FastQCFastQC Report
Wed 25 May 2016
SRR1295176_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295176_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5466224
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT201980.36950553069175357No Hit
GGTATCAACGCAGAGTACTTTTTTT165270.30234765351730924No Hit
TATCAACGCAGAGTACTTTTTTTTT117840.21557843220475414No Hit
ACGCAGAGTACTTTTTTTTTTTTTT80510.14728631684321755No Hit
GTGGTATCAACGCAGAGTACATGGG76880.14064553519943568No Hit
ATTCCATTCCATTCCATTCCATTCC63780.11668017995603547No Hit
GAATGGAATGGAATGGAATGGAATG57860.10585003468573553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCGT3950.012.7497691
GGTATCA56300.012.4726751
TGGTATC20550.011.5113872
AGCGTCA4950.011.3236633
GTGGTAT22250.010.8047331
CAGCGTC5300.010.5758732
TTATACG856.597403E-410.0590234
GTCTAGG5000.09.5021871
ACTGACG1002.7571415E-49.5001888
CCGTCCA900.00111425719.5001879
GGACGGC1106.847159E-59.4994936
CTAGGAC5750.09.0873023
ATAACGC950.00182435599.0003423
GACAACG950.00182496359.0000137
GTACTAG3600.08.9742871
AGGACGG2356.548362E-118.8937925
TACACTG12150.08.8355665
TTAGACA7250.08.7794844
TATTCCG2607.2759576E-128.7694045
TAAGACT8250.08.7516884
Produced by FastQC (version 0.11.5)