##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295177_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4702239
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.195773757990608	31.0	30.0	33.0	26.0	34.0
2	30.43150741593526	31.0	30.0	34.0	26.0	34.0
3	30.367708446976003	31.0	30.0	34.0	26.0	34.0
4	33.519102282976256	35.0	35.0	37.0	27.0	37.0
5	34.002082412229576	35.0	35.0	37.0	28.0	37.0
6	34.14888481848753	37.0	35.0	37.0	29.0	37.0
7	34.07362641499082	37.0	35.0	37.0	29.0	37.0
8	34.065217654823584	37.0	35.0	37.0	29.0	37.0
9	35.08649773012388	39.0	35.0	39.0	27.0	39.0
10	35.106418027667246	39.0	35.0	39.0	27.0	39.0
11	35.56179130835332	39.0	35.0	39.0	28.0	39.0
12	35.542402034435085	39.0	35.0	39.0	28.0	39.0
13	35.49625486922294	39.0	35.0	39.0	28.0	39.0
14	36.16271291187028	39.0	36.0	41.0	27.0	41.0
15	36.517332487778695	40.0	36.0	41.0	27.0	41.0
16	36.48582324292747	40.0	36.0	41.0	27.0	41.0
17	36.14763732766455	39.0	36.0	41.0	27.0	41.0
18	36.045834760844784	39.0	36.0	41.0	27.0	41.0
19	36.464717339973575	40.0	36.0	41.0	27.0	41.0
20	36.485583782534235	40.0	36.0	41.0	27.0	41.0
21	36.53764174896257	40.0	36.0	41.0	27.0	41.0
22	36.4417942176057	40.0	36.0	41.0	27.0	41.0
23	36.13793046248819	39.0	36.0	41.0	27.0	41.0
24	36.41388091928122	40.0	36.0	41.0	27.0	41.0
25	35.135790630803754	39.0	34.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.09074247115916378
1101	2	0.07507726472128695
1101	3	-0.14829726831175094
1101	4	-0.20826423359704904
1101	5	-0.28164713542404485
1101	6	-0.3357404209750996
1101	7	-0.23485399312900057
1101	8	-0.2382115116041561
1101	9	-0.07403281809544637
1101	10	-0.05315608492568202
1101	11	-0.00985109003170237
1101	12	-0.03553757620349529
1101	13	-0.015388699013236362
1101	14	0.33887583015703626
1101	15	0.12626530890621268
1101	16	0.03128280140418127
1101	17	0.11097672862585028
1101	18	0.2539509486242366
1101	19	0.01242872649272897
1101	20	0.05209813946868991
1101	21	0.09424460785008648
1101	22	0.0495945152090016
1101	23	0.09843450160420986
1101	24	-0.04870742745977452
1101	25	-0.13783608036939654
1102	1	-0.23539530917489415
1102	2	0.04617112089545827
1102	3	-0.19278540814588396
1102	4	-0.21241160174043472
1102	5	-0.338881308697367
1102	6	-0.3325880880573564
1102	7	-0.27418786613093715
1102	8	-0.2520752430392861
1102	9	-0.13716517212538548
1102	10	0.22461879780449578
1102	11	0.10163298365991835
1102	12	0.10120761775120712
1102	13	-0.04702942208757577
1102	14	0.2126888294805127
1102	15	0.1702833462651725
1102	16	-0.007532240542509783
1102	17	-0.19409156379077075
1102	18	0.24872626584950552
1102	19	-0.022040769688089767
1102	20	-0.022537762688536134
1102	21	0.030761720776723678
1102	22	-0.07582853282809765
1102	23	0.02025958221627633
1102	24	-0.07334729656872696
1102	25	-0.04523338815868527
1103	1	-0.5112263899923555
1103	2	-0.2547028427133782
1103	3	-0.5954513796070948
1103	4	-0.5420265840910687
1103	5	-0.5472558592494394
1103	6	-0.7215439211779326
1103	7	-0.6953646753363998
1103	8	-0.5761065300226988
1103	9	-0.12951427295573126
1103	10	0.010491193949768274
1103	11	-0.2206219187706111
1103	12	-0.38323900258515664
1103	13	-0.2466228227237366
1103	14	-0.3065236193288001
1103	15	-0.35469144768865846
1103	16	-0.41587294346435755
1103	17	-0.2940041041153165
1103	18	0.04737568037790396
1103	19	-0.28474396669772517
1103	20	-0.30968890792943427
1103	21	-0.4132227955580916
1103	22	-0.2748873017600104
1103	23	-2.658615047393454
