403
1114	25	0.008727501706360385
1115	1	0.18266168944971994
1115	2	0.05868866595585942
1115	3	0.15905451663537207
1115	4	0.41321638175126196
1115	5	0.14686522265716917
1115	6	-0.048318350897460505
1115	7	-0.11066435440101685
1115	8	-0.10715694136829512
1115	9	-0.33970740952498346
1115	10	-0.133833927678225
1115	11	-0.04344531861649159
1115	12	-0.09949676545764419
1115	13	-0.07340064012264946
1115	14	0.046779975525602424
1115	15	-0.13876475103445074
1115	16	-0.11074432739164308
1115	17	-0.11045616038698824
1115	18	-0.2327083049923715
1115	19	-0.2768890786789626
1115	20	-0.0023288966808223677
1115	21	-0.07151750266806545
1115	22	-0.02288722334456139
1115	23	-0.1960707262202419
1115	24	-0.1996294882908174
1115	25	0.11584550730449195
1116	1	0.432570173515181
1116	2	0.40881786492226624
1116	3	0.447851889589721
1116	4	0.7374788709846811
1116	5	0.6422140598664683
1116	6	0.6130296249853942
1116	7	0.7583709599831323
1116	8	0.2630573135843335
1116	9	0.35594289193927153
1116	10	0.20404290264046665
1116	11	0.2835518820725724
1116	12	0.20936413032185186
1116	13	0.15294518244409971
1116	14	0.15361137087443666
1116	15	0.47707288893109023
1116	16	0.21941824797442422
1116	17	0.3207830730324801
1116	18	0.2984124960412231
1116	19	0.2955753916052686
1116	20	0.7366622747231375
1116	21	0.4040154430683671
1116	22	0.5578513640800722
1116	23	0.25627100504591027
1116	24	0.19815259611917924
1116	25	-1.4120883858910247
1201	1	-0.08084864205807207
1201	2	0.04524183155101014
1201	3	0.04582859923452176
1201	4	-0.015084806173710774
1201	5	-0.1106300812029275
1201	6	-0.069738040290801
1201	7	-0.05347330749253132
1201	8	-0.0020837874142713986
1201	9	0.2444209604514711
1201	10	0.09727700705204256
1201	11	0.12179530894654533
1201	12	0.1392007502182011
1201	13	0.11109680676467093
1201	14	0.41758321387784747
1201	15	0.570732034424708
1201	16	0.3699009619170326
1201	17	0.3051363469548676
1201	18	0.17539154420201442
1201	19	0.29693820920102354
1201	20	0.2036662881222071
1201	21	0.151277957289075
1201	22	0.27216529405099976
1201	23	0.08994800542512849
1201	24	0.2813121208316005
1201	25	0.5636376601208539
1202	1	-0.28755620286119665
1202	2	-0.2052962491918464
1202	3	-0.2377399522521202
1202	4	-0.43910396184815426
1202	5	-0.1291823365299294
1202	6	-0.26021862326285117
1202	7	-0.27628869967652747
1202	8	-0.14538237610535276
1202	9	-0.09275580675942763
1202	10	-0.3100962364986728
1202	11	-0.2600229427522578
1202	12	-0.12540814195041605
1202	13	-0.153423686424766
1202	14	-0.32921520570214113
1202	15	0.06009340945161057
1202	16	-0.23369111138493537
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2210	21	0.22397571653473847
2210	22	0.3445855761191652
2210	23	0.36340176385349565
2210	24	0.3127641589014729
2210	25	0.33877646177260345
2211	1	0.04660211275779247
2211	2	0.2579439573067859
2211	3	0.13872527702210036
2211	4	0.01177922470918702
2211	5	0.033787093574332516
2211	6	-0.24434657143574157
2211	7	-0.24004768337933058
2211	8	-0.06784416373567836
2211	9	-0.2095793718975969
2211	10	-0.27828127547022774
2211	11	-0.41148817202647336
2211	12	-0.41868934450128137
2211	13	-0.282141167951238
2211	14	-0.1944925617098363
2211	15	-0.22738508027406823
2211	16	-0.32596512697355706
2211	17	-0.21446713504076342
2211	18	-0.2937613491522839
2211	19	-0.2908556321150755
2211	20	-0.18978643522275718
2211	21	-0.1828057179803224
2211	22	-0.11930739580340344
