##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295179_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	473945
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.8322168184072	31.0	30.0	33.0	28.0	34.0
2	30.79982698414373	31.0	30.0	33.0	27.0	34.0
3	30.975189104220952	31.0	31.0	34.0	27.0	34.0
4	34.58668621886506	37.0	35.0	37.0	32.0	37.0
5	34.86880123221049	37.0	35.0	37.0	32.0	37.0
6	34.54573209971621	37.0	35.0	37.0	31.0	37.0
7	34.450358163921976	37.0	35.0	37.0	30.0	37.0
8	34.45719440019411	37.0	35.0	37.0	30.0	37.0
9	35.93450505860385	39.0	37.0	39.0	30.0	39.0
10	35.990239373767	39.0	37.0	39.0	30.0	39.0
11	36.00771186530083	39.0	37.0	39.0	30.0	39.0
12	35.95843610545528	39.0	37.0	39.0	30.0	39.0
13	35.75641477386617	39.0	35.0	39.0	30.0	39.0
14	36.810674234352085	40.0	37.0	41.0	29.0	41.0
15	36.79735834326768	40.0	36.0	41.0	29.0	41.0
16	36.741974279716004	40.0	36.0	41.0	29.0	41.0
17	36.88333034423825	40.0	37.0	41.0	30.0	41.0
18	36.9305911023431	40.0	37.0	41.0	30.0	41.0
19	37.052518752175885	40.0	37.0	41.0	30.0	41.0
20	37.04811950753779	40.0	37.0	41.0	30.0	41.0
21	37.02431505765437	40.0	37.0	41.0	30.0	41.0
22	36.99546571859604	40.0	37.0	41.0	30.0	41.0
23	36.92508835413392	40.0	36.0	41.0	29.0	41.0
24	36.94290476743082	40.0	36.0	41.0	29.0	41.0
25	35.637460042831975	39.0	34.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.04918433239081921
1101	2	0.21694578921920993
1101	3	0.3002001462838102
1101	4	-0.15391026445293932
1101	5	0.057684464764356846
1101	6	-0.049225066924400096
1101	7	-0.053600630862298715
1101	8	0.10320781149301439
1101	9	0.1135737801419694
1101	10	0.28715140872049716
1101	11	0.20969570955033845
1101	12	0.40002268422480114
1101	13	0.3054274099949694
1101	14	0.6173238836167272
1101	15	0.524476531663332
1101	16	0.4862523438591424
1101	17	0.45606044123084644
1101	18	0.39033841398831726
1101	19	0.5203452604388588
1101	20	0.3343373177496147
1101	21	0.1300424274450549
1101	22	0.24659551573495975
1101	23	0.3828385019572238
1101	24	0.09640868582156514
1101	25	0.46765054364307446
1102	1	-0.018641710153676883
1102	2	0.006464163028553571
1102	3	-0.014395476689198716
1102	4	-0.23242988957104416
1102	5	-0.09621374509799807
1102	6	-0.23861320247433326
1102	7	-0.2611072066175524
1102	8	0.031040493635046573
1102	9	0.12539098691436124
1102	10	-0.0010007402107845564
1102	11	0.10099145254684316
1102	12	0.08875962847771746
1102	13	0.25882892688763803
1102	14	0.11465527614462445
1102	15	0.34596073708226527
1102	16	-0.05968856372277287
1102	17	0.12405502740516283
1102	18	0.2922000549903814
1102	19	0.19222145748167918
1102	20	0.07714229234039038
1102	21	0.15679234445008206
1102	22	0.29147025717370667
1102	23	0.3957346433295754
1102	24	0.3654528391451919
1102	25	0.5412351602063126
1103	1	-0.31618285377227906
1103	2	-0.23881673394052783
1103	3	-0.11713569685742087
1103	4	-0.15516094313625928
1103	5	-0.25845837457202236
1103	6	-0.04628216927935114
1103	7	0.007777693101509442
1103	8	0.1843519289723048
1103	9	-0.03019300208571707
1103	10	0.23838127796964415
1103	11	0.22068140068763142
1103	12	0.27559494366457216
1103	13	0.2965966044269166
1103	14	0.37670978348215556
1103	15	0.38863212285195914
1103	16	0.15478024012041658
1103	17	-0.04844686108480545
1103	18	0.029551415976300177
1103	19	-0.041399605335016076
1103	20	0.17219062919607353
1103	21	0.3532674460890419
1103	22	0.17625751559552327
1103	23	0.15562905764305413
1103	24	0.2270074271148701
1103	25	-0.03484764477420299