1103	24	-1.1967728684020216
1103	25	-0.7486770166246473
1104	1	-0.36819355585676306
1104	2	-0.2011356215093656
1104	3	-0.3958699378006756
1104	4	-0.7444388355257416
1104	5	-0.425052465925269
1104	6	-0.5785675970275719
1104	7	-0.45729755789415094
1104	8	-0.459030609949302
1104	9	-2.7942429237103994
1104	10	-0.8934742872707488
1104	11	-0.628178290756189
1104	12	-0.38537778761523356
1104	13	-0.3745113476506603
1104	14	-0.19866050380666422
1104	15	-0.3456197696198444
1104	16	-0.31753688740365504
1104	17	0.0019912681798075482
1104	18	0.0716916737343567
1104	19	-0.3304052924866099
1104	20	-0.2866245539739438
1104	21	-0.3006217326463201
1104	22	-0.2785096005477783
1104	23	-0.21926676910509713
1104	24	-0.3277852688064513
1104	25	-0.3628669446761634
1105	1	-0.35225050496058685
1105	2	-0.22975101081478044
1105	3	-0.2882217902691444
1105	4	-0.19599584996142028
1105	5	-0.28031124518800965
1105	6	-0.36126832304675816
1105	7	-0.41355145373712787
1105	8	-0.45274343739870204
1105	9	-2.2564829640411546
1105	10	-0.7519706070945418
1105	11	-0.5725857789924689
1105	12	-0.4190967636924583
1105	13	-0.37230908641791416
1105	14	-0.29264312540725257
1105	15	-0.3881334256795981
1105	16	-0.36586069942107713
1105	17	-1.7074857092408777
1105	18	-0.4407122532843246
1105	19	-0.39771562586571463
1105	20	-0.3881751928442796
1105	21	-0.3439420110117979
1105	22	-0.27550614416564656
1105	23	-0.21048095526906252
1105	24	-0.30303057582524673
1105	25	-0.8289946379987754
1106	1	-0.3110372427306487
1106	2	-0.3708708657085005
1106	3	-0.46243018097743516
1106	4	-0.4517755645438868
1106	5	-0.258193838974087
1106	6	-0.46154229034420524
1106	7	-0.37727482707387594
1106	8	-0.4957240424527427
1106	9	-3.216156345815346
1106	10	-0.8029124554364415
1106	11	-0.6625316213845807
1106	12	-0.40007572262880586
1106	13	-0.4862823245205945
1106	14	-0.6929083296919885
1106	15	-0.5764219941983484
1106	16	-0.6155098344694423
1106	17	-0.543667537583552
1106	18	-0.11644097857021762
1106	19	-0.3231267981048589
1106	20	-0.34780995923186
1106	21	-0.3294694887942171
1106	22	-0.2960857105375041
1106	23	-0.11157009602028012
1106	24	-0.36598618808003636
1106	25	-0.7314537190420367
1107	1	-0.308812955952817
1107	2	-0.11293776682597567
1107	3	-0.23175196513037832
1107	4	-1.5723536372952616
1107	5	-0.49757892514355717
1107	6	-0.301434037442057
1107	7	-0.08356637070729533
1107	8	-0.09098980742486162
1107	9	0.22706346882356598
1107	10	0.4147665420231803
1107	11	0.0844278635597675
1107	12	-0.4769370034423588
1107	13	-0.18619592005091334
1107	14	0.16240787187654604
1107	15	-0.5272006466352863
1107	16	-0.8719046356329088
1107	17	-0.08478138297763849
1107	18	-0.07867586845587482
1107	19	0.08159799585073557
1107	20	-0.3197602349326232
1107	21	-0.03641311945409598
1107	22	-0.04681031750016018
1107	23	0.22304520183426746
1107	24	-0.0747152635805719
1107	25	-0.30825645960992887
1108	1	0.3189964718145397
1108	2	0.39597087334877656
1108	3	0.3618372731817523
1108	4	0.8045244578503699
1108	5	0.5776056203364064
1108	6	0.44648351636013217
1108	7	0.37810286489736455
1108	8	0.4180772353295197
1108	9	0.7588948276835623
1108	10	0.8070268715880431
1108	11	0.6060463683409125
1108	12	0.4747746036274023
1108	13	0.434844754798668
1108	14	0.5126028319936395
1108	15	0.49145193958430866
1108	16	0.5107795650257145
1108	17	0.6637994772519633
1108	18	0.6967561364318584
1108	19	0.5796985473304019
1108	20	0.5806023025843032
