FastQCFastQC Report
Wed 25 May 2016
SRR1295180_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295180_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5724734
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT188850.32988432300959314No Hit
GGTATCAACGCAGAGTACTTTTTTT149470.2610951006631924No Hit
TATCAACGCAGAGTACTTTTTTTTT107940.18855024530397393No Hit
GTGGTATCAACGCAGAGTACATGGG77140.13474861888779463No Hit
ACGCAGAGTACTTTTTTTTTTTTTT68090.11894002411291076No Hit
ATTCCATTCCATTCCATTCCATTCC61870.10807489046652649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA4650.012.2587243
GGTATCA53300.011.3025721
GTGGTAT22350.010.7986991
TGGTATC21500.010.342
AGAACCG1752.3646862E-1010.3152015
CAGCGTC6300.09.1988272
TACCGTA1356.854878E-69.1482417
GCAGCGT6450.08.9863921
GGACCGA1701.6875128E-78.9412676
CTTAGAC4200.08.8219043
GTATCAA98950.08.738581
GTGCTAG6200.08.7357141
GTCTACG2304.0381565E-108.6757451
GTATTAG7250.08.6501011
GCGGTAT1107.1347156E-48.6381861
TAGGACT7400.08.6018144
TAGACAG8200.08.5739315
CGTGTAA1000.002906378.55016110
ACCGTCC1000.002906378.5501618
GTCTATA5050.08.46713351
Produced by FastQC (version 0.11.5)