1104	1	-0.09975358073923246
1104	2	0.009065362998171622
1104	3	-0.02538886926153694
1104	4	-0.131666617755144
1104	5	-0.2965251296199085
1104	6	-0.12702741983591892
1104	7	-0.11439573095870514
1104	8	-0.04148931573686809
1104	9	0.03108324786978045
1104	10	-0.12094074172973279
1104	11	-0.076440764359063
1104	12	-0.11973509317490283
1104	13	0.19106481964984567
1104	14	-0.031087869598515283
1104	15	0.012513482582377833
1104	16	0.0964214081766741
1104	17	-0.051611411419166586
1104	18	0.1338496334820647
1104	19	-0.052709050606722485
1104	20	-0.22866009240936336
1104	21	-0.2214639022272138
1104	22	-0.06565361310806139
1104	23	-0.053874227331924374
1104	24	-0.034980061287711806
1104	25	0.35702463389052497
1105	1	-0.28542734664222635
1105	2	-0.39782796653125985
1105	3	-0.44907517976733047
1105	4	-0.30110144908109504
1105	5	-0.31054374392505935
1105	6	-0.1514426734775327
1105	7	-0.05346716573214394
1105	8	-0.2535319690402673
1105	9	-0.08027180904401376
1105	10	0.01449466018131318
1105	11	-0.2535846965173221
1105	12	-0.28481817946142485
1105	13	-0.09781662682472358
1105	14	-0.2283883405379541
1105	15	-0.17793376517145987
1105	16	-0.33196189269946075
1105	17	-0.3968484729886086
1105	18	-0.29046721490355054
1105	19	-0.16838277516296785
1105	20	-0.3203258287742443
1105	21	-0.6208011138853422
1105	22	-0.2670804893200369
1105	23	-0.15872337372834977
1105	24	-0.25186648591052574
1105	25	-0.12721605378855827
1106	1	-0.39868034841600064
1106	2	-0.47479105835824953
1106	3	-0.21753353015619936
1106	4	-0.15917383276235597
1106	5	0.011059646714869587
1106	6	-0.12617063772913895
1106	7	-0.43108945390243036
1106	8	-0.46050085974841437
1106	9	-0.5137850782485458
1106	10	-0.45761101590000663
1106	11	-0.13817416984246478
1106	12	-0.49807203401184097
1106	13	-0.6594041558191321
1106	14	-0.5465550413156919
1106	15	-0.6102318276629362
1106	16	-0.824935013179747
1106	17	-0.5535504446082058
1106	18	-0.4960204963880628
1106	19	-0.6019514748491446
1106	20	-0.7216254603747316
1106	21	-0.753164840178151
1106	22	-0.4952621989666426
1106	23	-0.590219826177524
1106	24	-0.21589636720401728
1106	25	-0.29624917563328523
1107	1	-0.1335099309339398
1107	2	-0.008311932461314342
1107	3	0.14581040330759976
1107	4	0.10427202503281308
1107	5	0.03962699831303951
1107	6	0.07556207590425856
1107	7	0.2679443034361242
1107	8	0.2737946392731132
1107	9	0.30235565886821547
1107	10	0.16529341984689694
1107	11	0.27782795018362805
1107	12	0.1785016776646131
1107	13	0.36184502878622027
1107	14	0.27782118177628945
1107	15	0.24114618929727527
1107	16	0.2776742497034874
1107	17	0.07658246471991248
1107	18	0.09510800980893919
1107	19	0.03896502873858765
1107	20	0.1487757137935617
1107	21	0.09407504892817542
1107	22	0.135702412905502
1107	23	-0.29903424235495635
1107	24	0.0972641024633063
1107	25	-0.21557810583550463
1108	1	0.44237578747708994
1108	2	0.4847539256432576
1108	3	0.20191522760777758
1108	4	0.37834028355610627
1108	5	0.25714825014042475
1108	6	0.510716478777546
1108	7	0.3527603014147189
1108	8	0.37290305517723965
1108	9	0.28618080731516216
1108	10	0.34684268257542783
1108	11	0.6859659110910954
1108	12	0.387762240409387
1108	13	0.3949671974652702
1108	14	0.4760957680199027
1108	15	0.37891037071839406
1108	16	0.47329574157274834
1108	17	0.44051481983749596
1108	18	0.24678018119521994
1108	19	0.47749987090654855
1108	20	0.4425237961658297
1108	21	0.49219442012675785
1108	22	0.29973944793717777
1108	23	0.21590430331616517
1108	24	0.24709535122248383