1108	21	0.46045200739903436
1108	22	0.46634441126656867
1108	23	0.7202603822891334
1108	24	0.5375134890635138
1108	25	0.661722786712275
1109	1	0.37919490289559477
1109	2	0.2890077309461887
1109	3	0.367402922021256
1109	4	0.6825211078072968
1109	5	0.5162830337449407
1109	6	0.4117517256177763
1109	7	0.4333729434780622
1109	8	0.35222800593663095
1109	9	0.7621264896577387
1109	10	0.472717094474028
1109	11	0.3766891673747921
1109	12	0.42054707532410873
1109	13	0.37843651920768195
1109	14	0.7677215645371618
1109	15	0.538758059780271
1109	16	0.535201532052568
1109	17	0.857143434413409
1109	18	0.6309703875233836
1109	19	0.46976417436055584
1109	20	0.46423759165579526
1109	21	0.4182463107677421
1109	22	0.4369826168259223
1109	23	0.7352391622966934
1109	24	0.5328014228902447
1109	25	0.5749781259596674
1110	1	0.39506331053231847
1110	2	0.35806334226115055
1110	3	0.4654145532518754
1110	4	0.6876915286638265
1110	5	0.5894983221495522
1110	6	0.5057003817989596
1110	7	0.4159292713083289
1110	8	0.573602504339128
1110	9	1.0944920756392094
1110	10	0.998805501734509
1110	11	0.6128802126630291
1110	12	0.5088499210077089
1110	13	0.5526793935625278
1110	14	0.8239356803719176
1110	15	0.5561899589110055
1110	16	0.6297305777798528
1110	17	1.0350990713684212
1110	18	1.1662926162213694
1110	19	0.6983428683762725
1110	20	0.8445246945876761
1110	21	0.712413668878817
1110	22	0.7392807831875103
1110	23	0.8777400440778109
1110	24	0.672238619498458
1110	25	0.6865011634122382
1111	1	0.20945237420014706
1111	2	0.21961553718326243
1111	3	0.3343345923838754
1111	4	0.7060017646930206
1111	5	0.5411073063940961
1111	6	0.4127916363894215
1111	7	0.33228936975182677
1111	8	0.2543603234740033
1111	9	0.731903321002676
1111	10	0.4850187552480989
1111	11	0.30433416984141104
1111	12	0.14868744293833913
1111	13	0.2000472098378694
1111	14	0.21599812182016365
1111	15	0.22587130040101044
1111	16	0.12425554086998858
1111	17	0.43192016289687984
1111	18	0.5481186040849906
1111	19	0.40444795824950575
1111	20	-0.09074440615574275
1111	21	-0.16640476209891375
1111	22	-0.04115990900263
1111	23	0.22044697581539197
1111	24	0.2546551221973914
1111	25	0.11045771272856086
1112	1	0.28473237746342406
1112	2	0.12434549792898508
1112	3	0.2808817023876067
1112	4	0.7304111904330881
1112	5	0.5166950551516365
1112	6	0.4638289543195455
1112	7	0.4036956271201504
1112	8	0.46386204451791
1112	9	-1.2005576581386919
1112	10	0.13069056216528452
1112	11	0.17434053026188678
1112	12	0.1597814025346196
1112	13	0.14118468091916725
1112	14	0.5708161819092652
1112	15	0.28815592402236234
1112	16	0.28843883235458634
1112	17	0.3988787558210092
1112	18	0.4658859693499693
1112	19	0.32397976034294373
1112	20	0.197073785426511
1112	21	0.17383710340935465
1112	22	0.25935310387545485
1112	23	0.01708145911567982
1112	24	0.17845171614009558
1112	25	0.27400485486791126
1113	1	0.2952744707423349
1113	2	0.2033230989332253
1113	3	0.3427724669443428
1113	4	0.5686410797653352
1113	5	0.5032822601200095
1113	6	0.3944741805923755
1113	7	0.3210393331670076
1113	8	0.430898276991897
1113	9	0.23243861944033029
1113	10	0.5374373158024639
1113	11	0.31155503518257177
1113	12	0.14091702889395208
1113	13	0.21644614141872864
1113	14	-0.09565458342284927
1113	15	0.18523831237353505
1113	16	0.20571110026580897
1113	17	0.6876223427234649
1113	18	0.41759959954715953
1113	19	0.2681948478093261
1113	20	0.29722395211609864
1113	21	0.30660097809075637
