##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295176_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5466224
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.025851483583548	31.0	30.0	33.0	26.0	34.0
2	30.31457986354017	31.0	30.0	34.0	26.0	34.0
3	30.313264147243142	31.0	30.0	34.0	26.0	34.0
4	33.7185181214674	35.0	35.0	37.0	28.0	37.0
5	33.78773610448456	35.0	35.0	37.0	28.0	37.0
6	34.06160980596478	36.0	35.0	37.0	29.0	37.0
7	34.00648729360524	37.0	35.0	37.0	29.0	37.0
8	34.176613142820344	37.0	35.0	37.0	30.0	37.0
9	35.18013970887399	39.0	35.0	39.0	27.0	39.0
10	35.23951287031048	39.0	35.0	39.0	27.0	39.0
11	35.611769660372495	39.0	35.0	39.0	29.0	39.0
12	35.61610848732141	39.0	35.0	39.0	29.0	39.0
13	35.53826151288348	39.0	35.0	39.0	28.0	39.0
14	36.201447470868374	39.0	36.0	41.0	27.0	41.0
15	36.55943865454471	40.0	36.0	41.0	27.0	41.0
16	36.54166239802833	40.0	36.0	41.0	27.0	41.0
17	36.2197220604205	39.0	36.0	41.0	27.0	41.0
18	36.13978552653532	39.0	36.0	41.0	27.0	41.0
19	36.57505601673111	40.0	36.0	41.0	27.0	41.0
20	36.588125916537635	40.0	36.0	41.0	27.0	41.0
21	36.63643531622561	40.0	36.0	41.0	28.0	41.0
22	36.53362485693963	40.0	36.0	41.0	27.0	41.0
23	36.25355565377489	39.0	36.0	41.0	27.0	41.0
24	36.54064213248488	40.0	36.0	41.0	27.0	41.0
25	35.28615311044699	39.0	34.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.3944740242904068
1101	2	-0.007765970841074221
1101	3	-0.27635765370132503
1101	4	-0.2028271655088716
1101	5	-0.36351989233290993
1101	6	-0.464352021938204
1101	7	-0.48773812147599926
1101	8	-0.2977607690873967
1101	9	-0.10948963716567306
1101	10	-0.04410992625951593
1101	11	-0.0697420535943607
1101	12	-0.036709855435773875
1101	13	-0.16602723676129472
1101	14	0.26427029219490805
1101	15	0.08297839470525048
1101	16	-0.022387951751156265
1101	17	0.044586733799505396
1101	18	0.23738862045737363
1101	19	-0.05350957100270648
1101	20	-0.09910775474750722
1101	21	-0.10420335960636606
1101	22	-0.16252420525853495
1101	23	-0.12055207111811939
1101	24	-0.205910474026183
1101	25	-0.3057101647001872
1102	1	-0.4016739999621457
1102	2	-0.0371360947212942
1102	3	-0.2891213145494831
1102	4	-0.3028602036857322
1102	5	-0.7504104131692699
1102	6	-0.5762746168558905
1102	7	-0.5739461948891744
1102	8	-0.45651513120652965
1102	9	-0.383000732087325
1102	10	0.0656409195001828
1102	11	-0.15615933573962337
1102	12	-0.17125324436270972
1102	13	-0.31443255662946257
1102	14	-0.10298405405796274
1102	15	-0.15210751557282265
1102	16	-0.235204549708115
1102	17	-0.39616276398935923
1102	18	-0.07675199469507987
1102	19	-0.16449379795895425
1102	20	-0.15962505095203738
1102	21	-0.22997912596866854
1102	22	-0.27350705493543614
1102	23	-0.21578162722428829
1102	24	-0.2532176780565649
1102	25	-0.3658061786995148
1103	1	-0.5783395166478975
1103	2	-0.21438048371552298
1103	3	-0.5569455492579252
1103	4	-0.41418337318336285
1103	5	-0.7484942360993401
1103	6	-0.7192162775645485
1103	7	-0.6421039212600306
1103	8	-0.5411186497108957