2211	23	-0.16778486261950576
2211	24	-0.1343172332607665
2211	25	-0.2184372206808689
2212	1	0.09209778741226415
2212	2	0.050533695797390976
2212	3	0.11118972964216312
2212	4	0.1994892774088015
2212	5	0.19641759968947525
2212	6	0.05726921856127376
2212	7	-0.031779810256132635
2212	8	-0.25702831407872395
2212	9	-0.16995025784768103
2212	10	-0.11372176468172057
2212	11	-0.2605012972129117
2212	12	-0.1875344275280284
2212	13	-0.13292420464210153
2212	14	-0.31679792116096195
2212	15	-0.36451593440551733
2212	16	-0.0544776058439993
2212	17	-0.2658790869150778
2212	18	-0.2008807684622198
2212	19	-0.42102367427477105
2212	20	-0.27293498846925246
2212	21	-0.1594985687310384
2212	22	0.020629961261327878
2212	23	0.12257508567520858
2212	24	-0.20237182210435378
2212	25	-0.05393736983072017
2213	1	0.33536615770161404
2213	2	0.3512023842730798
2213	3	0.19378145679737813
2213	4	0.262341022461996
2213	5	0.33828147559680843
2213	6	0.42082196789009885
2213	7	0.4318624289802244
2213	8	0.3130547126688157
2213	9	0.17131950450689715
2213	10	0.3295838930690991
2213	11	0.42446688415330414
2213	12	0.36220953190321126
2213	13	0.4796565848577501
2213	14	0.4594400225598321
2213	15	0.6029519983776197
2213	16	0.6290910528017193
2213	17	0.6203643256333962
2213	18	0.6343285384881625
2213	19	0.6304926824916635
2213	20	0.5888652501704996
2213	21	0.5834864168511444
2213	22	0.6997937277920983
2213	23	0.6277207553580197
2213	24	0.41736815999766463
2213	25	0.7141470489820492
2214	1	0.19307647993549537
2214	2	0.09035418484127433
2214	3	-0.037592196295708646
2214	4	0.11279788304913296
2214	5	0.21681542660906672
2214	6	-0.006950548810266355
2214	7	-0.27659908979329373
2214	8	-0.09076397638445144
2214	9	-0.07240858026185748
2214	10	-0.18319028726926234
2214	11	-0.27936204267221854
2214	12	-0.4920353044204049
2214	13	-0.18327244178605184
2214	14	-0.12934705737157515
2214	15	-0.5762627644899823
2214	16	-0.32865766107813954
2214	17	-0.23271340465249324
2214	18	-0.28510275326274837
2214	19	-0.41955210776198015
2214	20	-0.44686457501953925
2214	21	-0.32508003908804994
2214	22	-0.16763479976030737
2214	23	-0.2214640620257171
2214	24	-0.21776531495818574
2214	25	-0.22823988762054626
2215	1	0.21448154917655415
2215	2	0.07151627762068102
2215	3	0.1815773242080141
2215	4	0.3569752806596611
2215	5	0.28563809614765034
2215	6	-0.11098775974627273
2215	7	0.1491494936188147
2215	8	0.024655008217742136
2215	9	-0.13139648825725203
2215	10	-0.1977900527639136
2215	11	-0.1337052985774676
2215	12	-0.18429611155267622
2215	13	-0.06875738747275051
2215	14	-0.03183364046343229
2215	15	-0.06738100373708278
2215	16	-0.09834913675724977
2215	17	-0.09089965561453539
2215	18	-0.04916403457102092
2215	19	-0.26968821383690766
2215	20	-0.1665170525633286
2215	21	-0.1358796816481842
2215	22	-0.06974689744676255
2215	23	-0.01746765708561071
2215	24	-0.09559267976895569
2215	25	0.20287287639166607
2216	1	0.40391557194391225
2216	2	0.20202774146547853
2216	3	0.3061220916099252
2216	4	0.3155863345088079
2216	5	0.3967033976891372
2216	6	-0.005236368280598924
2216	7	-0.17597247436030017
2216	8	-0.18015957754922596
2216	9	-0.21336126069821404
2216	10	-0.36175395482305817
2216	11	-0.4218703962663781
2216	12	-0.1551841300597374
2216	13	-0.3990329320340109
2216	14	-0.5785364966900985
2216	15	-0.42844057908237687
2216	16	-0.5616213143151398