1113	22	0.3040455580861803
1113	23	0.01397970105701063
1113	24	0.16982265179071732
1113	25	0.1297243092912268
1114	1	0.43013854597486656
1114	2	0.3759612678366828
1114	3	0.4371004551622981
1114	4	0.8533744080544423
1114	5	0.6531451797906769
1114	6	0.6048968613992045
1114	7	0.3825573093586456
1114	8	0.43338062698837376
1114	9	0.14878900093688685
1114	10	0.4426844473314091
1114	11	0.44848947178424226
1114	12	0.37650511881558657
1114	13	0.47685887431259744
1114	14	0.8139170889384317
1114	15	0.603757372623484
1114	16	0.41954346618381066
1114	17	0.8464965462767609
1114	18	0.6160675085243383
1114	19	0.4292392104044396
1114	20	0.42811950525097586
1114	21	0.5295851053889606
1114	22	0.3768359306200679
1114	23	0.7620100648830501
1114	24	0.581870247073411
1114	25	0.4788237823872521
1115	1	0.48256661080389307
1115	2	0.38657557339776716
1115	3	0.6447906820900613
1115	4	0.8949248373828453
1115	5	0.7365385216185771
1115	6	0.6235732620883709
1115	7	0.6019936071895842
1115	8	0.5601520376217763
1115	9	0.8568316149361976
1115	10	0.44794872603463176
1115	11	0.45347362843867955
1115	12	0.5032165684972085
1115	13	0.35745254364557866
1115	14	0.48870942493609704
1115	15	0.5143752191706881
1115	16	0.566234787394194
1115	17	0.8524365029428509
1115	18	0.9648555537292296
1115	19	0.6140016853536707
1115	20	0.7494068488195467
1115	21	0.662699540361487
1115	22	0.0726514739212476
1115	23	0.5367640966152294
1115	24	0.6127713032875732
1115	25	0.5886235052014399
1116	1	0.6718010560983316
1116	2	0.44245010868324286
1116	3	0.7378048545905003
1116	4	1.0932069787548855
1116	5	0.8351201760136533
1116	6	0.7490064885448646
1116	7	0.6686833067266988
1116	8	0.5662052742207777
1116	9	0.9291623927425547
1116	10	0.7991317327919703
1116	11	0.5681639827785787
1116	12	0.4863437891280711
1116	13	0.4277129902224175
1116	14	0.8560665299356955
1116	15	0.6258674477102701
1116	16	0.6854614918405773
1116	17	1.0235509432185737
1116	18	1.0533844650495467
1116	19	0.7236249386182507
1116	20	0.6557394013605631
1116	21	0.7285237092693038
1116	22	0.7665911497118927
1116	23	1.0029227507980139
1116	24	0.6332054950676564
1116	25	0.6599875620214206
1201	1	-0.323261177538285
1201	2	-0.019398151417362897
1201	3	-0.34752610295001674
1201	4	-0.39176589587417965
1201	5	-0.4545461212479225
1201	6	-0.4614793087162212
1201	7	-0.3425433736969268
1201	8	-0.37941017310640746
1201	9	0.044065044505188666
1201	10	0.1551671489421551
1201	11	-0.03148265393868144
1201	12	-0.059101730448496426
1201	13	-0.025065681504422344
1201	14	0.34469861259909607
1201	15	0.04465437502151559
1201	16	-0.04988497606812814
1201	17	-0.0409570546691711
1201	18	0.18722303446075017
1201	19	-0.0860512293895539
1201	20	-0.012976309181830459
1201	21	-0.11622357625788027
1201	22	-0.1372407840632377
1201	23	-0.24636870658250842
1201	24	-0.25279722295983476
1201	25	-0.22742382967707186
1202	1	-0.490390620028613
1202	2	-0.33507294680622834
1202	3	-0.5833147242005268
1202	4	-0.5170265840910702
1202	5	-0.6891993271306376
1202	6	-0.6529601137049852
1202	7	-0.51581207507909
1202	8	-0.47479917747610045
1202	9	0.040972686198315955
1202	10	0.18878202098226637
1202	11	-0.15804099397477245
1202	12	-0.18233326406254946
1202	13	-0.14823908267378272
1202	14	-0.26077505206747986
1202	15	-0.2942587709268949
1202	16	-0.17500480358227577
1202	17	0.237256946720251
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2211	9	-1.349267406509334
