##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295173_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5173812
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.920057976594432	31.0	30.0	33.0	25.0	34.0
2	29.89759349585953	31.0	30.0	33.0	25.0	34.0
3	29.990608278770083	31.0	30.0	34.0	25.0	34.0
4	33.427107131067	35.0	33.0	37.0	27.0	37.0
5	33.534262164918246	35.0	33.0	37.0	28.0	37.0
6	33.86756959858611	36.0	35.0	37.0	28.0	37.0
7	33.66079691337838	35.0	35.0	37.0	28.0	37.0
8	33.76268909655009	36.0	35.0	37.0	28.0	37.0
9	34.90175271154035	39.0	35.0	39.0	27.0	39.0
10	34.885946764204036	38.0	35.0	39.0	27.0	39.0
11	35.338802414931195	39.0	35.0	39.0	27.0	39.0
12	35.35072824447429	39.0	35.0	39.0	27.0	39.0
13	35.27572861170835	39.0	35.0	39.0	27.0	39.0
14	35.89556230493106	39.0	35.0	41.0	27.0	41.0
15	36.270709681758824	39.0	36.0	41.0	27.0	41.0
16	36.22824949959527	39.0	36.0	41.0	27.0	41.0
17	35.869736666117745	39.0	35.0	41.0	27.0	41.0
18	35.76957782772161	39.0	35.0	41.0	26.0	41.0
19	36.1993462460561	39.0	36.0	41.0	27.0	41.0
20	36.26641265666398	39.0	36.0	41.0	27.0	41.0
21	36.2960905807942	39.0	36.0	41.0	27.0	41.0
22	36.190772490380404	39.0	36.0	41.0	27.0	41.0
23	35.88420878068241	39.0	35.0	41.0	26.0	41.0
24	36.20082233370675	39.0	36.0	41.0	27.0	41.0
25	34.85847301757389	38.0	33.0	41.0	23.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.15050860504504726
1101	2	-0.011235645986825915
1101	3	-0.18528917487476093
1101	4	-0.016072824586167656
1101	5	-0.27815206991128605
1101	6	-0.29410299954096075
1101	7	-0.29520775924924436
1101	8	-0.24171473687265177
1101	9	-0.08255328980850152
1101	10	0.05401954189228064
1101	11	-0.017551891668993846
1101	12	-0.005873209363102205
1101	13	-0.015273340083062692
1101	14	0.37161349225155504
1101	15	0.013749624785482695
1101	16	-0.005499241956066214
1101	17	0.039313546134600585
1101	18	0.32010923925746937
1101	19	0.09662320823742476
1101	20	0.12197678857251049
1101	21	0.145190170702719
1101	22	0.011630863530221802
1101	23	0.0651349575045046
1101	24	-0.12036118575732502
1101	25	-0.1843797707832593
1102	1	-0.29677504785151854
1102	2	-0.13461900926324688
1102	3	-0.2842450662993379
1102	4	-0.18230386822393285
1102	5	-0.6288148888574625
1102	6	-0.3875314901561566
1102	7	-0.5090490573032724
1102	8	-0.3783860049090535
1102	9	-0.335177040687185
1102	10	0.07726558371835068
1102	11	-0.09248520649831704
1102	12	-0.17275772906200615
1102	13	-0.22188867563932746
1102	14	-0.006161891949162168
1102	15	-0.0976451465812076
1102	16	-0.25235990941199304
1102	17	-0.3805787224155921
1102	18	-0.014604412733334016
1102	19	-0.2519222591458288
1102	20	-0.12953809152537588
1102	21	-0.09846663865511829
1102	22	-0.23921487446317968
1102	23	-0.25263705877367926
1102	24	-0.21972289966896597
1102	25	-0.3164058908935914
1103	1	-0.4944410743547927
1103	2	-0.3851320824236666
1103	3	-0.62356525910063
1103	4	-0.4367828484055849
1103	5	-0.6577375476699814
1103	6	-0.6453244929016861
1103	7	-0.7530245910017257
1103	8	-0.5921313158879471
