FastQCFastQC Report
Wed 25 May 2016
SRR1295178_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295178_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2628357
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA199880.76047508005952No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA189010.7191184454775359No Hit
GTATCAACGCAGAGTACTTTTTTTT127640.485626572037208No Hit
GCTTATGTACTCTGCGTTGATACCA115680.4401228600224399No Hit
GAGTAAGCAGTGGTATCAACGCAGA114470.4355192236062301No Hit
GGTATCAACGCAGAGTACTTTTTTT90560.34454984615864587No Hit
TATCAACGCAGAGTACTTTTTTTTT80230.3052477270020777No Hit
CTTATGTACTCTGCGTTGATACCAC76810.2922357959744434No Hit
GCTTACTCTGCGTTGATACCACTGC72430.27557139307940287No Hit
GTGGTATCAACGCAGAGTACATGGG59450.22618692970551565No Hit
CCCATGTACTCTGCGTTGATACCAC59360.22584451046794635No Hit
GAGTACATAAGCAGTGGTATCAACG54470.20723973189334632No Hit
GCGTTGATACCACTGCTTACTCTGC50700.19289617049738678No Hit
TATGTACTCTGCGTTGATACCACTG46110.17543278938135118No Hit
GCGTTGATACCACTGCTTATGTACT38500.14647934051576708No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA37700.1434356139595953No Hit
CTGCTTACTCTGCGTTGATACCACT33470.127341909793837No Hit
ACGCAGAGTACTTTTTTTTTTTTTT32830.12490692854889955No Hit
CATAAGCAGTGGTATCAACGCAGAG32440.1234231118527658No Hit
CATGTACTCTGCGTTGATACCACTG29440.11200913726712163No Hit
ACGCAGAGTAAGCAGTGGTATCAAC29020.11041118082513145No Hit
ACGCAGAGTACATAAGCAGTGGTAT28270.1075576871787204No Hit
CTGCTTATGTACTCTGCGTTGATAC27810.10580754440892162No Hit
GTATCAACGCAGAGTAAGCAGTGGT27740.10554121833525659No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN27350.10405740163912285No Hit
ACTCTGCGTTGATACCACTGCTTAC27090.10306819050836702No Hit
ACTCTGCGTTGATACCACTGCTTAT26900.10234530545127621No Hit
GTACATGGGAAGCAGTGGTATCAAC26560.10105172166490321No Hit
TGCTTACTCTGCGTTGATACCACTG26440.10059516268147745No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTGT400.005285491214.2479236
TAGATCG655.4295397E-513.1604765
ACCGTCC802.0037405E-613.0608458
CGAGCTA751.4814263E-512.66506310
CCCGCTG550.003072323712.0893778
AGGACTA951.0343501E-612.0060485
TTAGGAC1701.8189894E-1211.740763
CTCGGAA658.0364646E-411.69060212
GGACCGT1104.981939E-711.22563656
GGACCGC600.00588302411.08192910
CGCGTAA600.00588302411.08192910
GAGAGAC600.00588386111.0817187
AATCGCC1158.8661E-710.73510418
CTAGTAC1252.227589E-710.6449573
TTAGACC909.47135E-510.5608764
GTCTAAC1002.3837645E-510.4564671
ACAGGGT5550.010.4463043
ATTAGAC1553.6379788E-910.4242083
GCCCCGT4700.010.30720514
GTACTAG1409.6089934E-810.1848691
Produced by FastQC (version 0.11.5)