2211	10	0.1459092407145448
2211	11	0.19783813594490596
2211	12	0.08236730159981676
2211	13	0.11422904777783316
2211	14	0.43376128107369283
2211	15	0.2172083790294863
2211	16	0.36745903126772106
2211	17	0.5953709289898441
2211	18	0.6701323889445234
2211	19	0.30244583368075695
2211	20	0.42504949425918426
2211	21	0.2940562975979262
2211	22	0.37647381032562777
2211	23	-1.8558536817481084
2211	24	-0.4272051925181941
2211	25	-0.05838327671526855
2212	1	0.07021470723124068
2212	2	-1.4438181382549011
2212	3	-0.24304255554148568
2212	4	0.45649387135709674
2212	5	0.39754085825749286
2212	6	0.329624627361774
2212	7	0.2919312779376071
2212	8	0.284657664780795
2212	9	0.7484649010904292
2212	10	0.6167404011230886
2212	11	0.3841309515219038
2212	12	0.22488872725139686
2212	13	0.09116755979477631
2212	14	0.5385611325433146
2212	15	0.3062629735288027
2212	16	0.24940164663991737
2212	17	0.6342495872510412
2212	18	0.7753518390832639
2212	19	0.48796948650522864
2212	20	0.4354828035988021
2212	21	0.39603227316048617
2212	22	0.4235806278376728
2212	23	0.5996201256212572
2212	24	0.3915415422236208
2212	25	0.3325459362882057
2213	1	0.34456748117503366
2213	2	0.23624093171525118
2213	3	0.3066611217171413
2213	4	0.6101033413615369
2213	5	0.4581918990319025
2213	6	0.42810160235439554
2213	7	0.2820873874016172
2213	8	0.40389642510947255
2213	9	0.655922082975998
2213	10	0.6251323405410503
2213	11	0.3938208715432481
2213	12	0.35143557045400087
2213	13	0.21781467065509474
2213	14	-1.137421895873409
2213	15	-0.15236511746100234
2213	16	0.12786524297660407
2213	17	0.5302094404263329
2213	18	0.28637520086589063
2213	19	0.20454214585378594
2213	20	0.27237762157449197
2213	21	0.3487164959907858
2213	22	0.3481030689696851
2213	23	0.6041624144267956
2213	24	0.39964291869464574
2213	25	0.3339617212551289
2214	1	0.30410425668740615
2214	2	0.28216466254892225
2214	3	0.37615208325329164
2214	4	0.6562154985712993
2214	5	0.4784478416439555
2214	6	0.4380082423369771
2214	7	0.1772078516636313
2214	8	0.22967448241853106
2214	9	0.3994724989288301
2214	10	0.24543089881132119
2214	11	0.20707211185611385
2214	12	0.0329795606172425
2214	13	0.06084563860503778
2214	14	0.2982248160327359
2214	15	0.14137145260445294
2214	16	0.18914240081230815
2214	17	0.3155349914716581
2214	18	0.4064954078905316
2214	19	0.19392917491646244
2214	20	0.29927821990862213
2214	21	0.29720449758222856
2214	22	0.3047199859283154
2214	23	0.35072079273379586
2214	24	0.27760457832157925
2214	25	0.24772396361046845
2215	1	0.30979428182434177
2215	2	0.1673576296703132
2215	3	0.3694564527037727
2215	4	0.7592787432346668
2215	5	0.4541053903568155
2215	6	0.5051313188507649
2215	7	0.3859638743060785
2215	8	0.3222290189245598
2215	9	0.5602557657458149
2215	10	0.5959517479272591
2215	11	0.26998077909782126
2215	12	0.2491927979041222
2215	13	0.12259629825572205
2215	14	0.28992385882612837
2215	15	0.23506873870044132
2215	16	0.2784402254694953
2215	17	0.42361353041385286
2215	18	0.6443913316782073
2215	19	0.3327402800097303
2215	20	-0.5746622026121031
2215	21	-0.017111995897231225
2215	22	-0.6393695766173266
2215	23	0.1363472293510668
2215	24	0.21152694162874042
2215	25	0.2908260382860419
2216	1	0.3200999625381833
2216	2	-0.21503165746505815
2216	3	0.28376483587784307
2216	4	0.6661775502758545
2216	5	0.4973133873379467
2216	6	0.46707676147963895
2216	7	0.4251799896256543
2216	8	0.34135731455974394
2216	9	0.7100611968279438