1103	9	-0.15145260748813882
1103	10	-0.04105134159912893
1103	11	-0.18708206467375987
1103	12	-0.41849322710542936
1103	13	-0.33908287260234005
1103	14	-0.34114347583085447
1103	15	-0.32949741092624407
1103	16	-0.43514864544459897
1103	17	-0.31937844303599405
1103	18	-0.062276739562861394
1103	19	-0.35073053040099467
1103	20	-0.34783677681665637
1103	21	-0.2825766968603105
1103	22	-0.29067670722218253
1103	23	-2.55126058726821
1103	24	-1.1782956507144817
1103	25	-0.695184928627377
1104	1	-0.4276440973074429
1104	2	-0.1673760604175314
1104	3	-0.3463909531378597
1104	4	-0.5912792626648695
1104	5	-0.5679494675671322
1104	6	-0.5731304174527168
1104	7	-0.5361007810965788
1104	8	-0.384023841582092
1104	9	-2.5915084137691053
1104	10	-0.8492838140555605
1104	11	-0.5211715815953397
1104	12	-0.44395696309422306
1104	13	-0.37165402190004926
1104	14	-0.10658860265833425
1104	15	-0.3196753217174688
1104	16	-0.3116753688151377
1104	17	-0.031404025155367776
1104	18	0.061732661844807524
1104	19	-0.28143662898290245
1104	20	-0.33754109752749173
1104	21	-0.31375890867892764
1104	22	-0.388205378087747
1104	23	-0.2500657632018246
1104	24	-0.28083494251327323
1104	25	-0.4354890714450974
1105	1	-0.3910022103945998
1105	2	-0.21951532583382516
1105	3	-0.3884489338090873
1105	4	-0.15884985390622575
1105	5	-0.42860337355464395
1105	6	-0.44038953481162224
1105	7	-0.4513211590325312
1105	8	-0.4970969639722398
1105	9	-2.295147268385449
1105	10	-0.693656617122862
1105	11	-0.4917440616694222
1105	12	-0.3897317233197555
1105	13	-0.32601509387615124
1105	14	-0.3703275315292416
1105	15	-0.32848110792645
1105	16	-0.37961528473525874
1105	17	-1.6323837900818035
1105	18	-0.42334625127170966
1105	19	-0.38967383136469635
1105	20	-0.3952272209594625
1105	21	-0.2996812039636225
1105	22	-0.2597090470535761
1105	23	-0.29645975846421635
1105	24	-0.29959521604109796
1105	25	-0.8278336238990249
1106	1	-0.4295998786133026
1106	2	-0.458187993894299
1106	3	-0.5315670683465434
1106	4	-0.4065408749518866
1106	5	-0.46442540885743
1106	6	-0.5753462648607126
1106	7	-0.5140082044653909
1106	8	-0.4992193809666716
1106	9	-3.221828945568973
1106	10	-0.9021754575660026
1106	11	-0.7371407251551361
1106	12	-0.47378736525581644
1106	13	-0.6688530074858008
1106	14	-0.8850090523605374
1106	15	-0.7646703834296176
1106	16	-0.7116602651842001
1106	17	-0.5631245597332466
1106	18	-0.22005838314954218
1106	19	-0.4445014817517432
1106	20	-0.4972127945951499
1106	21	-0.4331366186146468
1106	22	-0.34357742379634004
1106	23	-0.17029951126360743
1106	24	-0.4886452450025942
1106	25	-0.820579068177274
1107	1	-0.38937828857578793
1107	2	-0.1611680940211997
1107	3	-0.2888906493010488
1107	4	-1.5020379534218193
1107	5	-0.5900256357001794
1107	6	-0.39698999158957804
1107	7	-0.24513563152010676
1107	8	-0.22578554885819813
1107	9	0.02538200424206849
1107	10	0.27781008585718325
1107	11	-0.03352844031873303
1107	12	-0.5749915376748973
1107	13	-0.20148721890510046
1107	14	0.04155990458117742
1107	15	-0.7136249544504594
