FastQCFastQC Report
Wed 25 May 2016
SRR1295182_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295182_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7329054
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT241130.3290056261012676No Hit
GGTATCAACGCAGAGTACTTTTTTT196200.2677016706385299No Hit
TATCAACGCAGAGTACTTTTTTTTT139180.18990172537956468No Hit
GTGGTATCAACGCAGAGTACATGGG95520.13033059928334545No Hit
ACGCAGAGTACTTTTTTTTTTTTTT92230.12584161612126205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65700.011.83282851
AGCGTCA5550.010.9569543
GTGGTAT26900.010.8464291
TGGTATC26000.010.1959512
TAGACCG1401.0577642E-69.5016695
CCTATAC7450.09.0553443
GTCGTTA850.00744453458.9405512
CGACGTA850.0074482858.9400019
ACTCGCG850.0074482858.9400018
ATAACGC1951.6474587E-88.7708313
GTATCAA120050.08.6924231
GTCTTAG8100.08.6825521
CTATACT12250.08.6096754
TAGGACG2106.373739E-98.5967484
GTCGTAA1000.0028963558.5534871
AGGACCG2451.5643309E-108.5321115
CTAATAC11100.08.474523
ACCGATC1150.00111292588.2597858
GCGTCAG7500.08.2347794
GTCTAAG5200.08.2245061
Produced by FastQC (version 0.11.5)