1103	9	-0.08221635151434015
1103	10	-0.007652326956602451
1103	11	-0.2503385426200566
1103	12	-0.30069430042843237
1103	13	-0.24270024553187142
1103	14	-0.3865640810351749
1103	15	-0.3244921854523213
1103	16	-0.37630976431584884
1103	17	-0.27731955779619
1103	18	0.001548362959063354
1103	19	-0.28156679271704377
1103	20	-0.1514511474388911
1103	21	-0.27034481004688615
1103	22	-0.2258108075141365
1103	23	-2.5444804971633204
1103	24	-1.2152888642396462
1103	25	-0.6848812733104879
1104	1	-0.28501965764300863
1104	2	-0.14884941239604288
1104	3	-0.3227145597010974
1104	4	-0.6224553728347217
1104	5	-0.4800798131061583
1104	6	-0.5133247557775746
1104	7	-0.4793657182266031
1104	8	-0.5471204438711723
1104	9	-2.7400260964679575
1104	10	-0.9475441555938673
1104	11	-0.6459404294771076
1104	12	-0.4146766309711154
1104	13	-0.3079927319437843
1104	14	-0.06830275728648871
1104	15	-0.25133140054758485
1104	16	-0.3009084269863891
1104	17	0.03092246143469879
1104	18	0.10963645872433858
1104	19	-0.1507579108840602
1104	20	-0.2755831882073636
1104	21	-0.30486757074604753
1104	22	-0.2583545153613045
1104	23	-0.20664919913965463
1104	24	-0.23058985798963505
1104	25	-0.30197501867080945
1105	1	-0.2813926601738217
1105	2	-0.3162035843349358
1105	3	-0.31471537021639406
1105	4	-0.16838321328952333
1105	5	-0.3319269882834561
1105	6	-0.3341226308941856
1105	7	-0.4199118774674204
1105	8	-0.48170283916901013
1105	9	-2.1812983423277714
1105	10	-0.7184280017637974
1105	11	-0.4940852427128348
1105	12	-0.3787907592439339
1105	13	-0.23262665139488092
1105	14	-0.2596258869573873
1105	15	-0.26433280272150483
1105	16	-0.2653151390003927
1105	17	-1.654222243469171
1105	18	-0.39252159672900433
1105	19	-0.30164293540053677
1105	20	-0.2826645246745727
1105	21	-0.2687214247799048
1105	22	-0.1750235138676146
1105	23	-0.1471574495391792
1105	24	-0.22449154909237024
1105	25	-0.7692377883436734
1106	1	-0.40894196174821573
1106	2	-0.6729983070958632
1106	3	-0.5794909108019191
1106	4	-0.47987617245981795
1106	5	-0.4615790518651224
1106	6	-0.4619193880650272
1106	7	-0.6288001071891927
1106	8	-0.5120674292608811
1106	9	-3.1929124170159575
1106	10	-0.9329776921660695
1106	11	-0.838317818513211
1106	12	-0.5766675008833033
1106	13	-0.6409460068386679
1106	14	-0.9189871596850949
1106	15	-0.8019561547146097
1106	16	-0.7027889031657395
1106	17	-0.5646577391961216
1106	18	-0.1698085302960095
1106	19	-0.4771689756233144
1106	20	-0.46004291896768024
1106	21	-0.38562382913663384
1106	22	-0.43131017901895063
1106	23	-0.20618048606465322
1106	24	-0.5194168390342355
1106	25	-0.7449322047181326
1107	1	-0.3885203705687914
1107	2	-0.13279983371273474
1107	3	-0.2290534700964848
1107	4	-1.5025809424799483
1107	5	-0.4995572129348105
1107	6	-0.3992034589111455
1107	7	-0.295039242706423
1107	8	-0.17489426730193713
1107	9	0.07193490545985526
1107	10	0.30785989593638874
1107	11	0.05415928837731343
1107	12	-0.5638536699473917
1107	13	-0.15971855476370678
1107	14	-0.05820740633946997