1107	16	-1.042470670200771
1107	17	-0.19736386953629648
1107	18	-0.23961194918262407
1107	19	-0.12757241194646696
1107	20	-0.48151625255254515
1107	21	-0.1708996609105924
1107	22	-0.2422837693503581
1107	23	0.025160612291315942
1107	24	-0.32339866972380094
1107	25	-0.34086243567647045
1108	1	0.32238308948159045
1108	2	0.26962821755310173
1108	3	0.36106703167920884
1108	4	0.7552138431821334
1108	5	0.612878637322126
1108	6	0.5069804132397948
1108	7	0.45489941717543303
1108	8	0.4029065380583958
1108	9	0.7846435634060924
1108	10	0.8268006834257164
1108	11	0.5138243017400654
1108	12	0.4959407419912836
1108	13	0.39764513695615733
1108	14	0.5165900673061472
1108	15	0.5828882345430486
1108	16	0.5633410810405266
1108	17	0.7364817567252615
1108	18	0.7827580305803323
1108	19	0.49560454774466933
1108	20	0.5086989594673099
1108	21	0.4496814803374747
1108	22	0.43375287725299216
1108	23	0.6584192706220335
1108	24	0.4205722510640584
1108	25	0.5959369747758103
1109	1	0.3504433464248997
1109	2	0.2184122509550157
1109	3	0.31488949788996834
1109	4	0.7699560667737728
1109	5	0.609442319202131
1109	6	0.570998711696582
1109	7	0.512650832547628
1109	8	0.4132721944614488
1109	9	0.8388856042284871
1109	10	0.58966222944332
1109	11	0.5356991911941549
1109	12	0.4648551798387146
1109	13	0.3752843325254034
1109	14	0.8754331508104158
1109	15	0.5852859978371399
1109	16	0.596888209102552
1109	17	0.8129130859882849
1109	18	0.5652901613108412
1109	19	0.39053366250067967
1109	20	0.45217107267452405
1109	21	0.41250693391536686
1109	22	0.4831216184317526
1109	23	0.715297674474904
1109	24	0.4828500633905506
1109	25	0.47346202287769756
1110	1	0.3987253752517077
1110	2	0.30406859553051646
1110	3	0.4488175505687657
1110	4	0.5368158867307145
1110	5	0.5961900796557771
1110	6	0.5611974941500719
1110	7	0.5083511938058791
1110	8	0.5502943747220144
1110	9	1.045473931158412
1110	10	0.9168378772162455
1110	11	0.6095249094038024
1110	12	0.5559178613029943
1110	13	0.5536805837752254
1110	14	0.7679031661326974
1110	15	0.6009586180036663
1110	16	0.6365983135290207
1110	17	0.9799854140745339
1110	18	1.0271459322516634
1110	19	0.5980722178111222
1110	20	0.7112761922447319
1110	21	0.6802445092458882
1110	22	0.7065471610346066
1110	23	0.8731781509230672
1110	24	0.6034203772008198
1110	25	0.6164660818219687
1111	1	0.28615484471801267
1111	2	0.1349562195791485
1111	3	0.28823420707009717
1111	4	0.6602900394839892
1111	5	0.5367353609066754
1111	6	0.44213142723319976
1111	7	0.27253248115214035
1111	8	0.23847197762574268
1111	9	0.6758720021963072
1111	10	0.517345357037172
1111	11	0.23744982727141917
1111	12	0.10665938601061242
1111	13	0.14684932139583395
1111	14	0.21690142899527842
1111	15	0.1980039305829706
1111	16	0.091852912069875
1111	17	0.44076834121313624
1111	18	0.5626472720076734
1111	19	0.3335391291117773
1111	20	-0.17777115481437988
1111	21	-0.3124150080867949
1111	22	-0.06843880676544245
1111	23	0.2588524350841155
1111	24	0.1581745107595225
1111	25	0.12227593726433383
1112	1	0.3199571359236728
