FastQCFastQC Report
Wed 25 May 2016
SRR1295179_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295179_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473945
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT113482.394370654822817No Hit
GTACATAAGCAGTGGTATCAACGCA83861.7694036227832344No Hit
GGTATCAACGCAGAGTACTTTTTTT78841.6634841595543786No Hit
TATCAACGCAGAGTACTTTTTTTTT68271.440462500923103No Hit
GCTTATGTACTCTGCGTTGATACCA47120.9942081887138804No Hit
CTTATGTACTCTGCGTTGATACCAC37620.7937629893764043No Hit
ACGCAGAGTACTTTTTTTTTTTTTT37610.7935519944297333No Hit
GTACTTTTTTTTTTTTTTTTTTTTT32370.6829906423741152No Hit
GAGTACATAAGCAGTGGTATCAACG24380.5144056799839644No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20120.42452183270210675No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA18410.3884416968213611No Hit
GCGTTGATACCACTGCTTATGTACT17870.37704796970112564No Hit
CATAAGCAGTGGTATCAACGCAGAG17640.372195085927692No Hit
TATGTACTCTGCGTTGATACCACTG17400.3671312072075874No Hit
GTGGTATCAACGCAGAGTACATGGG17240.36375528806085095No Hit
GTACTGGTTCACTATCGGTCAGTCA14820.3126945109664624No Hit
GCAGAGTACTTTTTTTTTTTTTTTT14650.3091075968730549No Hit
ATACAGGGTGACAGCCCCGTACACA14610.3082636170863708No Hit
CTGCTTATGTACTCTGCGTTGATAC14570.3074196372996867No Hit
ACGCAGAGTACATAAGCAGTGGTAT13990.2951819303927671No Hit
ACTCTGCGTTGATACCACTGCTTAT11750.247919062338457No Hit
GGATACCACGTGTCCCGCCCTACTC11730.24749707244511493No Hit
GTATCAACGCAGAGTACATAAGCAG11490.24243319372501027No Hit
ATCAACGCAGAGTACTTTTTTTTTT10840.22871852219139352No Hit
GGTATCAACGCAGAGTACATGGGGG10640.22449862325797298No Hit
GTGGTATCAACGCAGAGTACTTTTT10550.22259966873793371No Hit
GGTATCAACGCAGAGTACATAAGCA10270.21669181023114495No Hit
TATCAACGCAGAGTACATAAGCAGT9970.21036196183101416No Hit
ACCCTGTATCGCGCGCCTTTCCAGA8770.18504256823049087No Hit
CTTATACACATCTCCGAGCCCACGA8580.18103366424374137No Hit
CCCATGTACTCTGCGTTGATACCAC8300.17512580573695258No Hit
ACATAAGCAGTGGTATCAACGCAGA7680.16204411904334892No Hit
CCCATATTCAGACAGGATACCACGT7660.16162212915000687No Hit
GTGGTATCAACGCAGAGTACATAAG7510.15845720494994145No Hit
AAGCAGTGGTATCAACGCAGAGTAC6870.14495352836299571No Hit
GCCTTGGAGGATGGTCCCCCCATAT6800.14347656373629852No Hit
GTTCACTATCGGTCAGTCAGGAGTA6690.14115561932291723No Hit
GTATTTAGCCTTGGAGGATGGTCCC6370.13440378102944434No Hit
TCTAAGTACCCCGAGGAAAAGAAAT6090.12849592252265557No Hit
GTATCAACGCAGAGTACATGGGGGT5940.12533099832259018No Hit
GATACCACTGCTTATGTACTCTGCG5760.12153308928251169No Hit
CTATCGGTCAGTCAGGAGTATTTAG5690.1200561246558145No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG5620.1185791600291173No Hit
CCCCAGTAGCGGCGAGCGAACGGGG5570.11752418529576217No Hit
GGTTAATGAGGCGAACCGGGGGAAC5510.11625821561573599No Hit
GTTAATGATAGTGTGTCGAAACACA5460.11520324088238086No Hit
CATGTACTCTGCGTTGATACCACTG5250.11077234700228929No Hit
CATCTAAGTACCCCGAGGAAAAGAA5240.11056135205561826No Hit
CACACACACTGATTCAGGCTCTGGG5220.11013936216227621No Hit
CCACTGCTTATGTACTCTGCGTTGA5210.10992836721560519No Hit
GTCAGGAGTATTTAGCCTTGGAGGA5200.10971737226893416No Hit
CAACGCAGAGTACTTTTTTTTTTTT5150.10866239753557902No Hit
GCCCAGAGCCTGAATCAGTGTGTGT4880.10296553397546129No Hit
TATTCAGACAGGATACCACGTGTCC4850.10233254913544822No Hit
ATACCACTGCTTATGTACTCTGCGT4750.10022259966873794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC552.8068825E-819.0117231
GTACAAA401.2753288E-519.011721
ACTAGAG250.006019261719.0036953
AGGACTG453.5246325E-516.8921725
TATACTT402.7519526E-416.6282315
GCTTGTC402.7541697E-416.6264762
GACCGTT753.263267E-916.4663937
CGTTATT753.263267E-916.46639310
TACGGCC753.268724E-916.46465519
ACACGTA350.002164393216.2888815
AGTACAC350.0021659216.2871632
CGTAATA350.00216744816.2854428
ACGTAAT350.00216744816.2854427
GGACAGT350.00216744816.2854426
GGTTTAT651.8917308E-716.0868421
CGAATGG551.1263126E-515.54355719
CCGAATG551.1263126E-515.54355718
TGTTACG807.692506E-915.43398616
GTAGGAC508.6791726E-515.2029553
TAGGACC953.0195224E-1015.0029174
Produced by FastQC (version 0.11.5)