2216	17	-0.4302340425679674
2216	18	-0.41746152191613106
2216	19	-0.6503771897639794
2216	20	-0.49780040765620726
2216	21	-0.31796126849167194
2216	22	-0.2893007122386706
2216	23	-0.3162949321033608
2216	24	-0.47594953631629267
2216	25	-0.5797721681581436
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	30.0
9	153.0
10	288.0
11	507.0
12	674.0
13	1123.0
14	1752.0
15	2437.0
16	3305.0
17	4122.0
18	4503.0
19	4491.0
20	4484.0
21	4364.0
22	4191.0
23	4022.0
24	4118.0
25	4352.0
26	4349.0
27	4770.0
28	5398.0
29	6496.0
30	8274.0
31	10439.0
32	13934.0
33	18541.0
34	26105.0
35	37914.0
36	57093.0
37	97850.0
38	213624.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.38083797722162	19.156603654296013	13.215256640684977	21.247301727797396
2	14.701824092468845	23.22665703449521	38.932815604117756	23.13870326891819
3	18.899574675588546	30.005869657482908	30.731901136907503	20.36265453002104
4	11.40394760367923	20.363985746871517	34.375287834813975	33.856778814635284
5	10.628828233237057	41.37860650955444	32.46576604403405	15.52679921317445
6	27.882830165507176	36.785252450821034	19.276962643491327	16.05495474018046
7	22.39321364134665	38.461149469296494	20.330540600579013	18.815096288777838
8	23.83230569732959	34.61616260127362	21.90183096200456	19.64970073939223
9	26.374676265229528	18.281939121608687	20.539323164058125	34.804061449103656
10	16.729095177894166	29.122629582806574	28.65667328878454	25.49160195051472
11	28.22396688393665	28.045888576439122	20.867671201703484	22.862473337920747
12	24.581077224833024	29.196181201483885	26.18885930913412	20.033882264548968
13	28.832329056123925	23.626524478353854	28.66832119530663	18.872825270215596
14	24.68468858990823	25.66977146583005	27.565884416220094	22.079655528041627
15	27.530824286666316	26.525772842119334	26.996241667464325	18.947161203750024
16	20.727538048376115	28.329989181926052	28.850484826703127	22.09198794299471
17	18.20853094263701	29.998627420525843	28.533760036987406	23.259081599849736
18	18.021852988983202	24.643308650893985	36.50839804948528	20.82644031063753
19	20.145529807603744	28.095668962129377	32.4025060456817	19.356295184585182
20	20.174283676031028	25.35692032617235	35.53743486937754	18.931361128419073
21	22.415648963875615	26.990525521132458	31.65371736939631	18.940108145595612
22	20.044680993240057	25.952541344366924	32.07423474317459	21.928542919218426
23	22.302570150423602	24.109676126009795	32.14485021753539	21.44290350603121
24	19.993859490698934	25.577027271085424	31.87466136897237	22.554451869243273
25	21.29959400104028	26.897250476795932	32.24224122984454	19.56091429231925
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3465.0
1	3465.0
2	2877.0
3	2289.0
4	2289.0
5	2289.0
6	4835.0
7	7381.0
8	7381.0
9	7381.0
10	6696.5
11	6012.0
12	6012.0
13	6012.0
14	5453.0
15	4894.0
16	4894.0
17	4894.0
18	7724.5
19	10555.0
20	10555.0
21	10555.0
22	15142.0
23	19729.0
24	19729.0
25	19729.0
26	27111.0
27	34493.0
28	34493.0
29	34493.0
30	36960.0
31	39427.0
32	39427.0
33	39427.0
34	39815.0
35	40203.0
36	40203.0
37	40203.0
38	40526.0
39	40849.0
40	40849.0
41	40849.0
42	66170.5
43	91492.0
44	91492.0
45	91492.0
46	79715.0
47	67938.0
48	67938.0
49	67938.0
50	63551.5
51	59165.0
52	59165.0
53	59165.0
54	51854.5
55	44544.0
56	44544.0
57	44544.0
58	38671.0
59	32798.0
60	32798.0
61	32798.0
62	29427.0
63	26056.0
64	26056.0
65	26056.0
66	18683.5