1112	2	0.21217379369420186
1112	3	0.40649730388998506
1112	4	0.7707947477332837
1112	5	0.7510275787947549
1112	6	0.6562194912121413
1112	7	0.5913389418397017
1112	8	0.5370139901793962
1112	9	-1.0049017004292153
1112	10	0.32088554858216867
1112	11	0.2890619222853701
1112	12	0.3210949863532164
1112	13	0.2738733662453967
1112	14	0.6232183950190233
1112	15	0.48041956764714655
1112	16	0.3594460284059551
1112	17	0.4739069689512476
1112	18	0.5017518380927086
1112	19	0.4683265608867515
1112	20	0.44489841876818303
1112	21	0.3881258189274277
1112	22	0.5257160819520905
1112	23	0.27211933718417924
1112	24	0.38680398196372323
1112	25	0.4587344144992116
1113	1	0.4078519935770153
1113	2	0.20508400580281716
1113	3	0.41300275394613095
1113	4	0.562736915720329
1113	5	0.7495064020541164
1113	6	0.5941343251668911
1113	7	0.4838846795049534
1113	8	0.45366082530046725
1113	9	0.37407193953828255
1113	10	0.6475262419211276
1113	11	0.4417934643689847
1113	12	0.22190948592204762
1113	13	0.286787486760808
1113	14	4.917539534545767E-4
1113	15	0.22594679931641082
1113	16	0.31518524461530006
1113	17	0.6863348340305606
1113	18	0.3567388410891539
1113	19	0.3384599430234658
1113	20	0.38932143246001516
1113	21	0.32386218137942535
1113	22	0.4550841608150549
1113	23	0.12049492887354063
1113	24	0.21591398190168576
1113	25	0.28104645425329267
1114	1	0.49601898825277857
1114	2	0.4352582310741191
1114	3	0.5248562418447555
1114	4	0.7590946574548099
1114	5	0.7711271165998284
1114	6	0.6159704152161822
1114	7	0.411683234619737
1114	8	0.4342866543203243
1114	9	0.19031523797308836
1114	10	0.45410818147285426
1114	11	0.4129724462863251
1114	12	0.4186176524231726
1114	13	0.5970520944763251
1114	14	0.911212592505116
1114	15	0.5911503603230202
1114	16	0.4904578417687162
1114	17	0.7809454491864329
1114	18	0.6842263942235434
1114	19	0.531592789792839
1114	20	0.5294620342281817
1114	21	0.5865884613860928
1114	22	0.32441452869936427
1114	23	0.7300836733287497
1114	24	0.5224358927037756
1114	25	0.52018828374203
1115	1	0.4995115522495581
1115	2	0.3770530386417512
1115	3	0.5765887962150025
1115	4	0.7569293389934799
1115	5	0.7802751809797073
1115	6	0.5712745718142855
1115	7	0.5590024056576368
1115	8	0.47535486761672274
1115	9	0.7699205748034075
1115	10	0.47402025343607335
1115	11	0.41884767467083606
1115	12	0.45153372971045513
1115	13	0.38130845731792107
1115	14	0.43804160863446384
1115	15	0.48323190096072466
1115	16	0.45909961697941526
1115	17	0.7996718003386718
1115	18	0.9401911346019247
1115	19	0.5933285734709557
1115	20	0.6046061866023962
1115	21	0.3739572508008351
1115	22	-0.0734297056968174
1115	23	0.4047263375200245
1115	24	0.4537362109686427
1115	25	0.5439902596364163
1116	1	0.6959754139118388
1116	2	0.4198136171739044
1116	3	0.6488249305683382
1116	4	0.830501486176523
1116	5	0.9756233660940552
1116	6	0.7544073413576768
1116	7	0.6832590695352323
1116	8	0.5357411035717803
1116	9	0.8827669171414456
1116	10	0.6203797741150154
1116	11	0.42109127973897387
1116	12	0.40685756029456144
