FastQCFastQC Report
Wed 25 May 2016
SRR1295181_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295181_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5296607
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT172900.32643539533894056No Hit
GGTATCAACGCAGAGTACTTTTTTT132990.25108527024942573No Hit
TATCAACGCAGAGTACTTTTTTTTT100230.18923435323783697No Hit
GTGGTATCAACGCAGAGTACATGGG76070.14362024594235517No Hit
GGTATCAACGCAGAGTACATGGGGG64850.12243687326622496No Hit
ACGCAGAGTACTTTTTTTTTTTTTT59200.11176966688296866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48950.012.0543041
GTGGTAT18750.011.7061461
TGGTATC18600.011.1345592
CGGTCGA600.00587782711.08358610
ACGGGTC2500.09.8804135
GTGTTAG5000.09.5017421
TAATGCG800.0045169259.5003975
GTCGCGC1201.7016444E-59.5003068
CGCCTAA1804.1909516E-99.50003714
ATACCGA1106.842753E-59.5000376
AGCGTCA4750.09.4002153
TCGACCG2151.07320375E-109.279379
CACGGGT2651.8189894E-128.9623844
AGTACCG1701.686567E-78.941555
CGGTCGT2151.0422809E-98.83741210
TACATCG3550.08.8313545
CGATATT1401.086271E-58.82146414
GTCCTAG4450.08.7544151
GTCTAGG4050.08.6806041
GTATCAA91250.08.673921
Produced by FastQC (version 0.11.5)