1107	15	-0.6794584349172936
1107	16	-0.9777614471643901
1107	17	-0.18242374482498747
1107	18	-0.2542360500406673
1107	19	-0.09529190582687619
1107	20	-0.4733550610456376
1107	21	-0.2748820810817705
1107	22	-0.24077510078971898
1107	23	0.019866965378966484
1107	24	-0.24088776489881525
1107	25	-0.3501378653282714
1108	1	0.27270646787792074
1108	2	0.2969749286494796
1108	3	0.3748221843378161
1108	4	0.7411586127465242
1108	5	0.5875078270726632
1108	6	0.549132993844772
1108	7	0.501216650639428
1108	8	0.5291369687547061
1108	9	0.8229821572430893
1108	10	0.8933457278123171
1108	11	0.6204310141403013
1108	12	0.5512354204091494
1108	13	0.48819365260406045
1108	14	0.5551151256877773
1108	15	0.5701761820459765
1108	16	0.5826893545232323
1108	17	0.7484135546606865
1108	18	0.8491363953749058
1108	19	0.5995268875573245
1108	20	0.5546500608436844
1108	21	0.5090460116340338
1108	22	0.5535261747491802
1108	23	0.6499041154544756
1108	24	0.5120033997102809
1108	25	0.5817822251626623
1109	1	0.3975358099027986
1109	2	0.2958027759895572
1109	3	0.45215359215385575
1109	4	0.7115721507222972
1109	5	0.6065537344514951
1109	6	0.5110690212269375
1109	7	0.5158267528666016
1109	8	0.41481580115212324
1109	9	0.7937291301738654
1109	10	0.582805759692782
1109	11	0.4730706880307807
1109	12	0.42611464210560257
1109	13	0.41091254901299124
1109	14	0.8893083116358653
1109	15	0.48270778076976484
1109	16	0.5602059632301533
1109	17	0.7452486554276092
1109	18	0.6036413171046036
1109	19	0.44388524446692657
1109	20	0.454176868621623
1109	21	0.4854969271188381
1109	22	0.4534454125646761
1109	23	0.81243255678541
1109	24	0.5599091192321595
1109	25	0.6139456334926692
1110	1	0.2957140492738368
1110	2	0.27406004292453545
1110	3	0.29081196877350735
1110	4	0.5144267404159848
1110	5	0.5401218203601417
1110	6	0.41337887232165826
1110	7	0.4284044546771
1110	8	0.482113253298337
1110	9	0.9008707779387493
1110	10	0.8539203041820898
1110	11	0.5139947205841651
1110	12	0.3942884776027995
1110	13	0.4336880039643276
1110	14	0.6670923221759679
1110	15	0.5101128070141314
1110	16	0.4757336348264758
1110	17	0.8838568986231792
1110	18	0.9266050381697255
1110	19	0.4906274133969575
1110	20	0.6657715358759759
1110	21	0.5190723156427381
1110	22	0.6088886316316362
1110	23	0.6682272833702569
1110	24	0.48383536322368315
1110	25	0.42160096129217806
1111	1	0.05576662246308217
1111	2	0.0947065292119369
1111	3	0.2285674237191131
1111	4	0.516046493202964
1111	5	0.49519262910243356
1111	6	0.28473400747136424
1111	7	0.3126143072655623
1111	8	0.1908996682604709
1111	9	0.6277041022890515
1111	10	0.5158888456073356
1111	11	0.27916425283773805
1111	12	0.1620249109801506
1111	13	0.189187303287909
1111	14	0.09147821971551195
1111	15	0.24558556148094368
1111	16	0.12410635875098563
1111	17	0.44061786465833563
1111	18	0.5631768664860388
1111	19	0.3097237653880427
1111	20	-0.2684131642255281
1111	21	-0.3531794382940703
1111	22	-0.19357507692290454
1111	23	0.16775398992157875
1111	24	0.273472426311514
1111	25	0.15515640006833564