1116	13	0.3288697669840275
1116	14	0.8708901423821729
1116	15	0.5592377580011316
1116	16	0.5944940707207493
1116	17	0.9309818648856449
1116	18	0.9766114668993211
1116	19	0.7037235750647071
1116	20	0.6263838182862003
1116	21	0.688160872603369
1116	22	0.7243812254530795
1116	23	0.9582775168968638
1116	24	0.7487009491947916
1116	25	0.559584779120037
1201	1	-0.5483446243292782
1201	2	-0.1446009207546659
1201	3	-0.48701687306772
1201	4	-0.29153438063092096
1201	5	-0.6794396949814185
1201	6	-0.5845031019346294
1201	7	-0.6655204189372768
1201	8	-0.583923708820862
1201	9	-0.17969826925774868
1201	10	-0.030582557332529348
1201	11	-0.20852468067516838
1201	12	-0.1519450409419676
1201	13	-0.25513383650599764
1201	14	0.19770914317536636
1201	15	-0.11648722111858945
1201	16	-0.22740506307588504
1201	17	-0.1756099479429878
1201	18	-0.03534954607009411
1201	19	-0.3066913515785288
1201	20	-0.23686688691574886
1201	21	-0.2453092462697768
1201	22	-0.30773227922988156
1201	23	-0.4165428601563619
1201	24	-0.40868147775048413
1201	25	-0.42510679680276553
1202	1	-0.5850785424282208
1202	2	-0.3982915782708538
1202	3	-0.6487095253373099
1202	4	-0.49439617040816586
1202	5	-0.9716460659423589
1202	6	-0.8683134974085718
1202	7	-0.8475674050921143
1202	8	-0.6732877116682303
1202	9	-0.18154756147901452
1202	10	-0.07967948190167107
1202	11	-0.33736073690075585
1202	12	-0.3881269784227044
1202	13	-0.262619187240702
1202	14	-0.4013357885311848
1202	15	-0.38471891570775085
1202	16	-0.2965515444007423
1202	17	0.03214811404652096
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2211	11	0.1006041884696387
2211	12	0.10641786641625117
2211	13	0.04557466138304278
2211	14	0.3340785351433908
2211	15	0.17347841281969778
2211	16	0.3528911044486023
2211	17	0.5853244103529107
2211	18	0.5689940540423066
2211	19	0.2743340694517258
2211	20	0.3901609181255381
2211	21	0.2389894649483324
2211	22	0.32535543297719727
2211	23	-1.7479864343515317
2211	24	-0.4794902544290949
2211	25	-0.06815283871757316
2212	1	0.17776983054095297
2212	2	-1.3402521105490024
2212	3	-0.16036806245805124
2212	4	0.33205497584557264
2212	5	0.41974753173787605
2212	6	0.4264440791712829
2212	7	0.4275919206992853
2212	8	0.33406512132822286
2212	9	0.7440091711044019
2212	10	0.6910141711253956
2212	11	0.4372336848258982
2212	12	0.31203282177388303
2212	13	0.21203886399963778
2212	14	0.6189341699977788
2212	15	0.36567464320417287
2212	16	0.41157202529392833
2212	17	0.7564621129580047
2212	18	0.890427832604388
2212	19	0.5169568810944796
2212	20	0.5115027132637522
2212	21	0.43356209802705337
2212	22	0.42846353946727334
2212	23	0.6960735468428112
2212	24	0.39945183863528655
2212	25	0.48442614874635836
2213	1	0.36990949464610523
2213	2	0.22219997939316372
2213	3	0.3255797223727015
2213	4	0.5045888298271493
2213	5	0.6040108007796121
2213	6	0.4465997867055407
2213	7	0.3944937775375834
2213	8	0.33228813620717546
2213	9	0.7284260474460993
2213	10	0.7558060794686128
2213	11	0.4159991848132307
