FastQCFastQC Report
Wed 25 May 2016
SRR1295175_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295175_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8771260
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT247940.28267318492440086No Hit
GGTATCAACGCAGAGTACTTTTTTT178910.20397297537639975No Hit
ATTCCATTCCATTCCATTCCATTCC150020.17103586029829237No Hit
TATCAACGCAGAGTACTTTTTTTTT138360.15774244521311648No Hit
GAATGGAATGGAATGGAATGGAATG129770.14794909739307693No Hit
GTGGTATCAACGCAGAGTACATGGG112720.12851061307041406No Hit
ACGCAGAGTACTTTTTTTTTTTTTT92680.10566326844717862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA6200.011.6457563
GCGCGAC855.335376E-511.17519316
GGTATCA75750.011.0141691
TAGACCG1451.6187914E-79.8282565
GTGGTAT34800.09.529851
GTCTTAG8200.09.5025431
CGCGACT1106.852992E-59.49891417
TGGTATC32150.09.4266092
TCGACCG1151.1118576E-49.0869549
CAGCGTC7950.09.0822242
CGGCGTA1054.4964976E-49.04766812
GCAGCGT7600.08.8773771
ATCGGCG1201.765656E-48.7083310
GTCTAAG7250.08.6505921
GTACTAG7250.08.6505921
CGTGCGG1554.2134834E-68.58064310
GTATCAA138950.08.5622051
GCGTAGA1252.7381544E-48.3622381
ACTGTCC11950.08.3472788
TCGCATC1606.4607048E-68.31235514
Produced by FastQC (version 0.11.5)