1112	1	0.22227611199888742
1112	2	0.2527070326071801
1112	3	0.34592224238972236
1112	4	0.6259636471990717
1112	5	0.5493705418329782
1112	6	0.4688529586955781
1112	7	0.4317374355992172
1112	8	0.45260441088626635
1112	9	-1.250599295093295
1112	10	0.1172790733133553
1112	11	0.25122447818743865
1112	12	0.17928467363464762
1112	13	0.1357628768371626
1112	14	0.5401664466932132
1112	15	0.37981456347310427
1112	16	0.30486166582208085
1112	17	0.35828676982724517
1112	18	0.5103366998300842
1112	19	0.3070530929805315
1112	20	0.3023883052601519
1112	21	0.263642798922902
1112	22	0.3385305611037879
1112	23	0.1267275456056609
1112	24	0.1879645861984116
1112	25	0.2729771270995869
1113	1	0.2958197653246373
1113	2	0.29913584212872024
1113	3	0.33282650921227486
1113	4	0.5734111318261981
1113	5	0.6457706819374636
1113	6	0.3950911366237406
1113	7	0.4392978473443492
1113	8	0.3475612540788404
1113	9	0.21190112251422022
1113	10	0.5814767385510109
1113	11	0.3755263177919872
1113	12	0.18865626994446671
1113	13	0.18665244508466117
1113	14	-0.03973034340538106
1113	15	0.04637073988442353
1113	16	0.2089739622582556
1113	17	0.6126550048689978
1113	18	0.41700951609804804
1113	19	0.2535674799183454
1113	20	0.33391572986668905
1113	21	0.23912938159036656
1113	22	0.28786617589687324
1113	23	0.01673627265784461
1113	24	0.09682882528426262
1113	25	0.13864641853339066
1114	1	0.474335577356225
1114	2	0.5176318241277151
1114	3	0.5984623146106465
1114	4	0.8252544273409157
1114	5	0.7544922522738347
1114	6	0.6025987046753656
1114	7	0.4974402906637678
1114	8	0.5000357620410227
1114	9	0.12201130943220306
1114	10	0.4915756986738131
1114	11	0.5297556396973206
1114	12	0.48917738139969913
1114	13	0.46217806920980564
1114	14	0.8916024220653611
1114	15	0.5414082298540066
1114	16	0.4342241473122215
1114	17	0.8157374751502928
1114	18	0.706685386604299
1114	19	0.6269989272880707
1114	20	0.5760198746364011
1114	21	0.5882784469628035
1114	22	0.34543831056472385
1114	23	0.7796105373184403
1114	24	0.7192222174560428
1114	25	0.583875212465756
1115	1	0.4950582967016004
1115	2	0.5012879219165463
1115	3	0.6125205815046186
1115	4	0.8466123142053519
1115	5	0.8991894131750229
1115	6	0.6016000497208154
1115	7	0.7231406975322798
1115	8	0.6132606788273023
1115	9	0.8510547168256224
1115	10	0.5840342827837972
1115	11	0.5565435152945568
1115	12	0.6189874285691914
1115	13	0.5497578957990754
1115	14	0.6065298845798281
1115	15	0.5627832136602962
1115	16	0.6513987931539091
1115	17	0.8824031611621805
1115	18	0.9249183654051265
1115	19	0.598123611865617
1115	20	0.7094664735965921
1115	21	0.656595762074538
1115	22	0.06329141467258381
1115	23	0.6077244001124527
1115	24	0.6497949559757998
1115	25	0.6588555606601361
1116	1	0.8134764695845078
1116	2	0.7593934901762154
1116	3	0.969116486535615
1116	4	1.1272171647227296
1116	5	1.152974322719949
1116	6	0.9619627822735524
1116	7	1.0391086096626339
1116	8	0.8091106231868039
1116	9	1.1123628557429939
1116	10	0.9373558755083451
1116	11	0.6893825936287499
