FastQCFastQC Report
Wed 25 May 2016
SRR1295174_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295174_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5773028
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG75990.13162936330812877No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN65870.11409956785243378No Hit
GTATCAACGCAGAGTACTTTTTTTT65140.1128350667968352No Hit
GGTATCAACGCAGAGTACATGGGGG57830.10017273430858122No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACCG604.0762077E-412.673585
GGTATCA41250.010.5091041
CGCCTAA1054.117807E-59.95054114
GTGGTAT20000.09.6967231
GTACTAG3550.09.6404861
CTAGACT4250.09.6171784
CTAGACG1002.7421958E-49.505354
TCGACCG1106.852614E-59.4988219
TGGTATC19500.09.26170352
TAGACAG7600.09.2550495
GTTCTAG6100.09.0390541
GTCTTAG5300.08.7891121
GTCCTAG7650.08.6988411
GTATCAA76300.08.6718051
CGACCGC1107.159167E-48.63521810
GTCTAGG5300.08.4303731
CTAGACA6900.08.4032814
GTACGAC1150.001105218.2655223
TTTACGC1502.5705971E-58.237973
ATTAGAC3950.08.1818213
Produced by FastQC (version 0.11.5)