2213	12	0.421228109871187
2213	13	0.22956499949721376
2213	14	-1.253762366066617
2213	15	-0.22092984603433763
2213	16	0.13344903960675936
2213	17	0.5966833950206976
2213	18	0.37716615644526286
2213	19	0.3244402569038627
2213	20	0.351272518731399
2213	21	0.4061488759454761
2213	22	0.4201752869827118
2213	23	0.682650934697314
2213	24	0.3829032712090381
2213	25	0.29453184168473
2214	1	0.5253022648688273
2214	2	0.3708604619337805
2214	3	0.4310385047037535
2214	4	0.6011827750775467
2214	5	0.6465494163447687
2214	6	0.49109493557468653
2214	7	0.4089335588267744
2214	8	0.25473424494217767
2214	9	0.37903079609117896
2214	10	0.24612189062564482
2214	11	0.205811044838903
2214	12	0.08999880773394864
2214	13	0.05189732692018367
2214	14	0.21534153808502055
2214	15	0.09725104244613192
2214	16	0.27206849140139866
2214	17	0.21709285713679094
2214	18	0.46182353356254424
2214	19	0.281349908857905
2214	20	0.36428432512146713
2214	21	0.3301425222648646
2214	22	0.43084262506600624
2214	23	0.5141479775734794
2214	24	0.4146322005934451
2214	25	0.39869994458539537
2215	1	0.4076464623614946
2215	2	0.19739311944070081
2215	3	0.36981307844407496
2215	4	0.6397759652030217
2215	5	0.6076361814393465
2215	6	0.5541657932873463
2215	7	0.4704828132380712
2215	8	0.34388777376172897
2215	9	0.5996108887294653
2215	10	0.6304731371446195
2215	11	0.40466548733441243
2215	12	0.2525825759118945
2215	13	0.1731719066498556
2215	14	0.3490343771207236
2215	15	0.24693748632652301
2215	16	0.3196848213634027
2215	17	0.49501375468634023
2215	18	0.726730450877703
2215	19	0.3775936778285782
2215	20	-0.4719867429514011
2215	21	0.04127329425794102
2215	22	-0.8394993132200668
2215	23	0.15968905582207782
2215	24	0.09630361260445142
2215	25	0.31206168190777817
2216	1	0.299343148175339
2216	2	-0.26121347828959074
2216	3	0.2938931240128291
2216	4	0.45952825688026167
2216	5	0.5259592726804527
2216	6	0.4142624692043597
2216	7	0.39509521969144856
2216	8	0.27436911568800326
2216	9	0.6015046760793581
2216	10	0.2565417730723638
2216	11	0.17031083076471276
2216	12	-0.11948658493409425
2216	13	0.028429204734543134
2216	14	0.182026740487089
2216	15	-0.003720925476535797
2216	16	0.2563861747854972
2216	17	0.21725822567175612
2216	18	0.5848027870233139
2216	19	0.20230226276669327
2216	20	-0.8880048703070429
2216	21	-1.1197507878590827
2216	22	-1.1530326002779105
2216	23	-1.665507057503028
2216	24	-1.312142486079729
2216	25	-1.0133820111425393
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	10891.0
3	1495.0
4	1298.0
5	952.0
6	1151.0
7	1403.0
8	574.0
9	928.0
10	1964.0
11	4595.0
12	8709.0
13	14397.0
14	20740.0
15	26484.0
16	30765.0
17	33400.0
18	34750.0
19	35227.0
20	35823.0
21	36029.0
22	36974.0
23	38858.0
24	40588.0
25	43664.0
26	48000.0
27	53931.0
28	63100.0
29	76265.0
30	94644.0
31	118936.0
32	156502.0
33	209122.0
34	288543.0
35	410295.0
36	606191.0
37	991538.0
38	1887498.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.6266197321663	21.494903164616215	13.745965612185431	23.132511491032055