2216	10	0.4427077341232035
2216	11	0.3093346541422477
2216	12	0.035584136882079065
2216	13	0.17809713344331612
2216	14	0.17618671794138407
2216	15	0.16193530934963718
2216	16	0.3049683379729302
2216	17	0.36486902385254183
2216	18	0.6618899328502721
2216	19	0.3566524644344966
2216	20	-0.8637671308010368
2216	21	-1.0516845161365822
2216	22	-1.0178523842173632
2216	23	-1.5439695666515405
2216	24	-1.1717481785471833
2216	25	-0.8892881074427237
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9433.0
3	1261.0
4	1103.0
5	867.0
6	1012.0
7	1258.0
8	580.0
9	1153.0
10	2667.0
11	5520.0
12	9747.0
13	14894.0
14	20168.0
15	24153.0
16	27912.0
17	29914.0
18	30418.0
19	30921.0
20	30593.0
21	30671.0
22	31436.0
23	32818.0
24	34867.0
25	37223.0
26	40828.0
27	45963.0
28	54063.0
29	65084.0
30	80248.0
31	102100.0
32	133134.0
33	178983.0
34	243986.0
35	345669.0
36	511656.0
37	834818.0
38	1655118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.328247859507265	20.225422854199024	13.479604330159892	23.966724956133824
2	15.885390285583764	20.721548631903477	39.93026490081761	23.462796181695143
3	18.18584551405724	26.063743861979987	29.70911584130379	26.04129478265898
4	13.800254226163094	17.36176852611795	36.798159154970165	32.03981809274879
5	13.773230498677208	37.30866599507772	35.07269747564956	13.845406030595509
6	31.294880363131377	38.171727750478624	17.09800467229493	13.435387214095067
7	26.636791682804496	32.397947588608666	22.893136115761294	18.072124612825544
8	24.751627236710146	36.523756684865766	20.49629945978964	18.22831661863445
9	25.397706102307342	17.022243714145844	21.151694900424626	36.42835528312219
10	15.658376966983012	29.40561822241111	33.11257684407092	21.823427966534954
11	32.83792038754274	25.069725100951025	23.438021231922452	18.65433327958379
12	21.63318288446564	26.80741897644552	30.644302557360454	20.91509558172839
13	26.941100901343322	23.616086752188078	26.412339556686113	23.030472789782486
14	21.591653406693098	22.83621340385975	27.20618010777809	28.365953081669065
15	23.16112322355299	30.369360110900878	23.77165432478206	22.69786234076408
16	22.927077040177217	30.121541110735656	25.134438880867783	21.81694296821935
17	21.63659476416369	29.50403024250607	27.21814874781392	21.64122624551632
18	21.664109455876325	29.200658793828644	28.045465223533512	21.089766526761526
19	22.26996387828602	28.306240091709505	28.182000345276382	21.241795684728093
20	23.056684518899498	27.910000343435236	27.712258440774168	21.321056696891098
21	23.36686569839651	28.049666881490047	27.074254911417366	21.50921250869608
22	22.861276047149204	27.56730168907728	27.90043729113701	21.67098497263651
23	23.06487035440713	27.881256059376234	27.40751040623892	21.64636317997771
24	22.716778975956885	27.458197369221104	27.588765586314235	22.236258068507777
25	23.099736766259145	27.606817239751347	27.285129778991607	22.008316214997905
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13698.0
1	13698.0
2	11786.5
3	9875.0
4	9875.0
5	9875.0
6	10185.0
7	10495.0
8	10495.0
9	10495.0
10	13992.5
11	17490.0
12	17490.0
13	17490.0
14	27086.0
15	36682.0
16	36682.0
17	36682.0
18	57487.5
19	78293.0
20	78293.0
21	78293.0
22	113570.0
23	148847.0
24	148847.0
25	148847.0
26	193437.0
27	238027.0
28	238027.0
29	238027.0
30	291336.0
31	344645.0
32	344645.0
33	344645.0
34	393992.0
35	443339.0
36	443339.0
37	443339.0
38	484199.0
39	525059.0
40	525059.0
41	525059.0
42	542145.5