1108	25	0.19496929792214246
1109	1	0.10714084026098192
1109	2	-0.04009482470105752
1109	3	0.17283845616981708
1109	4	0.017246042492530478
1109	5	0.060833996001179
1109	6	0.13462836071154527
1109	7	-0.0964254560614819
1109	8	-0.02450769674013742
1109	9	-0.16597186606190917
1109	10	-0.19803772814661613
1109	11	0.04716303178881276
1109	12	0.06993054485345596
1109	13	0.045330760817549276
1109	14	0.0784112235277803
1109	15	0.1668837414866431
1109	16	0.07496902904167513
1109	17	-0.0066548286792098565
1109	18	0.13700097416489143
1109	19	-0.07758719513222445
1109	20	0.06926154934099316
1109	21	0.17085983896302537
1109	22	0.11213179767483439
1109	23	0.19104891557610415
1109	24	0.03477833592661028
1109	25	0.1479566238149559
1110	1	0.07647551098281724
1110	2	0.06078985134238124
1110	3	0.25719552599891315
1110	4	0.2244498792064249
1110	5	0.014594111076796423
1110	6	0.22132532288301832
1110	7	0.04833824867668568
1110	8	0.14762516915226342
1110	9	0.09616058055274834
1110	10	0.25728822804853024
1110	11	0.2311421066040893
1110	12	0.2369707987422558
1110	13	0.3233941491622758
1110	14	0.3512465409049099
1110	15	-0.01207851384060632
1110	16	0.3440485853813158
1110	17	0.06366611230905761
1110	18	0.14659275890124945
1110	19	0.22843234321072714
1110	20	0.24496956431888606
1110	21	0.20519707372953633
1110	22	0.22002002467825577
1110	23	0.23850631856974758
1110	24	0.09407711002297958
1110	25	0.34165971711716026
1111	1	0.018979631192969748
1111	2	-0.03631648716957159
1111	3	0.01792661737888679
1111	4	-0.08728796254525406
1111	5	0.23967530582486063
1111	6	-0.1777142085579726
1111	7	-0.02967482633855667
1111	8	-0.09251777230688418
1111	9	0.15014904073910174
1111	10	-0.11995208582924022
1111	11	0.05849804438327766
1111	12	-0.1441752485974206
1111	13	-0.1612183575703554
1111	14	-0.18733184952007775
1111	15	0.08753865332543143
1111	16	-0.10513172662582804
1111	17	-0.08599991684042152
1111	18	0.06647200691048738
1111	19	-0.07003052006925259
1111	20	0.0037703654618965743
1111	21	0.1456709220864525
1111	22	0.026489480294479506
1111	23	-0.11121808694279167
1111	24	-0.1113173819575266
1111	25	-0.2852927464621189
1112	1	0.07204775145092057
1112	2	0.074314276363733
1112	3	-0.07041822899320493
1112	4	-0.057430067212393965
1112	5	0.07478737048967332
1112	6	0.015028445184704253
1112	7	0.08819430759731262
1112	8	0.06525421749512361
1112	9	0.0749817012457541
1112	10	0.001637233825157125
1112	11	0.046514179981059556
1112	12	-0.003001010462689635
1112	13	-0.09036955534467239
1112	14	-0.06827978528374956
1112	15	-0.05703287493481923
1112	16	0.11428271522388656
1112	17	-0.09214870590543
1112	18	0.033603990101553904
1112	19	0.13411141932432002
1112	20	-0.1758651757019365
1112	21	0.061438021014929234
1112	22	-0.1294384849123702
1112	23	0.016839856747893123
1112	24	0.14356813548648972
1112	25	0.254959020281774
1113	1	0.17660018245663522
1113	2	-0.0025735828512978287
1113	3	0.15838495924913332
1113	4	0.12695341718067965
1113	5	0.11657200716143734
1113	6	0.2756079564836682
1113	7	0.13788269237235085
1113	8	0.009509393156577062
1113	9	0.006969934282778922
1113	10	0.03966911624854674
1113	11	-0.0487958946089293
1113	12	0.1698335253410832
1113	13	-0.1351104670517529
1113	14	0.021220932710285467
1113	15	-0.12081225600652346
1113	16	-0.09105485824695592
1113	17	-0.02613104383353715
1113	18	-0.02279197052796178
1113	19	0.08297105875760025
1113	20	0.10877440291986318
1113	21	0.09445847948464348
1113	22	0.16946068779571277