67	11311.0
68	11311.0
69	11311.0
70	9170.5
71	7030.0
72	7030.0
73	7030.0
74	4880.5
75	2731.0
76	2731.0
77	2731.0
78	1885.0
79	1039.0
80	1039.0
81	1039.0
82	643.5
83	248.0
84	248.0
85	248.0
86	145.5
87	43.0
88	43.0
89	43.0
90	24.5
91	6.0
92	6.0
93	6.0
94	5.0
95	4.0
96	4.0
97	4.0
98	2.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.019505041511423994
2	5.418067086506665E-4
3	0.0014448178897351106
4	0.0
5	0.01553179231465244
6	0.0019866245983857773
7	7.224089448675553E-4
8	9.030111810844442E-4
9	0.0016254201259519995
10	5.418067086506665E-4
11	0.002528431307036444
12	0.003792646960554666
13	0.012822758771399108
14	0.00794649839354311
15	0.0
16	0.0
17	1.8060223621688883E-4
18	5.418067086506665E-4
19	3.6120447243377765E-4
20	0.0014448178897351106
21	9.030111810844442E-4
22	0.0
23	0.0
24	5.418067086506665E-4
25	0.001264215653518222
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	553703.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.354336072806433
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.21564760479282	17.799658481689164
2	7.219358284324342	3.372066392042198
3	3.307762030586636	2.3175175833356683
4	2.117383388478649	1.9780033299803212
5	1.5894804740949757	1.8560630586588882
6	1.1749417290063366	1.6463990403106952
7	0.9224024396081144	1.507946759828911
8	0.7808180743058329	1.4588390175247972
9	0.6145832888627829	1.2917866205548896
>10	5.323225179183516	24.70375144390729
>50	0.45068815414604535	7.194185680973507
>100	0.2326880521405826	10.107390362467898
>500	0.027056750248904953	4.0625267565063705
>1k	0.017780150163566114	7.78960889859674
>5k	0.0038652500355578507	5.9424970309486795
>10k+	0.00231915002133471	6.9717595426739845
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	15692	2.83401029071542	No Hit
GTACATAAGCAGTGGTATCAACGCA	11640	2.1022100295645862	No Hit
GGTATCAACGCAGAGTACTTTTTTT	11284	2.0379156334713735	No Hit
TATCAACGCAGAGTACTTTTTTTTT	9929	1.793199603397489	No Hit
GCTTATGTACTCTGCGTTGATACCA	6959	1.2568109618333294	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	5656	1.0214862480427234	No Hit
CTTATGTACTCTGCGTTGATACCAC	5234	0.9452721043591963	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5137	0.9277536874461579	No Hit
GAGTACATAAGCAGTGGTATCAACG	3308	0.5974321974054683	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3120	0.5634789769966931	No Hit
CATAAGCAGTGGTATCAACGCAGAG	2506	0.45258920395952346	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	2493	0.45024137488870386	No Hit
GCGTTGATACCACTGCTTATGTACT	2453	0.4430172854400284	No Hit
TATGTACTCTGCGTTGATACCACTG	2252	0.40671623596043366	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	2173	0.39244865929929945	No Hit
GTGGTATCAACGCAGAGTACATGGG	2129	0.38450216090575634	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	1997	0.360662665725127	No Hit
CTGCTTATGTACTCTGCGTTGATAC	1964	0.3547027919299697	No Hit
GTACTGGTTCACTATCGGTCAGTCA	1807	0.3263482408439181	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	1761	0.31804053797794124	No Hit
ATACAGGGTGACAGCCCCGTACACA	1759	0.3176793335055075	No Hit
ACTCTGCGTTGATACCACTGCTTAT	1576	0.28462912427781684	No Hit
GTGGTATCAACGCAGAGTACTTTTT	1556	0.28101707955347904	No Hit
GTATCAACGCAGAGTACATAAGCAG	1510	0.2727093766875021	No Hit
GGATACCACGTGTCCCGCCCTACTC	1456	0.26295685593179013	No Hit
TATCAACGCAGAGTACATAAGCAGT	1402	0.25320433517607815	No Hit