1116	12	0.6658295775241427
1116	13	0.6274283955940092
1116	14	1.147467715391258
1116	15	0.8346453896776822
1116	16	0.9449508935552089
1116	17	1.3308302662387348
1116	18	1.3742767761262584
1116	19	0.9081831984851547
1116	20	0.9526221508892334
1116	21	0.9356974263113713
1116	22	1.0450808625121084
1116	23	1.256974266566118
1116	24	0.9725387397671881
1116	25	0.9240173208105418
1201	1	-0.24330969792189805
1201	2	-0.03787922842243319
1201	3	-0.3257451159771456
1201	4	-0.08449846868176536
1201	5	-0.5265841548113741
1201	6	-0.3692464897486971
1201	7	-0.47653168991129036
1201	8	-0.44817209777201583
1201	9	0.061168630467733465
1201	10	0.1982152143480178
1201	11	0.0383251716920725
1201	12	0.04235389778761345
1201	13	0.020016171435287333
1201	14	0.4200379708997133
1201	15	0.10437894271728965
1201	16	0.06348957321846171
1201	17	0.14764962372211698
1201	18	0.3323273528552164
1201	19	0.0895446572454972
1201	20	0.0012797805063016199
1201	21	-0.019417162462183057
1201	22	-0.060925702391891434
1201	23	-0.12517944176930484
1201	24	-0.18565697560049443
1201	25	-0.1933244421576461
1202	1	-0.5103931762206528
1202	2	-0.5116706284045236
1202	3	-0.6594800686225213
1202	4	-0.42912507723794846
1202	5	-0.9139471012411704
1202	6	-0.6783493045725706
1202	7	-0.7274186598901338
1202	8	-0.6425895837347966
1202	9	-0.057491116833524814
1202	10	0.09015999202860314
1202	11	-0.2501416790631765
1202	12	-0.3478075042333728
1202	13	-0.2450264513381768
1202	14	-0.34868661955302116
1202	15	-0.3681848920460453
1202	16	-0.3111182641707444
1202	17	0.027164530087198102
1202	18	0.09708421665637701
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2211	10	-0.0395227395095219
2211	11	-0.09103295049565219
2211	12	-0.18965085709164242
2211	13	-0.08428299913401816
2211	14	0.2024399644132231
2211	15	-0.045618830574127855
2211	16	0.06356083912503863
2211	17	0.26641377967798263
2211	18	0.35350419389534693
2211	19	0.0820446085274682
2211	20	0.14361733930699216
2211	21	-0.06422286346514738
2211	22	-0.006904656991238767
2211	23	-2.106492807844525
2211	24	-0.7301775568180418
2211	25	-0.46880920616388266
2212	1	0.05159966971587693
2212	2	-1.3409213947938632
2212	3	-0.2884972510330073
2212	4	0.25248016011788366
2212	5	0.3553175067592136
2212	6	0.32766456626721663
2212	7	0.30997223675242935
2212	8	0.29272403594659124
2212	9	0.676360110529103
2212	10	0.5469136374633123
2212	11	0.27385907905374296
2212	12	0.18811088728894276
2212	13	0.08779958791305376
2212	14	0.4646517852434897
2212	15	0.22892196872044224
2212	16	0.2544149917741123
2212	17	0.6344502573121673
2212	18	0.7310001304851284
2212	19	0.49080892023701495
2212	20	0.33156885940775993
2212	21	0.29429468367805356
2212	22	0.3348091769942343
2212	23	0.5143849449798452
2212	24	0.3704998364466121
2212	25	0.25403894030468166
2213	1	0.3054724159375475
2213	2	0.15801945429514674
2213	3	0.3066518572678092
2213	4	0.5311553017357014
2213	5	0.5445091974519798
2213	6	0.4210915497987955
2213	7	0.3497034821678824
2213	8	0.31866244310150904
