FastQCFastQC Report
Wed 25 May 2016
SRR1295174_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295174_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5773028
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT194430.33679032909592677No Hit
GGTATCAACGCAGAGTACTTTTTTT152790.26466180313000387No Hit
TATCAACGCAGAGTACTTTTTTTTT109310.18934604162668187No Hit
GTGGTATCAACGCAGAGTACATGGG86910.15054491334530162No Hit
ACGCAGAGTACTTTTTTTTTTTTTT68480.1186205921745053No Hit
GGTATCAACGCAGAGTACATGGGGG63990.11084304458596078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG853.9467413E-612.2946225
GGTATCA57100.011.981241
GTGGTAT22350.011.2661031
TGGTATC22050.010.8142222
AGCGTCA4950.09.9799483
ACCGTCC1304.24286E-69.5002268
ACCGAGT1201.7019003E-59.5002258
GCGCGAC1106.849341E-59.49923816
GTGTAGG7500.09.3750981
GTCTTAG5200.09.1363361
GGGTCGT1151.1121261E-49.086626
CGTCCTA850.00743841058.94138910
CCGCTCG1201.7649202E-48.708549
GTCCTAG6300.08.5968561
TCTGGAC9150.08.513793
GGACTGT8750.08.4682586
TGTACCG3150.08.4447915
GTATCAA101950.08.3041631
GTTCTAA5750.08.2624241
CTAGTAC4100.08.1098793
Produced by FastQC (version 0.11.5)