1113	23	0.16181651467460512
1113	24	0.19652673600856474
1113	25	0.16324472958430647
1114	1	0.10371811096775119
1114	2	0.24205067833642957
1114	3	0.315061007658926
1114	4	0.5928779879659345
1114	5	0.34765278396977095
1114	6	0.4375443482652628
1114	7	0.43711432761017477
1114	8	0.5055915772263759
1114	9	0.21650553799795347
1114	10	0.37760607093472487
1114	11	0.3554427769362434
1114	12	0.559371943579194
1114	13	0.3867443035646261
1114	14	0.3940016813733891
1114	15	0.5496071079112923
1114	16	0.2858773117502267
1114	17	0.2615300876046831
1114	18	0.3615705400265057
1114	19	0.3662978248707418
1114	20	0.4053913463479546
1114	21	0.16870840912157092
1114	22	0.3033897618176695
1114	23	0.368008465138999
1114	24	0.1476750613674156
1114	25	0.14878933977287545
1115	1	0.15260249230649592
1115	2	0.01595196298822188
1115	3	0.0050136783823901965
1115	4	-0.2054587260300096
1115	5	0.32348780763440743
1115	6	0.06460317179467268
1115	7	0.015369905703479958
1115	8	-0.15528739505219136
1115	9	-0.15431258235363998
1115	10	-0.08312415218900782
1115	11	-0.017227946525551374
1115	12	0.028918934789473383
1115	13	-0.30690005072881377
1115	14	-0.2926244173419619
1115	15	-0.4900142578763962
1115	16	-0.1955425665459174
1115	17	-0.5742914134095187
1115	18	-0.3961847000005392
1115	19	-0.3559334124517761
1115	20	-0.4941624061875416
1115	21	-0.22526048878241767
1115	22	-0.26185702655285326
1115	23	-0.0712748343877081
1115	24	-0.24680769725327423
1115	25	-0.47860755001752153
1116	1	0.3222276453657038
1116	2	0.1323006614912039
1116	3	0.29375247049835806
1116	4	0.44501328882167
1116	5	0.5428107921902381
1116	6	0.4439846150936404
1116	7	0.14335110410771534
1116	8	0.21715009754171888
1116	9	0.22681077349421486
1116	10	0.025933003082158734
1116	11	0.13401430176610774
1116	12	-0.07873600062941932
1116	13	0.2231232537701331
1116	14	0.1513104880575682
1116	15	0.22951412226343137
1116	16	0.11312749704771363
1116	17	0.10289387710673736
1116	18	0.16909159655908468
1116	19	-0.05116191503896772
1116	20	-0.012596659851084269
1116	21	0.16966387034386088
1116	22	0.24768596827276212
1116	23	0.18364083492540573
1116	24	0.2720965963044151
1116	25	-1.1332388254450976
1201	1	-0.21689159276775527
1201	2	-0.25893686996756315
1201	3	-0.2844065749945699
1201	4	-0.4577617715875206
1201	5	-0.6068083581513122
1201	6	-0.6945209157965664
1201	7	-0.40292860927260676
1201	8	-0.566731308132141
1201	9	-0.7505790476611267
1201	10	-0.5221878320279174
1201	11	-0.601354131608197
1201	12	-0.5974507270949161
1201	13	-0.6769310936015387
1201	14	-0.5742336127442584
1201	15	-0.632316464437352
1201	16	-0.4796100218693553
1201	17	-0.7167781992136923
1201	18	-0.5883736420258785
1201	19	-0.6825128234761877
1201	20	-0.552204188216237
1201	21	-0.3017853650304261
1201	22	-0.4417798388693228
1201	23	-0.7346106847107237
1201	24	-0.6232738437420764
1201	25	-0.32180748289779615
1202	1	0.02634254535689351
1202	2	0.048472537020732176
1202	3	-0.08146527149178695
1202	4	-0.3007454022185456
1202	5	-0.05174853868947338
1202	6	-0.32533765660769376
1202	7	-0.12474435669194861
1202	8	-0.1660164979563561
1202	9	-0.07532530084646538
1202	10	-0.12954325033224023
1202	11	-0.11815972915494655
1202	12	-0.22568896835954178
1202	13	0.01617347168157579
1202	14	-0.13370106836556062
1202	15	-0.16001697840962237
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2210	21	0.2514643729680657
2210	22	0.3312753745010326
2210	23	0.407673600714638
2210	24	0.28414861300469596
2210	25	0.03586594371419949