GGTATCAACGCAGAGTACATAAGCA	1355	0.24471603007388437	No Hit
CCCATGTACTCTGCGTTGATACCAC	1289	0.2327962824835697	No Hit
CATGTACTCTGCGTTGATACCACTG	1131	0.20426112916130126	No Hit
ACATAAGCAGTGGTATCAACGCAGA	1104	0.1993848687834453	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	1045	0.18872933684664883	No Hit
GTGGTATCAACGCAGAGTACATAAG	981	0.17717079372876796	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	936	0.16904369309900796	No Hit
CCCATATTCAGACAGGATACCACGT	884	0.15965237681572975	No Hit
CCACTGCTTATGTACTCTGCGTTGA	812	0.14664901580811374	No Hit
ATCATGTACTCTGCGTTGATACCAC	797	0.1439399822648604	No Hit
GATACCACTGCTTATGTACTCTGCG	770	0.1390637218870044	No Hit
CAACGCAGAGTACTTTTTTTTTTTT	752	0.1358128816351004	No Hit
AACGCAGAGTACTTTTTTTTTTTTT	718	0.12967240560372617	No Hit
GCCTTGGAGGATGGTCCCCCCATAT	713	0.12876939442264174	No Hit
GTACATGATAAGCAGTGGTATCAAC	685	0.12371253180856887	No Hit
GCCCAGAGCCTGAATCAGTGTGTGT	665	0.12010048708423107	No Hit
ATACCACTGCTTATGTACTCTGCGT	652	0.11775265801341152	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	640	0.11558543117880886	No Hit
GTTCACTATCGGTCAGTCAGGAGTA	621	0.11215398869068798	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	618	0.11161218198203729	No Hit
CGGTACTGGTTCACTATCGGTCAGT	601	0.1085419439663502	No Hit
GTCAGGAGTATTTAGCCTTGGAGGA	601	0.1085419439663502	No Hit
TCTAAGTACCCCGAGGAAAAGAAAT	596	0.10763893278526575	No Hit
CTATCGGTCAGTCAGGAGTATTTAG	595	0.10745833054904887	No Hit
CACACACACTGATTCAGGCTCTGGG	592	0.10691652384039818	No Hit
TCACGGTACTGGTTCACTATCGGTC	579	0.10456869476957863	No Hit
GCAGTGGTATCAACGCAGAGTACAT	575	0.10384628582471107	No Hit
TATTCAGACAGGATACCACGTGTCC	573	0.1034850813522773	No Hit
GGTATCAACGCAGAGTACATGGGGG	570	0.10294327464362664	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG	569	0.10276267240740976	No Hit
GTACTCTGCGTTGATACCACTGCTT	560	0.10113725228145774	No Hit
ATCATTAACTGAATCCATAGGTTAA	559	0.10095665004524086	No Hit
GTTGATACCACTGCTTATGTACTCT	559	0.10095665004524086	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.030111810844442E-4	0.0	0.0	0.0	0.0
2	9.030111810844442E-4	0.0	0.0	0.0	0.0
3	9.030111810844442E-4	0.0	0.0	0.0	0.0
4	9.030111810844442E-4	0.0	0.0	0.0	0.0
5	9.030111810844442E-4	0.0	0.0	0.0	0.0
6	0.001083613417301333	0.0	0.0	0.0	0.0
7	0.001083613417301333	0.0	0.0	0.0	0.0
8	0.001083613417301333	0.0	0.0	0.0	0.0
9	0.001083613417301333	0.0	0.0	0.0	0.0
10	0.001264215653518222	0.0	0.0	0.0	0.0
11	0.001264215653518222	0.0	0.0	0.0	0.0
12	0.001264215653518222	0.0	0.0	3.6120447243377765E-4	0.0
13	0.001264215653518222	0.0	0.0	5.418067086506665E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTCT	40	1.2815608E-5	19.002077	8
CTAAGAG	25	0.006026619	19.00036	3
GACCGAT	25	0.0060329107	18.99693	18
TGACCGA	25	0.0060329107	18.99693	17
ATAACCT	35	9.93726E-5	18.99693	19
CACCGTC	50	4.550784E-6	17.100325	7
CCGTCTG	45	3.5289384E-5	16.890736	9
TGCACCG	45	3.534411E-5	16.887686	5
AATAACC	40	2.7604934E-4	16.622313	18
CTCCTAT	35	0.0021662447	16.287495	10
ATAGATC	35	0.0021675522	16.286024	3
TAGATCC	35	0.0021688608	16.284554	4
TTTGGCA	35	0.0021701697	16.283083	18
GCACCGT	55	1.1264541E-5	15.544346	6
CGTCTGC	50	8.6896915E-5	15.201662	10
ACGCTTC	45	6.751631E-4	14.778059	7
CAATTTG	45	6.7608856E-4	14.775391	15
CATCTAC	40	0.005260133	14.255421	1
AGATCCT	40	0.005275922	14.248983	5
TTGGCAT	40	0.005279084	14.247697	19
>>END_MODULE