FastQCFastQC Report
Wed 25 May 2016
SRR1295177_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295177_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4702239
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT162880.34638817805730415No Hit
GGTATCAACGCAGAGTACTTTTTTT130690.27793142798568937No Hit
TATCAACGCAGAGTACTTTTTTTTT92680.19709759542209573No Hit
GTGGTATCAACGCAGAGTACATGGG69420.14763179838370616No Hit
ACGCAGAGTACTTTTTTTTTTTTTT61890.13161815041727995No Hit
GGTATCAACGCAGAGTACATGGGGG47870.10180256681976395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA3650.012.23333553
GGTATCA46600.011.643471
ATACGAC600.005877017511.0837493
GTGGTAT19450.010.7970451
TGGTATC19050.010.3729832
TACGACT750.002648454210.1337134
GTATACG1252.5649206E-69.8824721
TAGGACC2700.09.5003574
GTCCTAG5200.09.319641
GTCTTAG4400.09.0704511
TAGACAG5350.08.8788385
AGGACCG2151.0422809E-98.8375415
TTAGACT3550.08.8313174
ACGACTG1401.0857166E-58.821765
TCTAGAC4200.08.8217593
CAGCGTC5100.08.7551372
CTAGACA5550.08.7300584
GCAGCGT4750.08.6021531
GTACGGA1554.197551E-68.5827921
CGCACGA1000.0029070788.54986612
Produced by FastQC (version 0.11.5)