FastQCFastQC Report
Wed 25 May 2016
SRR1295173_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295173_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5173812
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT172830.334047700225675No Hit
GGTATCAACGCAGAGTACTTTTTTT141310.2731255020476198No Hit
TATCAACGCAGAGTACTTTTTTTTT99400.19212139907673492No Hit
GTGGTATCAACGCAGAGTACATGGG83180.16077120699399206No Hit
ACGCAGAGTACTTTTTTTTTTTTTT62860.12149649040204785No Hit
ATTCCATTCCATTCCATTCCATTCC56130.10848867334182224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT22950.011.6353541
GGTATCA53150.011.4070511
TAGACCG600.005873920411.0845685
TAAGCCG600.005874765611.0843534
TGGTATC23000.010.6988112
CCGCGTC1002.4012026E-510.4500539
TATTCCG2200.010.3647915
AGCGTCA4550.010.0229983
ACCGACC1151.0275586E-59.9130948
GTCCTAG4750.09.4028651
TACCGTC1054.4961536E-49.0475787
TACCGTG1804.226422E-88.9721817
TCGTATA850.0074340248.9419992
TGTCCGT1304.3727097E-58.76927610
TACACCG2106.379196E-98.5961965
GTATACG1554.194806E-68.5832611
CGTATAT1000.00289875978.5526071
GGTCGAA1000.00290663358.55004311
CCATACG1000.00290663358.5500439
GCAGCGT4900.08.5332131
Produced by FastQC (version 0.11.5)