2	15.392375904155568	21.57943634777842	40.59802967197036	22.430158076095655
3	17.826082566676853	26.79974042448124	30.400699054862407	24.9734779539795
4	12.524585130795373	17.642295367519587	38.66169850086478	31.171421000820253
5	13.159433437334151	37.67417688463989	35.357333710405165	13.809055967620793
6	30.28704935263467	38.99444066512304	17.897970804222055	12.820539178020235
7	25.16194025795897	32.963047810373865	24.68182832790718	17.19318360375998
8	22.830928920354097	38.02899028608221	21.58091079018	17.559170003383688
9	24.13977117838999	17.230753697814123	22.55845139261768	36.07102373117821
10	14.631889493396324	30.09617030215823	34.34202707576322	20.929913128682227
11	31.5514361711204	26.12853680612217	24.00821911625235	18.311807906505077
12	20.223993092090048	27.94647177471167	32.084756266351036	19.744778866847245
13	25.929466299403042	24.83099695190767	27.197259836566463	22.04227691212282
14	20.39595358059005	23.75976591714417	28.420335314918194	27.423945187347588
15	22.312050747721035	31.325334385987457	24.48860287705061	21.874011989240895
16	21.764405037581472	31.65595011965623	25.7586531705277	20.820991672234598
17	19.97446844293304	30.522328361968665	28.724672469560332	20.778530725537962
18	20.015153108617284	30.456408474654978	29.412650019170812	20.115788397556926
19	20.663689806932442	29.090411788731686	29.67575480833215	20.570143596003724
20	21.960856705242097	28.81242879154679	28.859771116374223	20.366943386836887
21	22.46876317138254	29.36876860515151	27.724264128166453	20.438204095299493
22	21.21028022035387	28.843288970324743	29.526835146159762	20.419595663161626
23	21.54254187189226	29.324597425164818	28.61121221549715	20.52164848744577
24	21.191414422443067	28.25941575554345	29.08417985697066	21.464989965042818
25	21.976578492695804	28.589470323061917	28.311341070768258	21.122610113474018
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14894.0
1	14894.0
2	12887.5
3	10881.0
4	10881.0
5	10881.0
6	12122.0
7	13363.0
8	13363.0
9	13363.0
10	19301.0
11	25239.0
12	25239.0
13	25239.0
14	41090.0
15	56941.0
16	56941.0
17	56941.0
18	88872.0
19	120803.0
20	120803.0
21	120803.0
22	169853.5
23	218904.0
24	218904.0
25	218904.0
26	280117.0
27	341330.0
28	341330.0
29	341330.0
30	406286.0
31	471242.0
32	471242.0
33	471242.0
34	527159.0
35	583076.0
36	583076.0
37	583076.0
38	625160.0
39	667244.0
40	667244.0
41	667244.0
42	662638.5
43	658033.0
44	658033.0
45	658033.0
46	629539.0
47	601045.0
48	601045.0
49	601045.0
50	570522.5
51	540000.0
52	540000.0
53	540000.0
54	487910.0
55	435820.0
56	435820.0
57	435820.0
58	376238.0
59	316656.0
60	316656.0
61	316656.0
62	259712.5
63	202769.0
64	202769.0
65	202769.0
66	155966.0
67	109163.0
68	109163.0
69	109163.0
70	79479.5
71	49796.0
72	49796.0
73	49796.0
74	34583.0
75	19370.0
76	19370.0
77	19370.0
78	13119.5
79	6869.0
80	6869.0
81	6869.0
82	4497.5
83	2126.0
84	2126.0
85	2126.0
86	1316.5
87	507.0
88	507.0
89	507.0
90	314.0
91	121.0
92	121.0
93	121.0
94	70.0
95	19.0
96	19.0
97	19.0
98	16.0