2211	1	0.1902137618596882
2211	2	0.11653099677959489
2211	3	0.10820268538265054
2211	4	0.3337772950571889
2211	5	0.38081215298053195
2211	6	0.271620715654322
2211	7	0.18145753857877622
2211	8	0.41324600741950945
2211	9	0.4355413628542095
2211	10	0.32042783121778484
2211	11	0.33762470233798325
2211	12	0.14494175044931268
2211	13	0.3321371975643288
2211	14	0.15684408149132878
2211	15	0.3476315808583692
2211	16	0.034714109627273615
2211	17	0.243520357396811
2211	18	0.2959594534866241
2211	19	0.28244702139145517
2211	20	0.3545023591741412
2211	21	0.11878064854365533
2211	22	0.14297401657220377
2211	23	0.20567616379741338
2211	24	0.09394072552781552
2211	25	-0.14046910044531558
2212	1	0.11203504368879535
2212	2	0.15347279365095545
2212	3	0.010653980907708416
2212	4	0.09057169741997484
2212	5	0.008929844980045232
2212	6	0.14737888431015733
2212	7	0.022458740468259464
2212	8	0.08771563874690003
2212	9	-0.04295506408674754
2212	10	-0.09718217893771453
2212	11	-0.2510868535032884
2212	12	-0.21564372811699428
2212	13	-0.27334025070317836
2212	14	-0.2773315061767292
2212	15	-0.3414563806811657
2212	16	-0.11233101247165678
2212	17	-0.12675477280788527
2212	18	-0.05072044328132108
2212	19	-0.09369780163265062
2212	20	-0.10078548869832815
2212	21	-0.09533270174657105
2212	22	-0.1409793612962602
2212	23	-0.15227726997289182
2212	24	0.035630451701983645
2212	25	-0.2390100625159164
2213	1	0.17526259082866247
2213	2	0.0737516345809972
2213	3	0.08834701171572235
2213	4	0.18862994392073062
2213	5	0.1969689242189716
2213	6	0.15548987130205916
2213	7	0.23778369880692907
2213	8	0.13494249000528669
2213	9	0.15241976575801885
2213	10	0.16564432071739787
2213	11	0.12603970536278553
2213	12	-0.0281418205471482
2213	13	0.05878139426297224
2213	14	0.1506432345463793
2213	15	-0.023922303853410654
2213	16	0.01701900980876303
2213	17	0.05477262599934818
2213	18	0.07089247569732038
2213	19	0.14702225257286017
2213	20	0.10041887460063492
2213	21	0.11603671975616692
2213	22	-0.14248100028027721
2213	23	0.12888936922477257
2213	24	0.026098706687612605
2213	25	0.11195074226490931
2214	1	0.44156559535240447
2214	2	0.3782851487873877
2214	3	0.45507336265927023
2214	4	0.5575879886523225
2214	5	0.5408753798926185
2214	6	0.5999590644434463
2214	7	0.4792133048907061
2214	8	0.41033177902471607
2214	9	0.46631403549262984
2214	10	0.6031109099765288
2214	11	0.45167587460938563
2214	12	0.5399169363721086
2214	13	0.5946034565607121
2214	14	0.3552879741868935
2214	15	0.5434639271850941
2214	16	0.5356528625773223
2214	17	0.666713696637558
2214	18	0.556627167111408
2214	19	0.5534980445151945
2214	20	0.3832045112872109
2214	21	0.522309891721612
2214	22	0.4142209787852025
2214	23	0.4394066393445044
2214	24	0.43656654328106725
2214	25	0.4620421368076748
2215	1	0.16286768303676524
2215	2	0.031236930204382674
2215	3	0.2889721610348275
2215	4	0.16118935870360218
2215	5	0.14185737573718882
2215	6	-0.03193262497207172
2215	7	0.1466592264010984
2215	8	-0.06389083005524299
2215	9	-0.052650826626418734
2215	10	-0.025705979058479045
2215	11	-0.17894385531480594
2215	12	-0.13854410043656173
2215	13	-0.1063001197474236
2215	14	-0.10795185754795966
2215	15	-0.036047015169806684
2215	16	-0.06500979597970513
2215	17	0.08632019764407772
2215	18	-0.1963944103878461
2215	19	0.03388103367389306
2215	20	-0.05060104599004234
2215	21	0.08533386761905604
2215	22	-0.11879472398589286
2215	23	0.05375768399513703
2215	24	-0.07052355295129331
