FastQCFastQC Report
Wed 25 May 2016
SRR1295173_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295173_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5173812
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG73630.14231286332012064No Hit
GTATCAACGCAGAGTACTTTTTTTT59300.114615683755034No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN57440.11102065556305486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC907.481478E-611.6094878
GTGGTAT18300.011.375871
CGTACGA600.005884065311.08199710
TGGTATC18700.010.7753412
GCGGTAT803.752413E-410.6940971
CCTACGA909.4766976E-510.5607382
GGTATCA40700.010.5101691
CCGTCCC1701.5661499E-910.0574169
TATGCCG800.0044976669.505315
CCGTCCA800.0045236639.4986719
GTGTTAT5900.09.0225141
TCGGTCG950.00182711328.9988296
TGTACCG1701.6732156E-78.9461745
CCCCGCG1606.7657675E-78.90483112
AGCCTCG5550.08.7340162
GTAAGAC5600.08.6562853
GTACCGT1356.814623E-58.4433456
TAGGACT6200.08.432134
ACACCGT2159.757059E-98.3942566
CTCGGTC2502.3283064E-108.3646735
Produced by FastQC (version 0.11.5)