43	559232.0
44	559232.0
45	559232.0
46	549384.5
47	539537.0
48	539537.0
49	539537.0
50	523786.5
51	508036.0
52	508036.0
53	508036.0
54	470228.0
55	432420.0
56	432420.0
57	432420.0
58	377853.0
59	323286.0
60	323286.0
61	323286.0
62	273488.0
63	223690.0
64	223690.0
65	223690.0
66	177832.5
67	131975.0
68	131975.0
69	131975.0
70	100092.0
71	68209.0
72	68209.0
73	68209.0
74	49682.5
75	31156.0
76	31156.0
77	31156.0
78	21718.5
79	12281.0
80	12281.0
81	12281.0
82	8329.5
83	4378.0
84	4378.0
85	4378.0
86	2798.5
87	1219.0
88	1219.0
89	1219.0
90	759.0
91	299.0
92	299.0
93	299.0
94	177.0
95	55.0
96	55.0
97	55.0
98	35.5
99	16.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1426554456291992
2	0.283949837513576
3	0.3419222204571057
4	0.3707595466755305
5	0.37909600086256784
6	0.33794539154645264
7	0.34119916065516875
8	0.3581697995359232
9	0.3453886542134502
10	0.37407711517853515
11	0.2940514082759298
12	0.3411140947961174
13	0.31219170271864105
14	0.32327153086008603
15	0.36097697288461944
16	0.310639250790953
17	0.35967971853408554
18	0.3294600721060754
19	0.3431982083428767
20	0.3043231107563865
21	0.34492079198866754
22	0.29896396163614825
23	0.281248996488694
24	0.31972003124469
25	0.31410568454729754
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4702239.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.36023518517253
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.52405552565867	51.977109720357696
2	12.636406908573806	16.51837054879846
3	3.682091253471452	7.219870509004823
4	1.55647413750416	4.069260627476418
5	0.7828529461794285	2.558372633884633
6	0.4446528323452997	1.7437568218705128
7	0.27548942037781876	1.2604237314844768
8	0.2027085522690025	1.0599262920278274
9	0.14915034544360176	0.8773651490529184
>10	0.6913928553861146	7.86396614523804
>50	0.03808411396097707	1.725663368102147
>100	0.015466001889264974	1.6606838010516896
>500	5.549118961610323E-4	0.255249582069785
>1k	4.896276664636166E-4	0.7107257356379052
>5k	1.30567377723098E-4	0.49925533394262583
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6532	0.13891254783093757	No Hit
GTGGTATCAACGCAGAGTACATGGG	6521	0.1386786167185462	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	5201	0.11060688323158392	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5147	0.10945849413439002	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.1266464762850207E-5	0.0
6	0.0	0.0	0.0	2.1266464762850207E-5	0.0
7	0.0	0.0	0.0	2.1266464762850207E-5	0.0
8	0.0	0.0	0.0	2.1266464762850207E-5	0.0
9	0.0	0.0	0.0	2.1266464762850207E-5	0.0
10	0.0	0.0	0.0	2.1266464762850207E-5	0.0
11	0.0	0.0	0.0	4.2532929525700414E-5	0.0
12	0.0	0.0	0.0	4.2532929525700414E-5	0.0
13	0.0	0.0	0.0	4.2532929525700414E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTACGG	285	0.0	12.674004	3
CGACTTG	85	5.3370903E-5	11.174586	14
GGTATCA	3655	0.0	11.02867	1
TACGGTC	140	8.290954E-9	10.863546	5
TGGTATC	1600	0.0	10.812047	2
GTGGTAT	1695	0.0	10.544666	1
TAGTACG	320	0.0	10.396199	2
CCGGTCG	155	4.078902E-8	9.805112	9
TAGACTG	440	0.0	9.505604	5
GCTAGAC	225	2.7284841E-11	9.294269	3
ACGGTCT	135	6.8694517E-6	9.1466055	6
GTACGGG	260	0.0	9.140101	4
ACGGTCC	105	4.502848E-4	9.04619	8
AATACGC	85	0.007403521	8.946451	5
CTACACT	660	0.0	8.929603	4
CTAGACT	305	0.0	8.72655	4
CGTTCCG	110	7.160339E-4	8.635	10
AGCGCAA	165	1.0536787E-6	8.635	10
TACCGTG	145	1.6939426E-5	8.515987	7
TACACTG	950	0.0	8.505014	5
>>END_MODULE