FastQCFastQC Report
Wed 25 May 2016
SRR1295182_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295182_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7329054
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT97520.1330594644274691No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN92400.12607356965851255No Hit
GTGGTATCAACGCAGAGTACATGGG88360.12056126206738277No Hit
GGTATCAACGCAGAGTACTTTTTTT77410.1056207254033058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49550.010.9528981
GTGGTAT22800.09.9632191
TGGTATC21950.09.698522
CGGCGTA800.0045235339.49880112
TACACCG3250.09.3583075
GTCTAGA7450.08.6753951
TAACGCA1107.116953E-48.6404244
GTACCGT1651.052651E-68.6358066
GTATCAA90200.08.6089891
TAGGACG2502.3464963E-108.3639294
GTCCTGG14550.08.29616451
CACACCG3450.08.2648095
ACACCGG2303.7071004E-98.2603376
TAGACAG10050.08.2278025
CTAGAAC8350.08.1954113
GTATTAA10950.08.1592521
ACCGTCC1752.4463934E-68.1421648
CCTACAC9750.08.0909253
CTAGACA7050.08.0889064
TAGAACT9300.08.0736864
Produced by FastQC (version 0.11.5)