2213	9	0.5771958113044349
2213	10	0.6383085532947987
2213	11	0.3195152364345262
2213	12	0.38561601817559676
2213	13	0.16129939757018974
2213	14	-1.2542899944881185
2213	15	-0.20063848663060213
2213	16	0.12073761423077656
2213	17	0.6302810540486021
2213	18	0.317606988978099
2213	19	0.18766476660530884
2213	20	0.3202903692320831
2213	21	0.35721014860378375
2213	22	0.411605618021639
2213	23	0.6073480168374843
2213	24	0.35202876074293243
2213	25	0.22637140385630516
2214	1	0.4985186995493258
2214	2	0.4982998205738838
2214	3	0.5032487939402337
2214	4	0.7943684178263339
2214	5	0.6590413217931683
2214	6	0.45827134075042153
2214	7	0.4148975191622739
2214	8	0.3938947090333258
2214	9	0.5344367676079287
2214	10	0.41169984771947554
2214	11	0.3467908228073924
2214	12	0.30634989752488195
2214	13	0.15584057122185868
2214	14	0.3985661493229813
2214	15	0.19086021854123203
2214	16	0.34266060722341507
2214	17	0.31081269466969275
2214	18	0.5166004405668971
2214	19	0.2403177645648995
2214	20	0.4382490022760521
2214	21	0.46654287438372677
2214	22	0.45839001400756274
2214	23	0.5817298857710256
2214	24	0.5508168653286205
2214	25	0.5291885253674025
2215	1	0.5002449964345708
2215	2	0.41261810765700346
2215	3	0.47748336929277
2215	4	0.685659715913971
2215	5	0.6899917035145933
2215	6	0.5321534314856464
2215	7	0.6528171019960283
2215	8	0.490971318727901
2215	9	0.7331615399311673
2215	10	0.7277081057939228
2215	11	0.45736297409303006
2215	12	0.38522867457369614
2215	13	0.21630254969596763
2215	14	0.47913441628556797
2215	15	0.36904134539067
2215	16	0.4053562983781589
2215	17	0.6237360050980882
2215	18	0.7991951241939574
2215	19	0.38182002575043583
2215	20	-0.461057774043212
2215	21	0.14307153008601858
2215	22	-0.6947413591354277
2215	23	0.28253419903371935
2215	24	0.31195901485091326
2215	25	0.38950172682537243
2216	1	0.393999221753095
2216	2	0.05014364766348223
2216	3	0.46542561019724005
2216	4	0.6723127758060627
2216	5	0.7228935482199432
2216	6	0.5473375925738679
2216	7	0.6709014197858494
2216	8	0.48801567390864875
2216	9	0.8465217164883541
2216	10	0.6445558698199818
2216	11	0.47488485655578927
2216	12	-0.009358191058176146
2216	13	0.20489150476339546
2216	14	0.2802189495962111
2216	15	0.3172476472389931
2216	16	0.4004068128916529
2216	17	0.4504136666437475
2216	18	0.8526602919149084
2216	19	0.6008698064495022
2216	20	-0.6259875119487788
2216	21	-0.8432972631781723
2216	22	-0.8350743885633634
2216	23	-1.3522334237679416
2216	24	-1.0627837648070724
2216	25	-0.8398368523851403
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	10274.0
3	1330.0
4	1248.0
5	955.0
6	1116.0
7	1374.0
8	702.0
9	1491.0
10	3342.0
11	6533.0
12	11584.0
13	17384.0
14	23528.0
15	29061.0
16	32497.0
17	34769.0
18	36075.0
19	36222.0
20	36266.0
21	36990.0
22	37760.0
23	39502.0
24	41723.0
25	45187.0
26	48923.0
27	55257.0
28	64471.0
29	77508.0
30	95736.0
31	121158.0
32	157602.0
33	210397.0
34	286077.0
35	403860.0
36	595216.0
37	964708.0
