FastQCFastQC Report
Wed 25 May 2016
SRR1295181_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295181_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5296607
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG70060.13227335915237812No Hit
GTATCAACGCAGAGTACTTTTTTTT62940.1188307911083454No Hit
GGTATCAACGCAGAGTACATGGGGG60310.11386534813702433No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN58900.11120326654403469No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGC500.001501602513.298569
GGTATCA37200.011.4216641
ATCGACC2200.011.2261658
GTCGTAC855.2981784E-511.1826791
GTGGTAT15950.010.9653361
ACCGGTC1201.5277637E-610.290658
TGGTATC16000.010.097922
GTCTTAG4050.09.6226271
TAGGACG1701.6554623E-89.5043754
CGCCTAA2357.2759576E-129.29651414
TCGACCG2600.09.1336269
CCGTATA950.0018181189.0037192
ATCGCTC1701.6901868E-78.9402938
GTATCAA69800.08.8652371
GTCGTAA1304.3446064E-58.7741021
TACATCG3300.08.6404255
TAGACCG1107.116124E-48.6404245
CACGGGT3200.08.6133414
GTATTAA9300.08.5854121
CTAGTCC4100.08.5770393
Produced by FastQC (version 0.11.5)