2215	25	0.012913779387062618
2216	1	0.19941946843381686
2216	2	0.047254298245931636
2216	3	-0.10661320048586731
2216	4	0.16749337258131902
2216	5	0.28561658487474517
2216	6	-0.011641172096730656
2216	7	0.1285622914110789
2216	8	0.0856368260951541
2216	9	-0.15729536697942592
2216	10	-0.2607649697892569
2216	11	-0.026095684602218228
2216	12	-0.1270986446561011
2216	13	-0.007147099150309089
2216	14	0.05042702073990313
2216	15	-0.17550601426064816
2216	16	-0.5203670321411735
2216	17	-0.2267957042505202
2216	18	-0.2378864735481585
2216	19	-0.23605511630572806
2216	20	-0.10890729618598272
2216	21	-0.11486625151191276
2216	22	-0.09004915220948106
2216	23	-0.1815390461732207
2216	24	-0.148750277999099
2216	25	-0.49931020773843215
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	14.0
9	71.0
10	144.0
11	276.0
12	423.0
13	634.0
14	1051.0
15	1680.0
16	2424.0
17	2853.0
18	3279.0
19	3273.0
20	3209.0
21	3248.0
22	3148.0
23	3098.0
24	3138.0
25	3154.0
26	3319.0
27	3794.0
28	4401.0
29	5293.0
30	6627.0
31	8798.0
32	11562.0
33	15643.0
34	22153.0
35	32464.0
36	48767.0
37	84093.0
38	191914.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.36460597568027	19.299193422446574	12.756249815343967	22.579950786529185
2	14.83110838223162	22.331588397760914	38.80361140474699	24.033691815260486
3	18.512086998537786	28.92650741973123	30.34905335918757	22.21235222254341
4	11.554927259492136	20.042409984280877	35.61510301828271	32.78755973794428
5	11.8030863507163	39.794637186031444	32.299485326856484	16.10279113639577
6	27.98964417136491	37.1115509267074	19.115661186320516	15.78314371560717
7	23.790282592687152	37.092162816263055	20.152215608535386	18.965338982514407
8	23.776266565950472	34.67893547481849	21.5306189193124	20.014179039918638
9	26.480691404288358	17.62207395966527	20.16272307958492	35.73451155646145
10	17.040975650926278	28.368991855509133	28.928134362999536	25.661898130565053
11	29.63201316650138	27.218998586289118	21.02968792859705	22.119300318612453
12	24.191462109974236	28.012221704075234	27.142438137118575	20.653878048831952
13	29.043558267967445	22.850100554792338	27.64634257002433	20.459998607215887
14	24.14926727366722	25.614508930926434	26.813880126182966	23.422343669223384
15	26.83180537826119	26.9016447056093	26.188692780807898	20.077857135321608
16	21.309223644093723	28.508582219455842	27.306544008271	22.87565012817943
17	19.283120544031664	29.779108458190116	27.484528730248154	23.45324226753006
18	18.51432984274414	24.661719496730605	34.82627584446165	21.997674816063604
19	20.862512422432328	28.185997835173577	30.900048740244042	20.051441002150057
20	21.01427410931879	25.395676622321627	33.51155749206115	20.07849177629844
21	23.195763176773433	26.725745875005273	29.849770013081823	20.228720935139467
22	21.123892797008917	26.006346768169948	30.658603795401124	22.21115663942001
23	23.379393724561815	24.15649139242483	30.968702987490055	21.4954118955233
24	20.98372347204463	25.559255086151232	30.176775669186007	23.280245772618127
25	21.49487157304817	27.322351332589196	31.455688323307175	19.727088771055456
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2445.0
1	2445.0
2	1989.5
3	1534.0
4	1534.0
5	1534.0
6	3095.0
7	4656.0
8	4656.0
9	4656.0
10	4425.5
11	4195.0
12	4195.0
13	4195.0
14	3792.5
15	3390.0
16	3390.0
17	3390.0
18	5585.5
19	7781.0
20	7781.0
21	7781.0
22	10922.0
23	14063.0
24	14063.0
25	14063.0
26	18942.0
27	23821.0
28	23821.0
29	23821.0
30	27890.0
31	31959.0