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14337136568131859
2	0.28462060830291624
3	0.3451925863265025
4	0.37230453783086825
5	0.3796770860469677
6	0.33752733148147607
7	0.3391006296119588
8	0.3567910864977359
9	0.34299728660954987
10	0.37444495505489717
11	0.2927615114199491
12	0.3394665128981176
13	0.30942749510448164
14	0.3211540544258706
15	0.3599193885943935
16	0.30849449272477675
17	0.35913273952915215
18	0.3263130087607094
19	0.34116787017875594
20	0.3028598901179315
21	0.34407664230371826
22	0.2975179941400133
23	0.2801202438831632
24	0.31597680592672384
25	0.3102507324983389
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5466224.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.23938102990964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.33493307736829	61.03398916755397
2	11.831019859671557	17.329931429498263
3	2.69043145663477	5.911366037619861
4	0.8667090103378013	2.539089258007445
5	0.39918597534718453	1.4618066875124285
6	0.21860165764358172	0.9606150058756886
7	0.13764950723520764	0.7056955296285071
8	0.0842476305966324	0.49361954545070674
9	0.06671519216326253	0.43975614413959435
>10	0.330123422733329	4.372209824364684
>50	0.024377410471764713	1.228782961430331
>100	0.014726152136797087	1.903926888382159
>500	7.52736234936274E-4	0.4047264891321684
>1k	3.7636530351376813E-4	0.5152956583833755
>5k	1.5054612138873085E-4	0.6991893730208287
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7759	0.14194442086529932	No Hit
GTGGTATCAACGCAGAGTACATGGG	6931	0.12679685281832578	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6269	0.11468611604647011	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	6082	0.11126510732088549	No Hit
ATTCCATTCCATTCCATTCCATTCC	5783	0.10579515219281171	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.8294164307939082E-5	0.0
5	0.0	0.0	0.0	1.8294164307939082E-5	0.0
6	0.0	0.0	0.0	1.8294164307939082E-5	0.0
7	0.0	0.0	0.0	3.6588328615878164E-5	0.0
8	1.8294164307939082E-5	0.0	0.0	3.6588328615878164E-5	0.0
9	1.8294164307939082E-5	0.0	0.0	9.147082153969541E-5	0.0
10	3.6588328615878164E-5	0.0	0.0	9.147082153969541E-5	0.0
11	3.6588328615878164E-5	0.0	0.0	9.147082153969541E-5	0.0
12	3.6588328615878164E-5	1.8294164307939082E-5	0.0	9.147082153969541E-5	0.0
13	3.6588328615878164E-5	1.8294164307939082E-5	0.0	1.0976498584763449E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4020	0.0	11.068185	1
TAGACAG	655	0.0	9.868528	5
GTGGTAT	1820	0.0	9.716239	1
TGGTATC	1785	0.0	9.585772	2
GTACTAG	305	0.0	9.039716	1
GCGAATG	95	0.0018282703	8.998221	13
AGAGTCG	85	0.007450693	8.939574	8
TCCGCTC	130	4.3823187E-5	8.767659	8
CTATACT	695	0.0	8.753785	4
ATAGTAC	565	0.0	8.580538	3
CTTATAC	745	0.0	8.548921	3
GTACCGT	100	0.002912023	8.54831	6
TTAGACA	660	0.0	8.497846	4
TAGCACC	470	0.0	8.494781	4
TAGGACC	325	0.0	8.482334	4
TAGTACC	395	0.0	8.423096	4
GTCTTAC	475	0.0	8.406444	1
TCTACAC	645	0.0	8.400554	3
CGCCTTA	115	0.0011045664	8.265991	2
CGTCAGA	210	5.7569196E-8	8.147905	2
>>END_MODULE