FastQCFastQC Report
Wed 25 May 2016
SRR1295179_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295179_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473945
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA107642.271149605966937No Hit
GTACATAAGCAGTGGTATCAACGCA79921.6862716137948497No Hit
GCTTATGTACTCTGCGTTGATACCA46210.9750076485668169No Hit
GTATCAACGCAGAGTACTTTTTTTT40630.8572724683243836No Hit
CTTATGTACTCTGCGTTGATACCAC32340.682357657534102No Hit
GGTATCAACGCAGAGTACTTTTTTT28190.5947947546656258No Hit
TATCAACGCAGAGTACTTTTTTTTT23850.5032229478103999No Hit
GAGTACATAAGCAGTGGTATCAACG22120.46672082203631227No Hit
TATGTACTCTGCGTTGATACCACTG19160.40426631782168815No Hit
GTGGTATCAACGCAGAGTACATGGG16430.34666469738049777No Hit
GCGTTGATACCACTGCTTATGTACT16220.34223380350040616No Hit
CATAAGCAGTGGTATCAACGCAGAG14280.3013007838462269No Hit
GTACTGGTTCACTATCGGTCAGTCA14100.2975028748061484No Hit
ACGCAGAGTACATAAGCAGTGGTAT13330.28125626391247927No Hit
CTGCTTATGTACTCTGCGTTGATAC12490.263532688392113No Hit
ATACAGGGTGACAGCCCCGTACACA12480.263321693445442No Hit
GGATACCACGTGTCCCGCCCTACTC11200.2363143402715505No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9950.2099399719376721No Hit
GTATCAACGCAGAGTACATAAGCAG9910.209095992150988No Hit
ACTCTGCGTTGATACCACTGCTTAT9830.20740803257761975No Hit
GGTATCAACGCAGAGTACATGGGGG9710.20487609321756745No Hit
TATCAACGCAGAGTACATAAGCAGT9650.2036101235375413No Hit
GGTATCAACGCAGAGTACATAAGCA9600.20255514880418612No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9520.20086718923081792No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA8740.1844095833904778No Hit
ACCCTGTATCGCGCGCCTTTCCAGA7880.16626401797676946No Hit
GTGGTATCAACGCAGAGTACATAAG7370.15550327569654707No Hit
CCCATGTACTCTGCGTTGATACCAC7070.14917342729641625No Hit
GTATTTAGCCTTGGAGGATGGTCCC6990.14748546772304802No Hit
GTTCACTATCGGTCAGTCAGGAGTA6800.14347656373629852No Hit
CCCATATTCAGACAGGATACCACGT6480.13672472544282566No Hit
ACATAAGCAGTGGTATCAACGCAGA6470.13651373049615462No Hit
TCTAAGTACCCCGAGGAAAAGAAAT6380.13461477597611538No Hit
AAGCAGTGGTATCAACGCAGAGTAC5950.1255419932692612No Hit
GGTTAATGAGGCGAACCGGGGGAAC5720.12068910949582758No Hit
GCCTTGGAGGATGGTCCCCCCATAT5630.11879015497578832No Hit
GTTAATGATAGTGTGTCGAAACACA5560.11731319034909114No Hit
GTATCAACGCAGAGTACATGGGGGT5550.11710219540242012No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN5330.11246030657565752No Hit
TATTCAGACAGGATACCACGTGTCC5270.11119433689563134No Hit
CCACTGCTTATGTACTCTGCGTTGA5220.11013936216227621No Hit
GTCAGGAGTATTTAGCCTTGGAGGA5160.10887339248225004No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG5130.10824040764223697No Hit
GAGTACTTTTTTTTTTTTTTTTTTT5110.10781841774889492No Hit
GATACCACTGCTTATGTACTCTGCG5050.10655244806886874No Hit
CTATCGGTCAGTCAGGAGTATTTAG5040.10634145312219771No Hit
CATGTACTCTGCGTTGATACCACTG5000.10549747333551361No Hit
ATACCACTGCTTATGTACTCTGCGT4770.10064458956207999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC504.516469E-617.1127824
CAACTGA402.7603508E-416.6215726
ATGTATG350.002151819216.3030721
TGTGCCG350.002170179716.28235613
AGCTAAC350.002170179716.2823567
ACAGTTG350.002174790516.27718419
CTGGGTA551.1294589E-515.5389589
GTACAAA755.740185E-815.2162011
CCTTAGG704.363992E-714.9444831
TCTTAGA456.711849E-414.7888262
GCTGTGC456.760565E-414.77473111
TTAGGAC653.334153E-614.626313
AGCTATA400.0052347614.265191
GTAAGAC602.542806E-514.2606533
GCCCAAT400.005279120514.2470627
GAGTCTA400.005279120514.2470627
GACCGTT602.5657826E-514.2470627
TGTTACG602.568347E-514.24555316
ACTAAGC400.0052902614.24253719
CGTAAGA400.0052902614.24253718
Produced by FastQC (version 0.11.5)