32	31959.0
33	31959.0
34	33563.5
35	35168.0
36	35168.0
37	35168.0
38	35108.5
39	35049.0
40	35049.0
41	35049.0
42	54849.0
43	74649.0
44	74649.0
45	74649.0
46	68968.5
47	63288.0
48	63288.0
49	63288.0
50	60205.5
51	57123.0
52	57123.0
53	57123.0
54	51153.5
55	45184.0
56	45184.0
57	45184.0
58	37821.0
59	30458.0
60	30458.0
61	30458.0
62	26436.5
63	22415.0
64	22415.0
65	22415.0
66	15583.0
67	8751.0
68	8751.0
69	8751.0
70	6844.0
71	4937.0
72	4937.0
73	4937.0
74	3596.5
75	2256.0
76	2256.0
77	2256.0
78	1430.5
79	605.0
80	605.0
81	605.0
82	383.5
83	162.0
84	162.0
85	162.0
86	100.5
87	39.0
88	39.0
89	39.0
90	26.5
91	14.0
92	14.0
93	14.0
94	8.5
95	3.0
96	3.0
97	3.0
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.019200540147063478
2	4.2198989334205447E-4
3	0.0012659696800261635
4	0.0
5	0.010971737226893416
6	0.0018989545200392452
7	0.002531939360052327
8	0.0012659696800261635
9	0.0023209444133813
10	0.0010549747333551361
11	0.003164924200065408
12	0.007173828186814926
13	0.015613626053656016
14	0.006329848400130816
15	0.0
16	0.0
17	4.2198989334205447E-4
18	8.439797866841089E-4
19	8.439797866841089E-4
20	0.0021099494667102723
21	0.0010549747333551361
22	6.329848400130817E-4
23	4.2198989334205447E-4
24	0.0023209444133813
25	0.0037979090400784904
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	473945.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.48592797589326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.47596485694291	16.216711448273262
2	7.550599406590767	3.2446327004966324
3	3.272592461983427	2.1094405779788135
4	1.9876800852628853	1.7082860460430294
5	1.5028777850409631	1.6145361922980064
6	1.0911763840877087	1.4066962318502487
7	0.8973219950018044	1.3495857029055534
8	0.7634231082297533	1.3122283134845636
9	0.5845582656256194	1.130379911365062
>10	5.710684590327236	25.600474932814084
>50	0.7864057344791557	11.633144362242179
>100	0.3247545917480631	12.401115670815562
>500	0.024981122442158697	3.4804536995479833
>1k	0.022982632646786003	9.397074700852242
>5k	0.002997734693059044	4.958857510237479
>10k+	9.992448976863478E-4	2.4363819987952944
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	11348	2.394370654822817	No Hit
GTACATAAGCAGTGGTATCAACGCA	8386	1.7694036227832344	No Hit
GGTATCAACGCAGAGTACTTTTTTT	7884	1.6634841595543786	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6827	1.440462500923103	No Hit
GCTTATGTACTCTGCGTTGATACCA	4712	0.9942081887138804	No Hit
CTTATGTACTCTGCGTTGATACCAC	3762	0.7937629893764043	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3761	0.7935519944297333	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3237	0.6829906423741152	No Hit
GAGTACATAAGCAGTGGTATCAACG	2438	0.5144056799839644	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2012	0.42452183270210675	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	1841	0.3884416968213611	No Hit
GCGTTGATACCACTGCTTATGTACT	1787	0.37704796970112564	No Hit
CATAAGCAGTGGTATCAACGCAGAG	1764	0.372195085927692	No Hit
TATGTACTCTGCGTTGATACCACTG	1740	0.3671312072075874	No Hit
GTGGTATCAACGCAGAGTACATGGG	1724	0.36375528806085095	No Hit
GTACTGGTTCACTATCGGTCAGTCA	1482	0.3126945109664624	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1465	0.3091075968730549	No Hit
ATACAGGGTGACAGCCCCGTACACA	1461	0.3082636170863708	No Hit
CTGCTTATGTACTCTGCGTTGATAC	1457	0.3074196372996867	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	1399	0.2951819303927671	No Hit
ACTCTGCGTTGATACCACTGCTTAT	1175	0.247919062338457	No Hit