38	1605986.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.11698441885222	20.684293041711022	13.579657408303495	23.61906513113326
2	15.723565694278127	21.541324542282446	39.861947214153545	22.873162549285876
3	18.59150253021872	26.193136020493103	29.656816540330283	25.5585449089579
4	13.184319499937432	17.46814225476302	37.07637225715896	32.27116598814059
5	13.767258183649716	37.34663084051314	34.41235400839208	14.473756967445063
6	31.145832767690152	37.75191161537608	17.719105603707412	13.383150013226352
7	26.10893155428745	32.31664425695666	23.251000194521325	18.323423994234567
8	24.247663879525355	36.59382830469831	20.884628022738355	18.273879793037977
9	24.973211014583487	17.135020559394746	21.576532519064546	36.31523590695722
10	15.559780652783989	29.402848254447427	33.22200417702597	21.815366915742615
11	32.45964400463288	25.39480787590077	23.451885379765542	18.6936627397008
12	21.39022281041692	26.971275925005433	31.220280097985615	20.418221166592033
13	26.442036910762766	24.09417132256813	26.695110367985052	22.76868139868405
14	21.32115938690498	23.038685592282544	27.558542422763484	28.081612598048995
15	22.913907695853062	30.453354148751743	24.056104639377338	22.576633516017854
16	22.73205094500139	30.666770585411513	25.11979737395781	21.48138109562929
17	21.17138655593405	29.71298529011544	27.78993261420756	21.325695539742952
18	21.180477989997147	29.69847552651501	28.40223869714683	20.718807786341014
19	21.741378491101397	28.317376644760767	28.61195718894639	21.32928767519145
20	22.8387748664892	28.048889951275612	28.045671772520514	21.06666340971467
21	23.28151483055984	28.44744840437633	27.02086051440174	21.250176250662093
22	22.38115703587994	27.97678554016286	28.473498834643884	21.168558589313307
23	22.641076442338072	28.391124025703935	27.734454194565767	21.23334533739223
24	22.316501011459458	27.501789177063863	28.04997247643628	22.131737335040395
25	22.846864788977616	27.872667841991515	27.42303144236323	21.85743592666764
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13836.0
1	13836.0
2	11631.5
3	9427.0
4	9427.0
5	9427.0
6	9769.0
7	10111.0
8	10111.0
9	10111.0
10	13868.0
11	17625.0
12	17625.0
13	17625.0
14	28220.0
15	38815.0
16	38815.0
17	38815.0
18	62166.5
19	85518.0
20	85518.0
21	85518.0
22	125356.0
23	165194.0
24	165194.0
25	165194.0
26	218674.5
27	272155.0
28	272155.0
29	272155.0
30	333657.0
31	395159.0
32	395159.0
33	395159.0
34	454833.5
35	514508.0
36	514508.0
37	514508.0
38	563342.0
39	612176.0
40	612176.0
41	612176.0
42	621443.0
43	630710.0
44	630710.0
45	630710.0
46	612928.0
47	595146.0
48	595146.0
49	595146.0
50	572315.5
51	549485.0
52	549485.0
53	549485.0
54	504862.0
55	460239.0
56	460239.0
57	460239.0
58	400567.0
59	340895.0
60	340895.0
61	340895.0
62	284077.0
63	227259.0
64	227259.0
65	227259.0
66	177560.5
67	127862.0
68	127862.0
69	127862.0
70	94921.0
71	61980.0
72	61980.0
73	61980.0
74	44880.5
75	27781.0
76	27781.0
77	27781.0
78	19637.0
79	11493.0
80	11493.0
81	11493.0
82	7947.0
83	4401.0
84	4401.0
85	4401.0
86	2940.0
