FastQCFastQC Report
Wed 25 May 2016
SRR1295178_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295178_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2628357
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT319461.2154361070432975No Hit
GGTATCAACGCAGAGTACTTTTTTT231890.8822621888883435No Hit
GTACATAAGCAGTGGTATCAACGCA215500.8199038410687741No Hit
TATCAACGCAGAGTACTTTTTTTTT202400.7700628187114612No Hit
GAGTAAGCAGTGGTATCAACGCAGA140390.5341359640261958No Hit
GCTTATGTACTCTGCGTTGATACCA128600.4892790439046142No Hit
GTACTTTTTTTTTTTTTTTTTTTTT110780.4214800348658877No Hit
ACGCAGAGTACTTTTTTTTTTTTTT107530.40911489573143983No Hit
CTTATGTACTCTGCGTTGATACCAC90080.34272361022494285No Hit
GCTTACTCTGCGTTGATACCACTGC78050.29695357213650964No Hit
CCCATGTACTCTGCGTTGATACCAC69950.2661358407552703No Hit
GAGTACTTTTTTTTTTTTTTTTTTT67750.2577655927257979No Hit
GTGGTATCAACGCAGAGTACATGGG63900.2431176586742212No Hit
GAGTACATAAGCAGTGGTATCAACG56610.21538170043110583No Hit
GCGTTGATACCACTGCTTACTCTGC55710.21195750805541258No Hit
GTGGTATCAACGCAGAGTACTTTTT48190.1833464784273978No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA43460.16535044516403213No Hit
TATGTACTCTGCGTTGATACCACTG41510.1579313616833634No Hit
GCAGAGTACTTTTTTTTTTTTTTTT41190.15671387106089468No Hit
CATAAGCAGTGGTATCAACGCAGAG41050.15618121891356462No Hit
GCGTTGATACCACTGCTTATGTACT40820.15530614752866523No Hit
CTGCTTACTCTGCGTTGATACCACT37730.14354975370545173No Hit
ACGCAGAGTAAGCAGTGGTATCAAC37060.14100063271465785No Hit
CATGTACTCTGCGTTGATACCACTG34620.13171726671833392No Hit
CTGCTTATGTACTCTGCGTTGATAC32670.1242981832376652No Hit
GTATCAACGCAGAGTAAGCAGTGGT32610.12406990374595232No Hit
ACGCAGAGTACATAAGCAGTGGTAT31680.12053157162440263No Hit
TATCAACGCAGAGTAAGCAGTGGTA31210.1187433822726517No Hit
ACTCTGCGTTGATACCACTGCTTAT31130.11843900961703452No Hit
GGTATCAACGCAGAGTAAGCAGTGG30950.11775417114189587No Hit
ACTCTGCGTTGATACCACTGCTTAC30540.11619426128185782No Hit
ATCAACGCAGAGTACTTTTTTTTTT30450.1158518420442885No Hit
GTGGTATCAACGCAGAGTAAGCAGT28690.10915564362071059No Hit
GTACATGGGAAGCAGTGGTATCAAC27150.1032964700000799No Hit
AAGCAGTGGTATCAACGCAGAGTAC26960.10257358494298911No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA500.001500639413.2992816
TAGACCG604.0903097E-412.6679115
CTCGTGT604.0909662E-412.6676698
GACCCAA550.003063665712.0939371
TGGACCG802.865247E-511.8761655
ACCGTCC802.8658049E-511.8759398
TAGACAG2350.011.7245555
CTCGGAA658.021334E-411.69323312
ACAGGGT4600.011.5659133
GTCCTAA1001.9252511E-611.4028551
TCGTGTG1501.7644197E-1011.4009029
TTAGACA2450.011.2451464
TACACAT3400.011.1775675
CGTACGA600.005874028411.0842110
CGCCCTA4500.010.97572216
GTCTAGG2100.010.8598621
GGACCGT1053.4708319E-610.8565566
TGTCCCG4650.010.82881411
CCCGCCC4650.010.82819614
CCCTAGA803.7638238E-410.6901771
Produced by FastQC (version 0.11.5)