GGATACCACGTGTCCCGCCCTACTC	1173	0.24749707244511493	No Hit
GTATCAACGCAGAGTACATAAGCAG	1149	0.24243319372501027	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	1084	0.22871852219139352	No Hit
GGTATCAACGCAGAGTACATGGGGG	1064	0.22449862325797298	No Hit
GTGGTATCAACGCAGAGTACTTTTT	1055	0.22259966873793371	No Hit
GGTATCAACGCAGAGTACATAAGCA	1027	0.21669181023114495	No Hit
TATCAACGCAGAGTACATAAGCAGT	997	0.21036196183101416	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	877	0.18504256823049087	No Hit
CTTATACACATCTCCGAGCCCACGA	858	0.18103366424374137	No Hit
CCCATGTACTCTGCGTTGATACCAC	830	0.17512580573695258	No Hit
ACATAAGCAGTGGTATCAACGCAGA	768	0.16204411904334892	No Hit
CCCATATTCAGACAGGATACCACGT	766	0.16162212915000687	No Hit
GTGGTATCAACGCAGAGTACATAAG	751	0.15845720494994145	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	687	0.14495352836299571	No Hit
GCCTTGGAGGATGGTCCCCCCATAT	680	0.14347656373629852	No Hit
GTTCACTATCGGTCAGTCAGGAGTA	669	0.14115561932291723	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	637	0.13440378102944434	No Hit
TCTAAGTACCCCGAGGAAAAGAAAT	609	0.12849592252265557	No Hit
GTATCAACGCAGAGTACATGGGGGT	594	0.12533099832259018	No Hit
GATACCACTGCTTATGTACTCTGCG	576	0.12153308928251169	No Hit
CTATCGGTCAGTCAGGAGTATTTAG	569	0.1200561246558145	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG	562	0.1185791600291173	No Hit
CCCCAGTAGCGGCGAGCGAACGGGG	557	0.11752418529576217	No Hit
GGTTAATGAGGCGAACCGGGGGAAC	551	0.11625821561573599	No Hit
GTTAATGATAGTGTGTCGAAACACA	546	0.11520324088238086	No Hit
CATGTACTCTGCGTTGATACCACTG	525	0.11077234700228929	No Hit
CATCTAAGTACCCCGAGGAAAAGAA	524	0.11056135205561826	No Hit
CACACACACTGATTCAGGCTCTGGG	522	0.11013936216227621	No Hit
CCACTGCTTATGTACTCTGCGTTGA	521	0.10992836721560519	No Hit
GTCAGGAGTATTTAGCCTTGGAGGA	520	0.10971737226893416	No Hit
CAACGCAGAGTACTTTTTTTTTTTT	515	0.10866239753557902	No Hit
GCCCAGAGCCTGAATCAGTGTGTGT	488	0.10296553397546129	No Hit
TATTCAGACAGGATACCACGTGTCC	485	0.10233254913544822	No Hit
ATACCACTGCTTATGTACTCTGCGT	475	0.10022259966873794	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0010549747333551361	0.0	0.0	0.0	0.0
2	0.0010549747333551361	0.0	0.0	0.0	0.0
3	0.0010549747333551361	0.0	0.0	0.0	0.0
4	0.0010549747333551361	0.0	0.0	0.0	0.0
5	0.0010549747333551361	0.0	0.0	0.0	0.0
6	0.0012659696800261635	0.0	0.0	0.0	0.0
7	0.0012659696800261635	0.0	0.0	0.0	0.0
8	0.0012659696800261635	0.0	0.0	0.0	0.0
9	0.0012659696800261635	0.0	0.0	0.0	0.0
10	0.0012659696800261635	0.0	0.0	0.0	0.0
11	0.0012659696800261635	0.0	0.0	0.0	0.0
12	0.0012659696800261635	0.0	0.0	2.1099494667102723E-4	0.0
13	0.0012659696800261635	0.0	0.0	2.1099494667102723E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	55	2.8068825E-8	19.011723	1
GTACAAA	40	1.2753288E-5	19.01172	1
ACTAGAG	25	0.0060192617	19.003695	3
AGGACTG	45	3.5246325E-5	16.892172	5
TATACTT	40	2.7519526E-4	16.628231	5
GCTTGTC	40	2.7541697E-4	16.626476	2
GACCGTT	75	3.263267E-9	16.466393	7
CGTTATT	75	3.263267E-9	16.466393	10
TACGGCC	75	3.268724E-9	16.464655	19
ACACGTA	35	0.0021643932	16.288881	5
AGTACAC	35	0.00216592	16.287163	2
CGTAATA	35	0.002167448	16.285442	8
ACGTAAT	35	0.002167448	16.285442	7
GGACAGT	35	0.002167448	16.285442	6
GGTTTAT	65	1.8917308E-7	16.086842	1
CGAATGG	55	1.1263126E-5	15.543557	19
CCGAATG	55	1.1263126E-5	15.543557	18
TGTTACG	80	7.692506E-9	15.433986	16
GTAGGAC	50	8.6791726E-5	15.202955	3
TAGGACC	95	3.0195224E-10	15.002917	4
>>END_MODULE