FastQCFastQC Report
Wed 25 May 2016
SRR1295176_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295176_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5466224
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT77590.14194442086529932No Hit
GTGGTATCAACGCAGAGTACATGGG69310.12679685281832578No Hit
GGTATCAACGCAGAGTACTTTTTTT62690.11468611604647011No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN60820.11126510732088549No Hit
ATTCCATTCCATTCCATTCCATTCC57830.10579515219281171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40200.011.0681851
TAGACAG6550.09.8685285
GTGGTAT18200.09.7162391
TGGTATC17850.09.5857722
GTACTAG3050.09.0397161
GCGAATG950.00182827038.99822113
AGAGTCG850.0074506938.9395748
TCCGCTC1304.3823187E-58.7676598
CTATACT6950.08.7537854
ATAGTAC5650.08.5805383
CTTATAC7450.08.5489213
GTACCGT1000.0029120238.548316
TTAGACA6600.08.4978464
TAGCACC4700.08.4947814
TAGGACC3250.08.4823344
TAGTACC3950.08.4230964
GTCTTAC4750.08.4064441
TCTACAC6450.08.4005543
CGCCTTA1150.00110456648.2659912
CGTCAGA2105.7569196E-88.1479052
Produced by FastQC (version 0.11.5)