FastQCFastQC Report
Wed 25 May 2016
SRR1295180_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295180_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5724734
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75640.13212840980908458No Hit
GTGGTATCAACGCAGAGTACATGGG72710.12701026807533766No Hit
ATTCCATTCCATTCCATTCCATTCC61470.10737616804553714No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN61090.10671238174559726No Hit
GGTATCAACGCAGAGTACTTTTTTT59310.10360306697219468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40350.011.2609951
GTGGTAT18850.010.1362451
ACCGGCG1252.5793888E-69.87823212
GTATAGG4600.09.5058811
GTCCTAA4450.09.1854581
TGGTATC18650.09.1228882
ATCGCTA1151.1132042E-49.08580910
CGTCTTA850.0074475578.94004510
GTCTATA5900.08.8614151
TAGACAG7800.08.7745065
GTATCAA74600.08.6648791
GTATTAG8450.08.6621641
GACCGTG2201.6134436E-98.635127
GTCTAAC3200.08.6147051
AGGACCG1554.1783787E-68.5858075
GTCTAGG5650.08.580531
GTCGTAC1000.0028906218.5552941
ACTGTCG1451.6939186E-58.516098
CTAATAC8200.08.4622563
ACAATCG1252.7278703E-48.3650295
Produced by FastQC (version 0.11.5)