FastQCFastQC Report
Wed 25 May 2016
SRR1295177_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295177_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4702239
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65320.13891254783093757No Hit
GTGGTATCAACGCAGAGTACATGGG65210.1386786167185462No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN52010.11060688323158392No Hit
GGTATCAACGCAGAGTACTTTTTTT51470.10945849413439002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG2850.012.6740043
CGACTTG855.3370903E-511.17458614
GGTATCA36550.011.028671
TACGGTC1408.290954E-910.8635465
TGGTATC16000.010.8120472
GTGGTAT16950.010.5446661
TAGTACG3200.010.3961992
CCGGTCG1554.078902E-89.8051129
TAGACTG4400.09.5056045
GCTAGAC2252.7284841E-119.2942693
ACGGTCT1356.8694517E-69.14660556
GTACGGG2600.09.1401014
ACGGTCC1054.502848E-49.046198
AATACGC850.0074035218.9464515
CTACACT6600.08.9296034
CTAGACT3050.08.726554
CGTTCCG1107.160339E-48.63510
AGCGCAA1651.0536787E-68.63510
TACCGTG1451.6939426E-58.5159877
TACACTG9500.08.5050145
Produced by FastQC (version 0.11.5)