FastQCFastQC Report
Wed 25 May 2016
SRR1295175_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295175_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8771260
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCATTCCATTCCATTCCATTCC140540.16022783499748042No Hit
GAATGGAATGGAATGGAATGGAATG115760.131976477723839No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN99760.11373508481107618No Hit
GTGGTATCAACGCAGAGTACATGGG99750.1137236839405057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58500.010.4310721
AGCACCG2600.09.5044055
TCGACCG900.00111594129.498759
GTCGTAC950.00181658469.0046891
GTGGTAT30100.08.9681251
GCGCGAC850.00745237768.93943716
ACCGACC1606.766204E-78.9050268
TGGTATC27250.08.893982
CGACACG1201.7681705E-48.7071889
ACCGTTA1000.00291079688.5488258
GTATTAG13800.08.4718031
GCGTCAG2701.4551915E-118.4488441
TGGACCG2951.8189894E-128.3767645
CCGGCGT2053.8773578E-88.34050913
CGAGCCG1606.489039E-68.30964719
TATACTG18200.08.3032995
GTCTTAG9700.08.2310911
CTTAGAC5200.08.2250143
GTATCAA103600.08.1654491
CGCGACT1050.0045233698.14141317
Produced by FastQC (version 0.11.5)