87	1479.0
88	1479.0
89	1479.0
90	968.0
91	457.0
92	457.0
93	457.0
94	268.5
95	80.0
96	80.0
97	80.0
98	50.5
99	21.0
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14132712978361023
2	0.2826929157843385
3	0.3428033334029145
4	0.3722013865211956
5	0.3800485985961608
6	0.3370822132694423
7	0.3394982268393208
8	0.35699016508524084
9	0.34355712963671664
10	0.3754871649762303
11	0.29063676840209884
12	0.33911166466814024
13	0.3096556272241821
14	0.32024743071452927
15	0.36064317760289705
16	0.30801273799666473
17	0.3589423040497026
18	0.32633578491062293
19	0.3414696939123416
20	0.30180841514921686
21	0.34409831667636936
22	0.29577804527880025
23	0.27921385624371353
24	0.3172322457793209
25	0.310969938606196
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5173812.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.84804127816814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.76427685847254	56.293103082316854
2	11.786835263706596	16.230010415492792
3	3.174319609826426	6.556370625822851
4	1.2157313689353992	3.348028938865129
5	0.6080894805120904	2.093288482755811
6	0.34754743539108507	1.435677610675614
7	0.23289908387516384	1.1224252018199377
8	0.16527442386899482	0.910305628540641
9	0.11411291683568003	0.7070805727807561
>10	0.5443518251194976	6.553535674577642
>50	0.03090101389373191	1.4582480533163225
>100	0.014420107090782919	1.7421335161659743
>500	6.766983809566175E-4	0.3461393366837725
>1k	4.5113127287642573E-4	0.7351008226708161
>5k	1.1278281821658211E-4	0.4685520375150876
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	7363	0.14231286332012064	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5930	0.114615683755034	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	5744	0.11102065556305486	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.9328108559027658E-5	0.0
3	0.0	0.0	0.0	1.9328108559027658E-5	0.0
4	0.0	0.0	0.0	1.9328108559027658E-5	0.0
5	0.0	0.0	0.0	1.9328108559027658E-5	0.0
6	0.0	0.0	0.0	3.8656217118055316E-5	0.0
7	0.0	0.0	0.0	3.8656217118055316E-5	0.0
8	0.0	0.0	0.0	3.8656217118055316E-5	0.0
9	0.0	0.0	0.0	7.731243423611063E-5	0.0
10	0.0	0.0	0.0	7.731243423611063E-5	0.0
11	0.0	0.0	0.0	7.731243423611063E-5	0.0
12	0.0	0.0	0.0	7.731243423611063E-5	0.0
13	0.0	0.0	0.0	7.731243423611063E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTCC	90	7.481478E-6	11.609487	8
GTGGTAT	1830	0.0	11.37587	1
CGTACGA	60	0.0058840653	11.081997	10
TGGTATC	1870	0.0	10.775341	2
GCGGTAT	80	3.752413E-4	10.694097	1
CCTACGA	90	9.4766976E-5	10.560738	2
GGTATCA	4070	0.0	10.510169	1
CCGTCCC	170	1.5661499E-9	10.057416	9
TATGCCG	80	0.004497666	9.50531	5
CCGTCCA	80	0.004523663	9.498671	9
GTGTTAT	590	0.0	9.022514	1
TCGGTCG	95	0.0018271132	8.998829	6
TGTACCG	170	1.6732156E-7	8.946174	5
CCCCGCG	160	6.7657675E-7	8.904831	12
AGCCTCG	555	0.0	8.734016	2
GTAAGAC	560	0.0	8.656285	3
GTACCGT	135	6.814623E-5	8.443345	6
TAGGACT	620	0.0	8.43213	4
ACACCGT	215	9.757059E-9	8.394256	6
CTCGGTC	250	2.3283064E-10	8.364673	5
>>END_MODULE